Abstract
In this chapter, we discuss the incorporation of molecules into nanodevices as functional device components. Our primary focus is on biological molecules, although we also discuss the use of organic molecules as functional components of supramolecular nanodevices. Our primary device interest is in devices used in human therapy and diagnosis, though when it is informative, we discuss other nontherapeutic nanodevices containing biomolecular components. We discuss design challenges associated with devices built from prefabricated components (biological macromolecules) but that are not as frequently associated with fully synthetic nanodevices. Some design challenges (abstraction of device object properties, inputs, and outputs) can be addressed using existing systems engineering approaches and tools (including unified modeling language), whereas others (selection of optimal biological macromolecules from the billions available) have not been fully addressed. We discuss various assembly strategies applicable to biological macromolecules and organic molecules (self-assembly, chemoselective conjugation) and their advantages and disadvantages. We provide an example of a functional mesoscale device, a planar field-effect transistor (FET) protein sensor, that depends on nanoscale components for its function. We also use the sensor platform to illustrate how protein and other molecular engineering approaches can address nanoscale technological problems, and argue that protein engineering is a legitimate nanotechnology in this application. In developing the functional FET sensor, both direct adsorption of protein analyte receptors as well as linkage of receptors to the sensing surface through a polymer layer were tested. However, in the realized FET sensor, interfaces consist of a polymer layer linked to the semiconductor surface and to an analyte receptor (a protein). Nanotribology and other surface-science investigations of the interfaces revealed phenomena not previously documented for nanoscale protein interfaces (lubrication by directly adsorbed proteins, increases in friction force associated with polymer-mediated increases in sample compliance). Furthermore, the studies revealed wear of polymer and receptor proteins from semiconductor surfaces by an atomic force microscopy (AFM) tip which was not a concerted process, but rather depth of wear increased with increasing load on the cantilever. These studies also revealed that the polymer–protein interfaces were disturbed by nanonewton forces, suggesting that interfaces of immunoFET protein sensors translated to in vivo use must likely be protected from, or hardened to endure, abrasion from tissue. The results demonstrate that nanoscience (in this case, nanotribology) is needed to design and characterize functional planar immunoFET sensors, even though the sensors themselves are mesoscale devices. The results further suggest that modifications made to the sensor interfaces to address these nanoscale challenges may be best accomplished by protein and interfacial engineering approaches.
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Abbreviations
- 1-D:
-
one-dimensional
- 3-D:
-
three-dimensional
- AA:
-
amino acid
- AFM:
-
atomic force microscope
- AFM:
-
atomic force microscopy
- AMU:
-
atomic mass unit
- APDMES:
-
aminopropyldimethylethoxysilane
- APTES:
-
aminopropyltriethoxysilane
- CDR:
-
