Chemically Inert Nanoparticles Affect Hemopoietic and Stromal Cells Microenvironments in Vitro

Abstract

Cellular and molecular processes developing in vitro during direct contact of chemically inert nanoparticles with mice HI and artificial niches for osteogenic differentiation of human stromal stem cells (SSC) were evaluated. Nano-sized carbon encapsulated iron powders Fe(C) (diameter <10 nm) and FeSi(C) (diameter < 20 nm) were prepared. Pure titanium discs (diameter 12 mm, thickness 1 mm) with bilateral CP coatings were used as the source of surface with artificial osteogenic niches. Chemical inertness of iron and composite nanoparticles with carbon covering was evident. Reactive oxygen species production caused by Fe(C) or FeSi(C) was insufficient to destroy cells and their microenvironment. However, low doses of chemically inert nanopowders were capable to diminish morphofunctional status and humoral cooperation of SSC with hemopoietic cells and CP coatings. For all this, different heat generation (Q) for Fe(C) and FeSi(C) specimens has been revealed. We proposed thermodynamic functioning of interfaces between both different stem cells and CP surfaces.