Abstract
Paraffin sections of tissues fixed in absolute alcohol or Carnoy's fluid were mordanted in a 1% aqueous solution of phosphomolybdic acid, stained in saturated solutions of Sudan black B, acetylated Sudan black, various solvent and basic dyes in 70% ethyl alcohol for 5 min at room temperature, dehydrated in alcohol and covered in Permount. Sudan black B and other dyes with basic groups stained basement membranes, reticulum and collagen fibers intensely. Acetylated Sudan black, Sudan IV and oil red 0 did not color any tissue structures. Control sections, without pretreatment, did not bind Sudan black B. These findings indicate interaction between basic groups of the dye and free acid groups of phosphomolybdic acid.
Similar content being viewed by others
References
Byrne, J. M.: The uptake of dyes by extracted phospholipids and cerebrosides. Quart. J. micr. Sci. 103, 47–56 (1962).
Lillie, R. D., and H. J. Burtner: Stable sudanophila of human neutrophil leucocytes in relation to peroxidase and oxidase. J. Histochem. Cytochem. 1, 8–26 (1953).
Pratt, L. S.: The chemistry and physics of organic pigments. New York: John Wiley & Sons, Inc. 1947.
Puchtler, H., and H. Isler: The effect of phosphomolybdic acid on the stainability of connective tissues by various dyes. J. Histochem. Cytochem. 6, 265–270 (1958).
Schaeffer, A.: Chemikalienkunde für den Textilveredler. In: Handbuch der Färberei, Bd. V. Stuttgart: Konradin-Verlag Robert Kohlhammer 1951.
Terner, J. Y., J. Schnur, and J. Gurland: Stable sudanophilia. Contributions to the histochemistry of Sudan dyes. Lab. Invest. 12, 405–414 (1963).
Venkataraman, K.: The chemistry of synthetic dyes, vol. 2. New York: Academic Press Inc. 1952.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Puchtler, H., Sweat, F. Effect of phosphomolybdic acid on the binding of Sudan black B. Histochemie 4, 20–23 (1964). https://doi.org/10.1007/BF00304174
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00304174