complementarity determining region
- CMC:
-
cell membrane complex
- CMC:
-
critical micelle concentration
- COG:
-
cost of goods
- CP:
-
circularly permuted
- DPN:
-
dip-pen nanolithography
- EDC:
-
1-ethyl-3-(3-diamethylaminopropyl) carbodiimide
- FET:
-
field-effect transistor
- IKVAV:
-
isoleucine–lysine–valine–alanine–valine
- MOSFET:
-
metal–oxide–semiconductor field-effect transistor
- MRI:
-
magnetic resonance imaging
- SCFv:
-
single-chain fragment variable
- SWCNT:
-
single-wall carbon nanotube
- SWCNT:
-
single-walled carbon nanotube
- UAA:
-
unnatural AA
- UML:
-
unified modeling language
- VHH:
-
variable heavy–heavy
- ds:
-
double-stranded
References
S.C. Lee, R. Parthasarathy, T. Duffin, K. Botwin, T. Beck, G. Lange, J. Zobel, D. Jansson, D. Kunneman, E. Rowold, C.F. Voliva: Antibodies to PAMAM dendrimers: Reagents for immune detection assembly and patterning of dendrimers. In: Dendrimers and Other Dendritic Polymers, ed. by D. Tomalia, J. Frechet (Wiley, London 2001) pp. 559–566
S.C. Lee: Biotechnology for nanotechnology, Trends Biotechnol. 16, 239–240 (1998)
S.C. Lee: Engineering the protein components of nanobiological devices. In: Biological Molecules in Nanotechnology: The Convergence of Biotechnology, Polymer Chemistry and Materials Science, ed. by S.C. Lee, L. Savage (IBC, Southborough 1998) pp. 67–74
S.C. Lee: How a molecular biologist can wind up organizing nanotechnology meetings. In: Biological Molecules in Nanotechnology: The Convergence of Biotechnology, Polymer Chemistry and Materials Science, ed. by S.C. Lee, L. Savage (IBC, Southborough 1998)
S.C. Lee: The nanobiological strategy for construction of nanodevices. In: Biological Molecules in Nanotechnology: The Convergence of Biotechnology, Polymer Chemistry and Materials Science, ed. by S.C. Lee, L. Savage (IBC, Southborough 1998) pp. 3–14
S.C. Lee: A biological nanodevice for drug delivery, National Science and Technology Council. IWGN Workshop Report: Nanotechnology Research Directions. International Technology Research Institute, World Technology Division (Kluwer, Baltimore 1999) pp. 91–92
S.C. Lee, R. Parthasarathy, K. Botwin: Proteinpolymer conjugates: Synthesis of simple nanobiotechnological devices, Polym. Prepr. 40, 449–450 (1999)
L. Jelinski: Biologically related aspects of nanoparticles, nanostructured materials and nanodevices. In: Nanostructure Science and Technology, ed. by R.W. Siegel, E. Hu, M.C. Roco (Kluwer, Dordrecht 1999) pp. 113–130
B.R. Smith, J. Heverhagen, M. Knopp, P. Schmalbrock, J. Shapiro, M. Shiomi, N. Moldovan, M. Ferrari, S.C. Lee: Magnetic resonance imaging of atherosclerosis in vivo using biochemically targeted ultrasmall superparamagnetic iron oxide particles (SPIONs), Biomed. Microdevices 9, 719–728 (2007)
A.J. Nijdam, T.R. Nicholson III, J. Shapiro, B.R. Smith, J.T. Heverhagen, P. Schmalbrock, M.V. Knopp, A. Kebbel, D. Wang, S.C. Lee: Biochemically targeted nanoparticulate contrast agents for magnetic resonance imaging diagnosis of cardiovascular disease, Curr. Nanosci. 5, 88–102 (2009)
J.R. Baker Jr.: Therapeutic nanodevices. In: Biological Molecules in Nanotechnology: The Convergence of Biotechnology, Polymer Chemistry and Materials Science, ed. by S.C. Lee, L. Savage (IBC, Southborough 1998) pp. 173–183
R. Duncan: Drug targeting: Where are we now and where are we heading?, J. Drug Target. 5, 1–4 (1997)
