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A comparison of aggressiveness and deoxynivalenol production between Canadian Fusarium graminearum isolates with 3-acetyl and 15-acetyldeoxynivalenol chemotypes in field-grown spring wheat

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Abstract

Twenty four isolates of Fusarium graminearum, half of which were 3-acetyldeoxynivalenol (3-ADON) and half 15-acetyldeoxynivalenol (15-ADON) chemotypes, were tested for their ability to produce deoxynivalenol and to cause Fusarium head blight (FHB) in spring wheat cultivars. The objectives of this study were to determine (1) whether 3-ADON isolates differ in aggressiveness, as measured by the FHB index, and DON production from 15-ADON isolates under field conditions, and (2) whether the performance of resistant host cultivars was stable across isolates. Field tests of all isolates were conducted with three replicates at each of two locations in Canada and Germany in 2008 with three host genotypes differing in FHB resistance level. The resistant host genotype showed resistance regardless of the chemotype or location. The differences between mean FHB indices of 3-ADON and 15-ADON isolates were not significant for any wheat genotype. In contrast, average DON production by the 3-ADON isolates (10.44 mg kg−1) was significantly (P < 0.05) higher than for the 15-ADON isolates (6.95 mg kg−1) at three of the four locations where moderately resistant lines were tested, and at both locations where susceptible lines were evaluated. These results indicate that 3-ADON isolates could pose a greater risk to food safety. However, as the mean aggressiveness and DON production of 3-ADON and 15-ADON chemotypes was similar on highly resistant lines, breeding and use of highly resistant lines is still the most effective measure of reducing the risks associated with DON in wheat.

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Acknowledgements

This research was funded by the Federal Ministry of Education and Research (BMBF) in Germany within the framework of GABI CANADA (grant no. 0313711A), the National Science and Engineering Research Council, Husky Energy Inc., Western Grains Research Foundation, Agri Food Research & Development Initiative, Ontario Wheat Producers Marketing Board (OWMPB), and the Canadian Agricultural Adaptation Council. The authors wish to thank all technicians and students who helped in conducting the field trials and disease evaluations at each location.

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Authors and Affiliations

  1. State Plant Breeding Institute, Universitaet Hohenheim, 70599, Stuttgart, Germany

    Christiane von der Ohe & Thomas Miedaner

  2. Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada

    Victoria Gauthier, Anita Brule-Babel & W. G. Dilantha Fernando

  3. Department of Plant Agriculture, University of Guelph, Ridgetown, Ontario N0P 2C0, Canada

    Lily Tamburic-Ilincic

  4. Grain Research Laboratory, Canadian Grain Commission, Winnipeg, MB R3C 3G8, Canada

    Randy Clear

  5. Bacterial Foodborne Pathogens and Mycology Research Unit, United States Department of Agriculture - Agriculture Research Service, Peoria, IL 61604, USA

    Todd J. Ward

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  1. Christiane von der Ohe
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  2. Victoria Gauthier
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  3. Lily Tamburic-Ilincic
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  4. Anita Brule-Babel
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  5. W. G. Dilantha Fernando
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  6. Randy Clear
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  7. Todd J. Ward
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  8. Thomas Miedaner
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Correspondence to Thomas Miedaner.

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von der Ohe, C., Gauthier, V., Tamburic-Ilincic, L. et al. A comparison of aggressiveness and deoxynivalenol production between Canadian Fusarium graminearum isolates with 3-acetyl and 15-acetyldeoxynivalenol chemotypes in field-grown spring wheat. Eur J Plant Pathol 127, 407–417 (2010). https://doi.org/10.1007/s10658-010-9607-z

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