Androgenesis is a process of redirection of normal pollen development towards the formation of haploid embryos and ultimately doubled-haploid plants. This review gives a historical overview of different procedures used to induce androgenesis in tobacco. The various factors that influence this developmental switch including growth of donor plants, pre-treatments, culture media, as well as cytological aspects of cell and tissue changes that occur during the induction and development of the microspore-derived embryos are described. Finally, this review will cover recent molecular data on mechanisms of embryogenic induction in cultured tobacco microspores
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References
Aionesei T, Touraev A, Heberle-Bors E (2005) Pathways to microspore embryogenesis. In: Palmer CE, Keller WA, Kasha KJ (eds) Haploids in Crop Improvement II. Springer, Berlin/Heidelberg/ New York, pp 11–34
Anagnostakis SL (1974) Haploid plants from tobacco—Enhancement with charcoal. Planta 115: 281–283
Benito-Moreno RM, Macke F, Hauser M-T, Alwen A, Heberle-Bors E (1988) Sporophytes and male gametophytes from in vitro cultured immature tobacco pollen. In: Cresti M, Jori P, Pacini E (eds) Sexual Reproduction in Higher Plants. Springer, Berlin/Heidelberg/New York, pp 137–142
Binarova P, Straatman K, Hause B, Hause G, Van Lammeren AAM (1993) Nuclear DNA synthesis during the induction of embryogenesis in cultured microspores and pollen of Brassica napus L. Theor Appl Genet 87: 9–16
Bourgin JP, Nitsch JP (1967) Obtention de Nicotiana haploids à partir détamines cultivées in vitro. Ann Physiol 9: 377–382
Boutilier K, Fiers M, Liu C-M, Geest AHM (2005) Biochemical and molecular aspects of haploid embryogenesis, In: Palmer D, Keller W, Kasha K (eds) Haploids in Crop Improvement II. Springer, Heidelberg, pp 73–96
Clausen RE, Mann MC (1924) Inheritance in Nicotiana tabacum V. The occurrence of haploid plants in interspecific progenies. Proc Natl Acad Sci USA 10: 121–124
Duncan EJ, Heberle E (1976) Effect of temperature shock on nuclear phenomena in microspores of Nicotiana tabacum and consequently on plantlet production. Protoplasma 90: 173–177
Dunwell JM (1976) A comparative study of environmental and developmental factors which influence embryo induction and growth in cultured anthers of Nicotiana tabacum. Environ Exp Bot 16: 109–118
Dunwell JM (1981) Stimulation of pollen embryo induction in tobacco by pretreatment of excised anthers in a water-saturated atmosphere. Plant Sci Lett 21: 9–13
Dunwell JM, Sunderland N (1974a) Pollen ultrastructure in anther cultures of Nicotiana tabacum I. Early stages of culture. J Exp Bot 25: 352–361
Dunwell JM, Sunderland N (1974b) Pollen ultrastructure in anther cultures of Nicotiana tabacum II. Changes associated with embryogenesis. J Exp Bot 25: 363–373
Dunwell JM, Sunderland N (1975) Pollen ultrastructure in anther cultures of Nicotiana tabacum III. The first sporophytic division. J Exp Bot 26: 240–252
Dunwell JM, Sunderland N (1976) Pollen ultrastructure in anther cultures of Datura innoxia. J Cell Sci 22: 469–501
Garrido D, Charvat B, Benito-Moreno RM, Alwen A, Vicente O, Heberle-Bors E (1991) Pollen culture for haploid plant production in tobacco. In: Negrutiu I, Gharti-Chhetri G (eds) A Laboratory Guide for Cellular Molecular Plant Biology. Birkhäuser, Basel, pp 59–69
Garrido D, Eller N, Heberle-Bors E, Vicente O (1993) De novo transcription of specific messenger RNAs during the induction of tobacco pollen embryogenesis. Sex Plant Reprod 6: 40–45