R. Duncan, S. Gac-Breton, R. Keane, Y.N. Sat, R. Satchi, F. Searle: Polymer-drug conjugates, PDEPT and PELT: Basic principles for design and transfer from the laboratory to clinic, J. Cont. Release 74, 135–146 (2001)
D.S. Goldin, C.A. Dahl, K.L. Olsen, L.H. Ostrach, R.D. Klausner: Biomedicine. The NASA-NCI collaboration on biomolecular sensors, Science 292, 443–444 (2001)
S.C. Lee: Dendrimers in nanobiological devices. In: Dendrimers and Other Dendritic Polymers, ed. by D. Tomalia, J. Frechet (Wiley, London 2001) pp. 548–557
J.R. Baker Jr., A. Quintana, L. Piehler, M. Banazak-Holl, D. Tomalia, E. Racka: The synthesis and testing of anti-cancer therapeutic nanodevices, Biomed. Microdevices 3, 61–69 (2001)
K.D. Bhalerao, E. Eteshola, M. Keener, S.C. Lee: Nanodevice design through the functional abstraction of biological macromolecules, Appl. Phys. Lett. 87, 143902–143904 (2005)
S.C. Lee, K. Bhalerao, M. Ferrari: Object oriented design tools for supramolecular devices and biomedical nanotechnology, Ann. New York Acad. Sci. 1013, 110–123 (2004)
J.M. Harris, N.E. Martin, M. Modi: Pegylation: A novel process for modifying pharmacokinetics, Clin. Pharmacokin. 40, 539–551 (2001)
S.B.H. Kent: Building proteins through chemistry: Total chemical synthesis of protein molecules by chemical ligation of unprotected protein segments. In: Biological Molecules in Nanotechnology: The Convergence of Biotechnology, Polymer Chemistry and Materials Science, ed. by S.C. Lee, L. Savage (IBC, Southborough 1998) pp. 75–92
C.A. Janeway, P. Travers, M. Walport, J.D. Capra: Immunobiology (Elsevier, London 1999)
S.C. Lee, M.S. Leusch, V.A. Luckow, P. Olins: Method of producing recombinant viruses in bacteria, US Patent 5348886 (1993)
M.S. Leusch, S.C. Lee, P.O. Olins: A novel hostvector system for direct selection of recombinant baculoviruses (bacmids) in E. coli, Gene 160, 191–194 (1995)
V.A. Luckow, S.C. Lee, G.F. Barry, P.O. Olins: Efficient generation of infectious recombinant baculoviruses by site-specific, transposon-mediated insertion of foreign DNA into a baculovirus genome propagated in E. coli, J. Virol. 67, 4566–4579 (1993)
T. Gardner, C.R. Cantor, J.J. Collins: Construction of a genetic toggle switch in E. coli, Nature 403, 339–342 (2000)
J. Hasty, F. Isaacs, M. Dolnik, D. McMillen, J.J. Collins: Designer gene networks: Towards fundamental cellular control, Chaos 11, 107–220 (2001)
S. Uppuluri, D.R. Swanson, L.T. Piehler, J. Li, G. Hagnauer, D.A. Tomalia: Core shell tecto(dendrimers). I. Synthesis and characterization of saturated shell models, Adv. Mater. 12, 796–800 (2000)
A.K. Patri, I.J. Majoros, J.R. Baker Jr.: Dendritic polymer macromolecular carriers for drug delivery, Curr. Opin. Chem. Biol. 6, 466–471 (2002)
A. Quintana, E. Raczka, L. Piehler, I. Lee, A. Myc, I. Majoros, A.K. Patri, T. Thomas, J. Mule, J.R. Baker Jr.: Design and function of a dendrimer-based therapeutic nanodevice targeted to tumor cells through the folate receptor, Pharma. Res. 19, 1310–1316 (2002)
Y. Choi, T. Thomas, A. Kotlyar, M. Islam, J. Baker Jr.: Synthesis and functional evaluation of DNA-assembled polyamidoamine dendrimer clusters for cancer cell-specific targeting, Chem. Biol. 12, 35–43 (2005)
Y. Choi, A. Mecke, B.G. Orr, M.M. Banaszak Holl, J.R. Baker Jr.: DNA-directed synthesis of generation 7 and 5 PAMAM dendrimer nanoclusters, Nano Lett. 4, 391–397 (2004)
