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G-Protein Coupled Receptors: Progress in Surface Display and Biosensor Technology

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Springer Handbook of Nanotechnology

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Abstract

Signal transduction by G-protein coupled receptors (GPCRs) underpins a multitude of physiological processes. Ligand recognition by these receptors leads to activation of a generic molecular switch involving heterotrimeric G-proteins and guanine nucleotides. With growing interest and commercial investment in GPCRs in areas such as drug targets, orphan receptors, high-throughput screening of drugs, biosensors etc., greater attention will focus on assay development to allow for miniaturization, ultrahigh throughput, and eventually, microarray/biochip assay formats that will require nanotechnology-based approaches. Stable, robust, cell-free signaling assemblies comprising receptor and appropriate molecular switching components will form the basis of future GPCR/G-protein platforms which should be adaptable for such applications as microarrays and biosensors. This chapter focuses on cell-free GPCR assay nanotechnologies and describes some molecular biological approaches for the construction of more sophisticated, surface-immobilized, homogeneous, functional GPCR sensors. The latter points should greatly extend the range of applications to which technologies based on GPCRs could be applied.

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Abbreviations

AFM:

atomic force microscope

AFM:

atomic force microscopy

ATP:

adenosine triphosphate

FCS:

fluorescence correlation spectroscopy

FP:

fluorescence polarization

FPR:

N-formyl peptide receptor

GDP:

guanosine diphosphate

GFP:

green fluorescent protein

GPCR:

G-protein coupled receptor

GTP:

guanosine triphosphate

HTS:

high throughput screening

IMAC:

immobilized metal ion affinity chromatography

NTA:

nitrilotriacetate

PLC:

phospholipase C

PWR:

plasmon-waveguide resonance

SPR:

surface plasmon resonance

TCM:

tetracysteine motif

TIRF:

total internal reflection fluorescence

TMR:

tetramethylrhodamine

TPE-FCCS:

two-photon excitation fluorescence cross-correlation spectroscopy

YFP:

yellow fluorescent protein

cAMP:

cyclic adenosine monophosphate

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Author information

Authors and Affiliations

  1. CSIRO Human Nutrition, Adelaide Business Centre, 5000, Adelaide, SA, Australia

    Wayne R. Leifert Dr.

  2. CSIRO Human Nutrition, University of Adelaide, 5005, Adelaide, SA, Australia

    Tamara H. Cooper

  3. CSIRO Human Nutrition, University of Adelaide, 5005, Adelaide, SA, Australia

    Kelly Bailey

Authors
  1. Wayne R. Leifert Dr.
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  2. Tamara H. Cooper
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  3. Kelly Bailey
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Corresponding authors

Correspondence to Wayne R. Leifert Dr. , Tamara H. Cooper or Kelly Bailey .

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Editors and Affiliations

  1. Ohio State University, Nanoprobe Laboratory for Bio- and Nanotechnology and Biomimetics (NLB2), 201 W. 19th Avenue, 43210-1142, Columbus, OH, USA

    Bharat Bhushan

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Leifert, W.R., Cooper, T.H., Bailey, K. (2010). G-Protein Coupled Receptors: Progress in Surface Display and Biosensor Technology. In: Bhushan, B. (eds) Springer Handbook of Nanotechnology. Springer Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02525-9_17

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  • DOI: https://doi.org/10.1007/978-3-642-02525-9_17

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