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Centrifuge-Based Fluidic Platforms

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Springer Handbook of Nanotechnology

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Abstract

In this chapter centrifuge-based microfluidic platforms are reviewed and compared with other popular microfluidic propulsion methods. The underlying physical principles of centrifugal pumping in microfluidic systems are presented and the various centrifuge fluidic functions such as valving, decanting, calibration, mixing, metering, heating, sample splitting, and separation are introduced. Those fluidic functions have been combined with analytical measurements techniques such as optical imaging, absorbance and fluorescence spectroscopy and mass spectrometry to make the centrifugal platform a powerful solution for medical and clinical diagnostics and high-throughput screening (HTS) in drug discovery. Applications of a compact disc (CD)-based centrifuge platform analyzed in this review include: two-point calibration of an optode-based ion sensor, an automated immunoassay platform, multiple parallel screening assays and cellular-based assays. The use of modified commercial CD drives for high-resolution optical imaging is discussed as well. From a broader perspective, we compare the technical barriers involved in applying microfluidics for sensing and diagnostic as opposed to applying such techniques to HTS. The latter poses less challenges and explains why HTS products based on a CD fluidic platform are already commercially available, while we might have to wait longer to see commercial CD-based diagnostics.

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Abbreviations

AC:

alternating-current

AC:

amorphous carbon

BSA:

bovine serum albumin

CD:

compact disc

CD:

critical dimension

DC:

direct-current

DMSO:

dimethyl sulfoxide

DNA:

deoxyribonucleic acid

DVD:

digital versatile disc

EHD:

elastohydrodynamic

GFP:

green fluorescent protein

HTS:

high throughput screening

MALDI:

matrix assisted laser desorption ionization

MEMS:

microelectromechanical system

PC:

polycarbonate

PDMS:

polydimethylsiloxane

PMMA:

poly(methyl methacrylate)

RNA:

ribonucleic acid

RPC:

reverse phase column

RPM:

revolutions per minute

SEM:

scanning electron microscope

SEM:

scanning electron microscopy

SNP:

single nucleotide polymorphisms

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Author information

Authors and Affiliations

  1. Department of Math and Science, Saddleback College, 28000 Marguerite Parkway, 92692, Mission Viejo, CA, USA

    Jim V. Zoval

  2. Department of Mechanical and Aerospace Engineering, University of California, Irvine, Irvine, CA, USA

    Guangyao Jia

  3. Mechanical and Aerospace Engineering, University of California at Irvine, Irvine, CA, USA

    Horacio Kido

  4. Department of Mechanical and Aerospace Engineering, University of California at Irvine, Irvine, CA, USA

    Jitae Kim

  5. Research and Development, Samsung Advanced Institute of Technology, Seoul, South Korea

    Nahui Kim

  6. Department of Mechanical and Aerospace and Biomedical Engineering, University of California Irvine, Irvine, CA, USA

    Marc J. Madou

Authors
  1. Jim V. Zoval
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  2. Guangyao Jia
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  3. Horacio Kido
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  4. Jitae Kim
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  5. Nahui Kim
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  6. Marc J. Madou
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Corresponding authors

Correspondence to Jim V. Zoval , Guangyao Jia , Horacio Kido , Jitae Kim , Nahui Kim or Marc J. Madou .

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Editors and Affiliations

  1. Ohio State University, Nanoprobe Laboratory for Bio- and Nanotechnology and Biomimetics (NLB2), 201 W. 19th Avenue, 43210-1142, Columbus, OH, USA

    Bharat Bhushan

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Zoval, J.V., Jia, G., Kido, H., Kim, J., Kim, N., Madou, M.J. (2010). Centrifuge-Based Fluidic Platforms. In: Bhushan, B. (eds) Springer Handbook of Nanotechnology. Springer Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02525-9_19

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  • DOI: https://doi.org/10.1007/978-3-642-02525-9_19

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