D.G. Mullen, A.M. Desai, J.N. Waddell, X.-M. Cheng, C.V. Kelly, D.Q. McNerny, I.J. Majoros, J.R. Baker Jr., L.M. Sander, B.G. Orr, M.M. Banaszak Holl: The implications of stochastic synthesis for the conjugation of functional groups to nanoparticles, Bioconjug. Chem. 19, 1748–1752 (2008)
T.R. Groves, D. Pickard, B. Rafferty, N. Crosland, D. Adam, G. Schubert: Maskless electron beam lithography: Propects, progress and challenges, Microelectron. Eng. 61, 285–293 (2002)
M. Guthold, R. Superfine, R. Taylor: The rules are changing: Force measurements on single molecules and how they relate to bulk reaction kinetics and energies, Biomed. Microdevices 3, 9–18 (2001)
L.M. Demers, D.S. Ginger, S.-J. Park, Z. Li, S.-W. Chung, C.A. Mirkin: Direct patterning of modified oligonucleotides on metals and insulatos by dip-pen nanolithography, Science 296, 1836–1838 (2002)
K.-B. Lee, S.-J. Park, C.A. Mirkin, J.C. Smith, M. Mrksich: Protein nanoarrays generated by dippen nanolithography, Science 295, 1702–1705 (2002)
M. Ferrari, J. Liu: The engineered course of treatment, Mech. Eng. 123, 44–47 (2001)
K.E. Drexler: Engines of Creation: The Coming Era of Nanotechnology (Anchor Books, New York 1986)
J. Cumings, A. Zetti: Low-friction nanoscale linear bearing realized frommultiwall carbon nanotubes, Science 289, 602–604 (2000)
D.J. Hornbaker, S.-J. Kahng, S. Mirsa, B.W. Smith, A.T. Johnson, E.J. Mele, D.E. Luzzi, A. Yazdoni: Mapping the one-dimensional electronic states of nanotube peapod structures, Science 295, 828–831 (2002)
C. Dekker: Carbon nanotubes as molecular quantum wires, Phys. Today 28, 22–28 (1999)
M.-C. Jones, J.-C. Leroux: Polymeric micellesa new generation of colloidal drug carriers, Eur. J. Pharma. Biopharma. 48, 101–111 (1999)
I. Uchegbu: Parenteral drug delivery: 1, Pharma. J. 263, 309–318 (1999)
I. Uchegbu: Parenteral drug delivery: 2, Pharma. J. 263, 355–359 (1999)
J.D. Hartgerink, E.R. Zubarev, S.I. Stupp: Supramolecular one-dimensional objects, Curr. Opin. Solid State Mater. Sci. 5, 355–361 (2001)
L.C. Palmer, Y.S. Velichko, M. Olvera De La Cruz, S.I. Stupp: Supramolecular self-assembly codes for functional structures, Philos. Trans. R. Soc. A 365, 1417–1433 (2007)
G.A. Silva, C. Catherine, K.L. Niece, E. Beniash, D.A. Harrington, J.A. Kessler, S.I. Stupp: Selective differentiation of neural progenitor cells by high-epitope density nanofibers, Science 303, 1352–1355 (2004)
V.M. Tysseling-Mattiace, V. Sahni, K.L. Niece, D. Birch, C. Czeisler, M. Fehlings, S.I. Stupp, J.A. Kessler: Self-assembling nanofibers inhibit glial scar gormation and promote axon elongation after spinal cord injury, J. Neurosci. 28, 3814–3823 (2008)
S. Fernandez-Lopez, H.-S. Kim, E.C. Choi, M. Delgado, J.R. Granja, A. Khasanov, K. Kraehenbuehl, G. Long, D.A. Weinberger, K.M. Wilcoxen, M. Ghardiri: Antibacterial agents based on the cyclic D,L-alpha-peptide architecture, Nature 412, 452–455 (2001)
A. Saghatelian, Y. Yokobayashi, K. Soltani, M.R. Ghadiri: A chiroselective peptide replicator, Nature 409, 777–778 (2001)
T. Hamouda, A. Myc, B. Donovan, A.Y. Shih, J.D. Reuter, J.R. Baker Jr.: A novel surfactant nanoemulsion with a unique non-irritant topical antimicrobial activity against bacteria, enveloped viruses and fungi, Microbiol. Res. 156, 1–7 (2001)
J. Davies: Aminoglycoside-aminocyclitol antibiotics and their modifying enzymes. In: Antibiotics in Laboratory Medicine, ed. by V. Lorian (Williams and Wilkins, Baltimore 1984) pp. 474–489
