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High-Resolution Optical and Confocal Microscopy

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Confocal Raman Microscopy

Part of the book series: Springer Series in Optical Sciences ((SSOS,volume 158))

Abstract

In this chapter, the theory of optical image formation in an optical microscope is described, and the difference between conventional and confocal microscopy is explained. The selection of the appropriate pinhole diameter is discussed in detail, as the pinhole diameter is crucial in obtaining the highest depth resolution as well as optimizing collection efficiency, because the Raman signal is typically very weak.

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References

  1. J.B. Pawley, Handbook of Biological Confocal Microscopy, 2nd edn. (Plenum Press, New York, 1995)

    Google Scholar 

  2. E. Wolf, Proc. R. Soc. London A 253, 358 (1959)

    Article  MATH  ADS  Google Scholar 

  3. E. Wolf, Proc. Phys. Soc. 74, 269 (1959)

    Article  MATH  MathSciNet  ADS  Google Scholar 

  4. B. Richards, E. Wolf, Proc. R. Soc. London A 253, 358 (1959)

    Article  MATH  ADS  Google Scholar 

  5. R. Juškaitis, T. Wilson, J. Microsc. 189, 8 (January 1998)

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  6. T. Wilson, Confocal Microscopy, Chap. IV.2. The imaging of simple objects (Academic, 1990) pp. 25–30

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  7. T. Wilson, Confocal Microscopy, Chap. IV.2. The imaging of simple objects (Academic, 1990) pp. 41–45

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  8. T. Wilson, A.R. Carlini, Opt. Lett. 12, 227 (April 1987)

    Article  ADS  Google Scholar 

  9. C.J.R. Sheppard, M. Gu, Optik 86(3), 104 (1990)

    Google Scholar 

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Author information

Authors and Affiliations

  1. Research & Development, WITec GmbH, Lise-Meitner-Strasse 6, D-89081, Ulm, Germany

    Olaf Hollricher

  2. WITec GmbH, Lise-Meitner-Strasse 6, D-89081, Ulm, Germany

    Wolfram Ibach

Authors
  1. Olaf Hollricher
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  2. Wolfram Ibach
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Corresponding author

Correspondence to Olaf Hollricher .

Editor information

Editors and Affiliations

  1. Instrumente und Technologie GmbH (WITec), Wissenschaftliche, Hoervelsinger Weg 6, Ulm, 89081, Germany

    Thomas Dieing

  2. Instrumente und Technologie GmbH (WITec), Wissenschaftliche, Hoervelsinger Weg 6, Ulm, 89081, Germany

    Olaf Hollricher

  3. Instrumente und Technologie GmbH (WITec), Wissenschaftliche, Hoervelsinger Weg 6, Ulm, 89081, Germany

    Jan Toporski

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Hollricher, O., Ibach, W. (2010). High-Resolution Optical and Confocal Microscopy. In: Dieing, T., Hollricher, O., Toporski, J. (eds) Confocal Raman Microscopy. Springer Series in Optical Sciences, vol 158. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-12522-5_1

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  • DOI: https://doi.org/10.1007/978-3-642-12522-5_1

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-12521-8

  • Online ISBN: 978-3-642-12522-5

  • eBook Packages: Physics and AstronomyPhysics and Astronomy (R0)

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