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X-Ray Microscopy II pp 343–348Cite as

X-Ray Contact Microscopy, Using Synchrotron and Laser Sources, of Ultrasectioned, Heavy Metal Contaminated Earthworm Tissue

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Part of the book series: Springer Series in Optical Sciences ((SSOS,volume 56))

Abstract

The technique of contact microscopy using soft X-rays is now established /1,2,3/ and enables a 1:1 image of the specimen to be formed in an X-ray sensitive resist which, after development, can be viewed by electron microscopy. The quality of the image so obtained depends on several things:- sensitivity of the resist; intimate contact of the specimen with the resist; exposure and development times; thickness/thinness of the sample. Because of the preferential absorption of X-rays by features within biological specimens, the degree of information gained will depend on the structural heterogeneity within the material. The technique also has the potential of imaging mineral deposits within a tissue, providing that the element has a high absorption cross section in the X-ray band used.

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Author information

Authors and Affiliations

  1. Department of Biological Science, University of Keele, Keele, Staffs., ST5 5BG, UK

    K. S. Richards

  2. Rutherford Appleton Laboratory, Didcot, Oxon., OX11 OQX, UK

    A. D. Rush

  3. Daresbury Laboratory, Warrington, Ches., WA4 4AD, UK

    D. T. Clarke & W. J. Myring

Authors
  1. K. S. Richards
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  2. A. D. Rush
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  3. D. T. Clarke
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  4. W. J. Myring
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Editor information

Editors and Affiliations

  1. IBM T.J. Watson Research Center, 10598, Yorktown Heights, NY, USA

    David Sayre Ph. D.

  2. Department of Physics, State University of New York, 11794, Stony Brook, NY, USA

    Janos Kirz Ph. D.

  3. Center for X-Ray Optics, Lawrence Berkeley Laboratory, University of California at Berkeley, 1 Cyclotron Rd., 94720, Berkeley, CA, USA

    Malcolm Howells Ph. D.

  4. National Synchrotron Light Source, Brookhaven National Laboratory, 11973, Upton, NY, USA

    Harvey Rarback Ph. D.

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© 1988 Springer-Verlag Berlin Heidelberg

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Richards, K.S., Rush, A.D., Clarke, D.T., Myring, W.J. (1988). X-Ray Contact Microscopy, Using Synchrotron and Laser Sources, of Ultrasectioned, Heavy Metal Contaminated Earthworm Tissue. In: Sayre, D., Kirz, J., Howells, M., Rarback, H. (eds) X-Ray Microscopy II. Springer Series in Optical Sciences, vol 56. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-39246-0_61

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  • DOI: https://doi.org/10.1007/978-3-540-39246-0_61

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-14490-9

  • Online ISBN: 978-3-540-39246-0

  • eBook Packages: Springer Book Archive

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