Zusammenfassung
Die Umgebungsbedingungen kultivierter Zellen in vitro umfassen weiterhin eine Reihe physiko-chemischer Parameter, die über die Kultivierungsdauer in engen, physiologischen Grenzen gehalten werden müssen. In vivo wird dies durch Regelmechanismen der Homöostase, der Konstanthaltung des inneren Milieus, bewerkstelligt. Neben einer konstanten Nährstoffversorgung und Temperatur, sind dies die
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Isoionie, die Konstanthaltung der ionalen Zusammensetzung des Extrazellulärraums, die
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Isotonie, die Konstanthaltung des osmotischen Drucks (extrazelluläre Osmolarität, Abschn. 8.1) und die
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Isohydrie, die Konstanthaltung der extrazellulären H+-Ionenkonzentration und damit des extrazellulären pH-Werts (Abschn. 8.4).
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Gstraunthaler, G., Lindl, T. (2013). Physiologische Zellkulturparameter. In: Zell- und Gewebekultur. Springer Spektrum, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35997-2_8
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