Abstract
Pancreatic cancer is a particularly lethal malignancy and is highly chemoresistant. There is an urgent need for the identification of new therapeutic targets and more effective treatment options. New approaches, such as high-throughput RNAi, enable the functional evaluation of the casual role of numerous genes in regulating cellular processes, such as cell survival and drug response. In the following chapter, we review RNA interference and its application in high-throughput biology. Specifically, an overview is provided highlighting important experimental aspects in transitioning RNAi to a high-throughput platform. In addition, there is a brief review of current applications of high-throughput RNAi for cancer target identification and drug discovery. Lastly, particular applications of genome-scale RNAi to pancreatic cancer target and treatment identification are discussed. In summary, genome-scale RNAi is proving to be a powerful cellular genomics technology that holds great promise for advancing pharmacologically relevant targets and agents in pancreatic cancer research.
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Yin, H., Kiefer, J., Kassner, M., Tang, N., Mousses, S. (2010). The Application of High-Throughput RNAi in Pancreatic Cancer Target Discovery and Drug Development. In: Han, H., Grippo, P. (eds) Drug Discovery in Pancreatic Cancer. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1160-5_8
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DOI: https://doi.org/10.1007/978-1-4419-1160-5_8
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