Abstract
Various selected strains from about 20 species of yeasts, which are reported to be sensitive to freeze-drying and liquid-drying, were successfully dried directly from the liquid phase without freezing using a simplified liquid-drying method. All tested cultures proved viable and the majority of the tested strains showed good survival rates after drying. However, different survival levels for different yeasts were observed; generally the sensitivity to drying appeared to be strain-specific. After 1 years' storage at 9°C, no further loss in viability was observed. Accelerated storage testing, for 1 week at 45°, resulted in further loss of viability to various degrees. Yeasts that were filamentous, osmotolerant or psychrophilic appeared to be sensitive to liquid-drying and had relatively lower survival levels than the others. Growth and liquid-drying under microaerobic conditions resulted in improved survival. The dried yeast cultures proved stable and no mutation or loss in desirable characters was detected. The method can be used for the drying and long-term preservation of nearly all yeast genera.
Similar content being viewed by others
References
Annear, D.I. 1962 Recoveries of bacteria after drying on cellulose fibres (a method for the routine preservation of bacteria). Australian Journal of Experimental Biology and Medical Sciences 40, 1–8.
Banno, T., Mikata, K. & Sakane, T. 1979 Viability of various yeasts after L-drying. Institute of Fermentation, Osaka, Research Communications 9, 27–34.
Banno, T. & Sakane, T. 1981 Prediction of prospective viability of L-dried cultures of bacteria after long-term preservation. Institute of Fermentation, Osaka, Research Communications 10, 33–38.
Hieda, K. 1981 Induction of genetic changes in Saccharomyces cerevisiae by partial drying in air of constant relative humidity and by UV. Mutation Research 84, 17–27.
Kirsop, B.E. 1974 The stability of biochemical, morphological and brewing properties of yeast cultures maintained by subculturing and freeze-drying. Journal of the Institute of Brewing 80, 565–570.
Kirsop, B.E. 1991 Maintenance of yeasts. In Maintenance of Microorganisms and Cultured Cells, eds Kirsop, B.E. & Doyle, A. pp. 161–182. London: Academic Press.
Kirsop, B.E. & Kurtzman, C.P. 1988 Yeasts. Living Resources for Biotechnology. Cambridge: Cambridge University Press.
Malik, K.A. 1976 Preservation of Knallgas bacteria. In Proceedings of 5th International Fermentation Symposium, ed. Dellweg, H. p. 180. Bonn & Berlin: Westkreuz.
Malik, K.A. 1988 A new freeze-drying method for the preservation of nitrogen-fixing and other fragile bacteria. Journal of Microbiological Methods 8, 259–279.
Malik, K.A. 1990a A simplified liquid-drying method for the preservation of microorganisms sensitive to freezing and freeze-drying. Journal of Microbiological Methods 12, 125–132.
Malik, K.A. 1990b Use of activated charcoal for the preservation of anaerobic phototrophic and other sensitive bacteria by freeze-drying. Journal of Microbiological Methods 12, 117–124.
Malik, K.A. 1991 Maintenance of microorganisms by simple methods. In Maintenance of Microorganisms and Cultured Cells, eds Kirsop, B.E. & Doyle, A. pp. 122–132. London: Academic Press.
Malik, K.A. 1992a A new method for preservation of microorganisms by liquid-drying under anaerobic conditions. Journal of Microbiological Methods 14, 239–245.
Malik, K.A. 1992b Some universal media for the isolation, growth and purity check of a broad spectrum of microorganisms. World Journal of Microbiology and Biotechnology 8, 453–456.
Mikata, K., Yamauchi, S. & Banno, I. 1983 Preservation of yeast cultures by L-drying: viabilities of 1710 yeasts after drying and storage. Institute of Fermentation, Osaka, Research Communications 11, 25–46.
Rodrigues de Miranda, L. & Yarrow, D. 1972 The maintenance of stock cultures of yeasts. In Proceedings of the 1st Specialized Symposium on Yeasts, eds Kockova-Kratochvilova, A. & Minarik, E. pp. 59–67.
Additional information
K.A. Malik and P. Hoffmann are with the DSM—Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, W-3300 Braunschweig, Germany
Rights and permissions
About this article
Cite this article
Malik, K.A., Hoffmann, P. Long-term preservation of yeast cultures by liquid-drying. World J Microbiol Biotechnol 9, 372–376 (1993). https://doi.org/10.1007/BF00383083
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00383083