M.J. Heller: Utilization of synthetic DNA for molecular electronic and photonic-based device applications. In: Biological Molecules in Nanotechnology: The Convergence of Biotechnology, Polymer Chemistry and Materials Science, ed. by S.C. Lee, L. Savage (IBC, Southborough 1998) pp. 59–66
Z. Ma, S. Taylor: Nucleic acid triggered catalytic drug release, Proc. Natl. Acad. Sci. USA 97, 11159–11163 (2000)
R.C. Merkle: Biotechnology as a route to nanotechnology, Trends Biotechnol. 17, 271–274 (1999)
N.C. Seeman, J. Chen, Z. Zhang, B. Lu, H. Qiu, T.-J. Fu, Y. Wang, X. Li, J. Qi, F. Liu, L.A. Wenzler, S. Du, J.E. Mueller, H. Wang, C. Mao, W. Sun, Z. Shen, M.H. Wong, R. Sha: A bottom-up approach to nanotechnology using DNA. In: Biological Molecules in Nanotechnology: The Convergence of Biotechnology, Polymer Chemistry and Materials Science, ed. by S.C. Lee, L. Savage (IBC, Southborough 1998) pp. 45–58
G. Lemieux, C. Bertozzi: Chemoselective ligation reactions with proteins, oligosaccharides and cells, Trends Biotechnol. 16, 506–512 (1998)
R. Offord, K. Rose: Multicomponent synthetic constructs. In: Biological Molecules in Nanotechnology: The Convergence of Biotechnology, Polymer Chemistry and Materials Science, ed. by S.C. Lee, L. Savage (IBC, Southborough 1998) pp. 93–105
S.C. Lee, R. Parthasarathy, K. Botwin, D. Kunneman, E. Rowold, G. Lange, J. Zobel, T. Beck, T. Miller, W. Hood, J. Monahan, R. Jansson, J.P. McKearn, C.F. Voliva: Biochemical and immunological properties of cytokines conjugated to dendritic polymers, Biomed. Microdevices Biomems Biomed. Nanotechnol. 6, 191–201 (2004)
S.C. Lee, R. Parthasarathy, T. Duffin, K. Botwin, T. Beck, G. Lange, J. Zobel, D. Kunneman, E. Rowold, C.F. Voliva: Recognition properties of antibodies to PAMAM dendrimers and their use in immune detection of dendrimers, Biomed. Microdevices 3, 51–57 (2001)
G.T. Hermanson: Bioconjugate Chemistry (Academic, San Diego 1996)
S. Topell, R. Glockshuber: Circular permutation of the green fluorescent protein, Meth. Mol. Biol. 183, 31–48 (2002)
A. Rojas, S. Garcia-Vallve, J. Palau, A. Romeu: Circular permutations in proteins, Biologia 54, 255–277 (1999)
T.U. Schwartz, R. Walczak, G. Blobel: Circular permutation as a tool to reduce surface entropy triggers crystallization of the signal recognition particle receptor beta subunit, Protein Sci. 13, 2814–2818 (2004)
U. Heinemann, M. Hahn: Circular permutation of polypeptide chains: Implications for protein folding and stability, Prog. Biophys. Mol. Biol. 64, 121–143 (1996)
A. Buchwalder, H. Szadkoski, K. Kirschner: A fully active variant of dihydrofolate reductase with a circularly permuted sequence, Biochem. 31, 1621–1630 (1992)
L.S. Mullins, K. Wesseling, J.M. Kuo, J.B. Garrett, F.M. Raushel: Transposition of protein sequences: Circular permutation of ribonuclease T1, J. Am. Chem. Soc. 116, 5529–5533 (1994)
M. Hahn, K. Piotukh, R. Borriss, U. Heinemann: Native-like in vivo folding of a circularly permuted jellyroll protein shown by crystal structure analysis, Proc. Natl. Acad. Sci. USA 91, 10417–10421 (1994)
Y.R. Yang, H.K. Schachznan: Aspartate transcarbamoylase containing circularly permuted catalytic polypeptide chains, Proc. Natl. Acad. Sci. USA 90, 11980–11984 (1993)
X. Lin, G. Koelsch, J.A. Loy, J. Tang: Rearranging the domains of pepsinogen, Protein Sci. 4, 159–166 (1995)
