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Raman Imaging of Plant Cell Walls

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Confocal Raman Microscopy

Part of the book series: Springer Series in Optical Sciences ((SSOS,volume 158))

Abstract

To gain a better understanding of plant cell walls, several microscopic and chemical methods have been used for their analysis in recent years. However, a knowledge gap exists about the location, quantity, and structural arrangement of molecules on the micrometer scale within the native cell wall. Advances in confocal Raman microscopy and imaging have tackled this problem in a non-invasive way and provide chemical and structural information in situ with a high spatial resolution (< 0.5 μm).

Examples of high-resolution Raman imaging on wood cells are given, showing that changes in polymer chemistry and orientation can be followed within and between different cell wall layers. In horsetail (Equisetum hyemale [E. Hyemale]) tissue, in addition to the mapping of plant cell wall polymers, the distribution of amorphous silica is investigated. Area scans from a cross-section are included as well as a depth profiling within a silica-rich knob on the outer stem wall.

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Author information

Authors and Affiliations

  1. Department of Biomaterials, Max-Planck-Institute of Colloids and Interfaces, Am Mühlenberg 1, D- 14476, Potsdam-Golm, Germany

    Notburga Gierlinger

  2. Johannes Kepler University, Institute of Polymer Science, Altenbergerstraße 69, A-4040, Linz, Austria

    Notburga Gierlinger

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  1. Notburga Gierlinger
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Correspondence to Notburga Gierlinger .

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Editors and Affiliations

  1. Instrumente und Technologie GmbH (WITec), Wissenschaftliche, Hoervelsinger Weg 6, Ulm, 89081, Germany

    Thomas Dieing

  2. Instrumente und Technologie GmbH (WITec), Wissenschaftliche, Hoervelsinger Weg 6, Ulm, 89081, Germany

    Olaf Hollricher

  3. Instrumente und Technologie GmbH (WITec), Wissenschaftliche, Hoervelsinger Weg 6, Ulm, 89081, Germany

    Jan Toporski

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Gierlinger, N. (2010). Raman Imaging of Plant Cell Walls. In: Dieing, T., Hollricher, O., Toporski, J. (eds) Confocal Raman Microscopy. Springer Series in Optical Sciences, vol 158. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-12522-5_10

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  • DOI: https://doi.org/10.1007/978-3-642-12522-5_10

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-12521-8

  • Online ISBN: 978-3-642-12522-5

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