Abstract
Each of the major proteomics technology platforms, notably those that focus on peptides (“bottom-up” or “shotgun” MS strategies) and those that focus on intact proteins (2D gel-based, MALDI-imaging and “top-down” MS strategies) have unique strengths and limitations that in many cases can be complementary. These differences become even more evident in the realm of quantitative proteomics, where technical noise can play a large role in obscuring biological significance. This chapter focuses on the unique strengths that the 2D-gel based Difference Gel Electrophoresis (DIGE) quantitative approach can provide by enabling the interrogation of over 1000 resolved intact species from multiple biological replicates of multiple biological conditions with low technical noise and high statistical power. Examples will be highlighted where vital information on protein modifications or genomic variants were facilely obtained from global-scale analyses on intact protein forms.
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Friedman, D.B. (2011). Quantitative Intact Proteomic Strategies to Detect Changes in Protein Modification and Genomic Variation. In: Ivanov, A., Lazarev, A. (eds) Sample Preparation in Biological Mass Spectrometry. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0828-0_14
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DOI: https://doi.org/10.1007/978-94-007-0828-0_14
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