M.L. Vignais, C. Corbier, G. Mulliert, C. Branlant, G. Branlant: Circular permutation within the coenzyme binding domain of the tetrameric glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus, Protein Sci. 4, 994–1000 (1995)
E. Eteshola, C.D. Van Valkenburgh, S. Merlin, E. Rowold, J. Adams, R. Ibdah, L.E. Pegg, A. Donelly, E. Rowold, J. Klover, S.C. Lee: Screening of a library of circularly permuted proteins on phage to manipulate protein topography, J. Nanoeng. Nanosyst. 219, 45–55 (2006)
E. Eteshola, M.T. Keener, M.A. Elias, J. Shapiro, L.J. Brillson, B. Bhushan, S.C. Lee: Engineering functional protein interfaces for immunologically modified field effect transistors (ImmunoFETs) by molecular genetic means, J. R. Soc. Interface 5, 123–127 (2008)
E. Eteshola, L. Brillson, S.C. Lee: Selection and characteristics of peptides that bind thermally grown silicon dioxide films, Biomol. Eng. 22, 202–204 (2005)
R.R. Naik, L.L. Brott, S.J. Clarson, M.O. Stone: Silica-precipitating peptides isolated from a combinatorial phage display peptide library, J. Nanosci. Nanotechnol. 2, 95–100 (2002)
E.M. Krauland, B.R. Peelle, K.D. Wittrup, A.M. Belcher: Peptide tags for enhanced cellular and protein adhesion to single-crystalline sapphire, Biotechnol. Bioeng. 97, 1009–1020 (2007)
L. Wang, J. Xie, P.G. Schultz: Expanding the genetic code, Annu. Rev. Biophys. Biomol. Struct. 35, 225–249 (2006)
J. Xie, P.G. Schultz: A chemical toolkit for proteins – an expanded genetic code, Nat. Rev. Mol. Cell Biol. 7, 775–782 (2006)
N. Hino, A. Hayashi, K. Sakamoto, S. Yokoyama: Site-specific incorporation of non-natural amino acids into proteins in mammalian cells with an expanded genetic code, Nat. Protoc. 1, 2957–2962 (2006)
K. Rogers: Principles of affinity-based biosensors, Mol. Biotechnol. 14, 109–129 (2000)
M.J. Schoning, A. Poghossian: Recent advances in biologically sensitive field-effect transistors (BioFETS), Analyst 127, 1137–1151 (2002)
P. Bergveld, J. Hendrikes, W. Olthuis: Theory and application of the material work function for chemical sensors based on the field effect principle, Meas. Sci. Technol. 9, 1801–1808 (1998)
W. Olthius, P. Bergveld, J. Kruise: The exploitation of ISFETs to determine acid-base behavior of proteins, Electrochim. Acta 43, 3483–3488 (1997)
R.B. Schasfoort, R.P. Kooyman, P. Bergveld, J. Greve: A new approach to ImmunoFET operation, Biosens. Bioelectron. 5, 103–124 (1990)
Y. Cui, Q. Wei, H. Park, C.M. Lieber: Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species, Science 293, 1289 (2001)
P. Bergveld: A critical evaluation of direct electrical protein detection methods, Biosens. Bioelectron. 6, 55–72 (1991)
J.I. Hahm, C.M. Lieber: Direct ultrasensitive electrical detection of DNA and DNA sequence variations using nanowire nanosensors, Nano Lett. 4, 51–54 (2004)
A. Star, J.C.P. Gabriel, K. Bradley, G. Gruner: Electronic detection of specific protein binding using nanotube FET devices, Nano Lett. 3, 459–463 (2003)
G. Zheng, F. Patolsky, Y. Cui, W.U. Wang, C.M. Lieber: Multiplexed electrical detection of cancer markers with nanowire sensor arrays, Nat. Biotechnol. 23, 1294–1301 (2005)
J. Shapiro, S. Gupta, E. Eteshola, M. Elias, X. Wen, W. Lu, L.J. Brillson, S.C. Lee: Challenges in optimization of nanobiotechnological devices illustrated by partial optimization of a protein biosensor, Proc. 2nd Int. Congr. Nanobiotechnol. Nanomed., NanoBio 2007 (Int. Association Nanotechnology, San Jose 2007), (CD)
