Abstract
Filtration is one of the simplest forms of sample preparation prior to any analysis including mass spectrometry. Being the simplest, filtration is also one of the most neglected steps during optimization of sample preparation which can lead to problems in downstream analysis. This report provides guidelines towards selection of the right filtration device for the application. The factors which decide choice of filter are sample size, chemical characteristics of sample, particle load and downstream analytical technique. This report also provides useful information regarding extractables and analyte binding and ways to minimize both of these.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Brandt, I., Vriendt, K., Devreese, B., Beeumen, J., Dongen, W., Augustyns, K., Meester, I., Scharpé, S., and Lambeir, A. (2005). Search for substrates for prolyl oligopeptidase in porcine brain. Peptides 26, 2536–2546.
Chéneau, E., Henri, J., Pirotais, Y., Abjean, J., Roudaut, B., Sanders, P., and Laurentie, M. (2007). Liquid chromatography–electrospray tandem mass spectrometric method for quantification of monensin in plasma and edible tissues of chicken used in pharmacokinetic studies: Applying a total error approach. J Chromatogr B 850, 15–23.
González, A., Rodríguez-Velasco, M., Ben-Gigirey, B., and Botana, L. (2006). First evidence of spirolides in Spanish shellfish. Toxicon 48, 1068–1074.
Heuvel, R.H., and Heck, A.J. (2004). Native protein mass spectrometry: From intact oligomers to functional machineries. Curr Opin Chem Biol 8, 519–526.
Liu, D.Q., and Hop, C. (2005). Strategies for characterization of drug metabolites using liquid chromatography–tandem mass spectrometry in conjunction with chemical derivatization and on-line H/D exchange approaches. J Pharm Biomed Anal 37, 1–18.
Machtejevas, E., Andrecht, S., Lubda, D., and Unger, K. (2007). Monolithic silica columns of various format in automated sample clean-up/multidimensional liquid chromatography/mass spectrometry for peptidomics. J Chromatogr A 1144, 97–101.
Tsao, R., Yang, R., Young, J., Zhu, H., and Manolis, T. (2004). Separation of geometric isomers of native lutein diesters in marigold (Tagetes erecta L.) by high-performance liquid chromatography–mass spectrometry. J Chromatogr A 1045, 65–70.
Willems, A., Deforce, D., Lambert, W., Peteghem, C., and Bocxlaer, J. (2004). Rapid characterization of oligonucleotides by capillary liquid chromatography–nano electrospray quadrupole time-of-flight mass spectrometry. J Chromatogr A 1052, 93–101.
Wright, S., Pellegrino, J., and Amy, G. (2005). Humectant release from membrane materials. J Membr Sci 246, 227–234.
Yang, A., Sun, L., Musson, D., and Zhao, J. (2005). Application of a novel ultra-low elution volume 96-well solid-phase extraction method to the LC/MS/MS determination of simvastatin and simvastatin acid in human plasma. J Pharm Biomed Anal 38, 521–527.
Zurbonsen, K., Bressolle, F., Solassol, I., Aragon, P., Culine, S., and Pinguet, F. (2004). Simultaneous determination of dexamethasone and 6-hydroxydexamethasone in urine using solid-phase extraction and liquid chromatography: Applications to in vivo measurement of cytochrome P450 3A4 activity. J Chromatogr B 804, 421–429.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media B.V.
About this chapter
Cite this chapter
Joshi, V., Chernokalskaya, E. (2011). Filtration as a Sample Preparation Technique Prior to Mass Spectrometry: Selecting the Right Filtration Device. In: Ivanov, A., Lazarev, A. (eds) Sample Preparation in Biological Mass Spectrometry. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0828-0_4
Download citation
DOI: https://doi.org/10.1007/978-94-007-0828-0_4
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-007-0758-0
Online ISBN: 978-94-007-0828-0
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)