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A new method for generation and screening of Chinese cabbage mutants using isolated microspore culturing and EMS mutagenesis

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Abstract

A new method for generation and screening Chinese cabbage mutants was employed in this study. In order to quickly obtain homozygous mutants in Chinese cabbage, isolated microspore culturing and ethyl methanesulfonate mutagenesis were performed on a doubled haploid line. Microspores were treated with different concentrations (0.04, 0.08, 0.12 %, v/v) of ethyl methanesulfonate solution for 10 min, and mutagenic microspores were cultured in NLN-13 medium. A total of six stable inheriting mutants were selected, which accounted for 0.46 % of 1304 regenerated plants. The mutants exhibited variation in traits including overall plant shape, floral organs and leaves. These results could be applied in future functional genomics studies on Chinese cabbage.

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Acknowledgments

This work was supported by a Grant from the National Natural Science Foundation of China (31272157) and the Postgraduate Innovation Cultivation Project of Shenyang Agricultural University.

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    Authors and Affiliations

    1. Department of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenhe District, Shenyang, 110866, People’s Republic of China

      Shengnan Huang, Zhiyong Liu, Danyang Li, Runpeng Yao & Hui Feng

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    1. Shengnan Huang
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    2. Zhiyong Liu
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    3. Danyang Li
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    5. Hui Feng
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    Correspondence to Hui Feng.

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    Shengnan Huang and Zhiyong Liu have contributed equally to this work and share the first authorship.

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    Huang, S., Liu, Z., Li, D. et al. A new method for generation and screening of Chinese cabbage mutants using isolated microspore culturing and EMS mutagenesis. Euphytica 207, 23–33 (2016). https://doi.org/10.1007/s10681-015-1473-5

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