S. Gupta, M. Elias, X. Wen, J. Shapiro, L. Brillson, W. Lu, S.C. Lee: Detection of clinically relevant levels of biological analyte under physiologic buffer using planar field effect transistors, Biosens. Bioelectron. 24, 505–511 (2008)
K. Decanniere, A. Desmyter, M. Lauwereys, M.A. Ghahroudi, S. Muyldermans, L. Wyns: A single-domain antibody fragment in complex with rnase a: non-canonical loop structures and nanomolar affinity using two CDR loops, Struct. Fold Des. 7, 361–370 (1999)
K. Decanniere, T.R. Transue, A. Desmyter, D. Maes, S. Muyldermans, L. Wyns: Degenerate interfaces in antigen-antibody complexes, J. Mol. Biol. 313, 473–478 (2001)
A. Desmyter, K. Decanniere, S. Muyldermans, L. Wyns: Antigen specificity and high affinity binding provided by one single loop of a camel single-domain antibody, J. Biol. Chem. 276, 26285–26290 (2001)
A. Muruganandam, J. Tanha, S. Narang, D. Stanimirovic: Selection of phage-displayed llama single-domain antibodies that transmigrate across human blood-brain barrier endothelium, FASEB Journal 16, 240–242 (2002)
S. Muyldermans: Single domain camel antibodies: Current status, Mol. Biotechnol. 74, 277–302 (2001)
S. Muyldermans, M. Lauwereys: Unique single-domain antigen binding fragments derived from naturally occurring camel heavy-chain antibodies, J. Mol. Recogn. 12, 131–140 (1999)
L. Riechmann, S. Muyldermans: Single domain antibodies: comparison of camel VH and camelised human VH domains, J. Immunol. Meth. 231, 25–38 (1999)
M.S. Hayden, L.K. Gilliland, J.A. Ledbetter: Antibody engineering, Curr. Opin. Immunol. 9, 201–212 (1997)
B. Bhushan, K.J. Kwak, S. Gupta, S.C. Lee: Nanoscale adhesion, friction and wear studies of biomolecules on polymer-coated silica and alumina based surfaces, J. R. Soc. Interface 6, 719–733 (2009)
Y. Han, D. Mayer, A. Offenhausser, S. Ingebrandt: Surface activation of thin silicon oxides by wet cleaning and silanization, Thin Solid Films 510, 175–180 (2006)
K. Kallury, P.M. MacDonald, M. Thompson: Effect of surface water and base catalysis on the silanization of silica by (aminopropyl)alkoxysilanes studied by x-ray photoelectron spectroscopy and 13C cross-polarization/magic angle spinning nuclear magnetic resonance, Langmuir 10, 492–499 (1994)
J.H. Moon, J.W. Shin, S.Y. Kim, J.W. Park: Formation of uniform aminosilane thin layers: An imine formation to measure relative surface density of the amine group, Langmuir 12, 4621–4624 (1996)
B. Bhushan, D.R. Tokachichu, M.T. Keener, S.C. Lee: Morphology and adhesion of biomolecules on silicon based surfaces, Acta Biomater. 1, 327–341 (2005)
S.C. Lee, M.T. Keener, D.R. Tokachichu, B. Bhushan, P.D. Barnes, B.J. Cipriany, M. Gao, L.J. Brillson: Protein binding on thermally grown silicon dioxide, J. Vac. Sci. Technol. B 23, 1856–1865 (2005)
B. Bhushan, D.R. Tokachichu, M.T. Keener, S.C. Lee: Nanoscale adhesion, friction and wear studies on silicon based surfaces, Acta Biomater. 2, 39–49 (2006)
S.C. Lee, M. Reugsegger, P.D. Barnes, B.R. Smith, M. Ferrari: Therapeutic nanodevices. In: Springer Handbook of Nanotechnology, ed. by B. Bhushan (Springer, Berlin, Heidelberg 2007) pp. 461–504
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Lee, S.C., Bhushan, B. (2010). Biological Molecules in Therapeutic Nanodevices. In: Bhushan, B. (eds) Springer Handbook of Nanotechnology. Springer Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02525-9_16
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