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Feeding strategies for the enhanced production of α-arbutin in the fed-batch fermentation of Xanthomonas maltophilia BT-112

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Abstract

To develop a cost-effective method for the enhanced production of α-arbutin using Xanthomonas maltophilia BT-112 as a biocatalyst, different fed-batch strategies such as constant feed rate fed-batch, constant hydroquinone (HQ) concentration fed-batch, exponential fed-batch and DO-control pulse fed-batch (DPFB) on α-arbutin production were investigated. The research results indicated that DPFB was an effective method for α-arbutin production. When fermentation with DO-control pulse feeding strategy to feed HQ and yeast extract was applied, the maximum concentrations of α-arbutin and cell dry weight were 61.7 and 4.21 g/L, respectively. The α-arbutin production was 394 % higher than that of the control (batch culture) and the molar conversion yield of α-arbutin reached 94.5 % based on the amount of HQ supplied (240 mM). Therefore, the results in this work provide an efficient and easily controlled method for industrial-scale production of α-arbutin.

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Acknowledgments

This project has been funded by the Key Projects in the National Science & Technology Pillar Program during the 12th 5 years Plan Period (2011BAD22B04), the National Natural Science Foundation of China (21246005), and the National Basic Research Program of China (973 program) (2013CB733600).

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Authors and Affiliations

  1. Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China

    Chunqiao Liu, Peng Zhang, Shurong Zhang, Tao Xu, Fang Wang & Li Deng

  2. Amoy-BUCT Industrial of Bio-technovation Institute, Amoy, 361022, China

    Li Deng

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  1. Chunqiao Liu
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  2. Peng Zhang
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  3. Shurong Zhang
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  4. Tao Xu
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  5. Fang Wang
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Correspondence to Chunqiao Liu.

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Chunqiao Liu, Peng Zhang contributed equally to this work.

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Liu, C., Zhang, P., Zhang, S. et al. Feeding strategies for the enhanced production of α-arbutin in the fed-batch fermentation of Xanthomonas maltophilia BT-112. Bioprocess Biosyst Eng 37, 325–329 (2014). https://doi.org/10.1007/s00449-013-0980-9

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