Garrido D, Vicente O, Heberle-Bors E, Rodriguez-García MI (1995) Cellular changes during the acquisition of embryogenic potential in isolated pollen grains of Nicotiana tabacum. Protoplasma 186: 220–230
Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204: 497
Guha S, Maheshwari SC (1966) Cell division differentiation of embryos in the pollen grains of Datura in vitro. Nature 212: 97–98
Harada H, Kyo M, Imamura J (1988) The induction of embryogenesis in Nicotiana immature pollen in culture. In: Applications of plant cell tissue culture. Ciba Foundation Symposium, Wiley, Chichester, pp 59–74
Heberle-Bors E (1982) On the time of embryogenic pollen grain induction during sexual development of Nicotiana tabacum L plants. Planta 156: 402–406
Heberle-Bors E (1983) Induction of embryogenic pollen grains and sebsequent embryogenesis in Nicotiana tabacum L by treatments of the pollen donor plants with feminizing agents Physiol Plant 59: 67–72
Heberle-Bors E, Reinert J (1979) Androgenesis in isolated pollen cultures of Nicotiana tabacum: dependence upon pollen development. Protoplasma 99: 339–347
Heberle-Bors E, Reinert J (1980) Isolated pollen cultures and pollen dimorphism. Naturwiss 67: 311
Heberle-Bors E, Reinert J (1981) Environmental control and evidence for predetermination of pollen embryogenesis in Nicotiana tabacum pollen. Protoplasma 109: 249–255
Horner M, Street E (1978) Pollen dimorphism—origin and significance in pollen plant formation by anther culture. Ann Bot 42: 763–777
Horner M, McComb JA, McComb AJ, Street HE (1977) Ethylene production and plantlet formation by Nicotiana anthers cultured in the presence and absence of charcoal. J Exp Bot 28: 1365–1372
Hosp J, Tashpulatov A, Roessner U, Barsova E, Katholnigg H, Steinborn R, Melikant B, Lukyanov S, Heberle-Bors E, Touraev A (2007a) Transcriptional and metabolic profiles of stress- induced, embryogenic tobacco microspores. Plant Mol Biol 63: 137–149
Hosp J, Maraschin SF, Touraev A, Boutilier K (2007b) Functional genomics of microspore embryogenesis. Euphytica 158: 275–285
Imamura J, Harada H (1981) Stimulation of tobacco pollen embryogenesis by anaerobic treatments. Z Planzenphysiol 103: 259–263
Imamura J, Okabe E, Kyo M, Harada H (1982) Embryogenesis and plantlet formation through direct culture of isolated pollen of Nicotiana tabacum cv Samsum and Nicotiana rustica cv Rustica. Plant Cell Physiol 23: 713–716
Kohlenbach HW, Wernicke W (1978) Investigations on the inhibitory effect of agar and the function of active carbon in anther culture. Z Planzenphysiol 86: 463–472
Konar RN (1963) A haploid tissue from the pollen of Ephedra foliate. Boiss Phytomorphology 13: 170–174
Kyo M, Harada H (1985) Studies on conditions for cell division and embryogenesis in isolated pollen culture of Nicotiana rustica. Plant Physiol 79: 90–94
Kyo M, Harada H (1986) Control of the developmental pathway of tobacco pollen in vitro. Planta 168: 427–432
Kyo M, Yamaji N, Yuasa Y, Maeda T, Fukui H (2002) Isolation of cDNA coding for NtEPb1-b3, marker proteins for pollen dedifferentiation in a tobacco pollen culture system. Plant Sci 163: 1055–1062
Kyo M, Hattori S, Yamaji N, Pechan P, Fukui H (2003) Cloning and characterization of cDNAs associated with the embryogenic dedifferentiation of tobacco immature pollen grains. Plant Sci 163: 1055–1062
Maraschin SF Priester W de Spaink HP Wang M (2005) Androgenic switch: an example of plant embryogenesis from the male gametophyte perspective J Exp Bot 56: 1711–1726
Milla SR, Levin JS, Lewis RS, Rufty RC (2005) RAPD and SCAR markers linked to an intro- gressed Gene Conditioning Resistance to Peronospora tabacina D.B Adam in tobacco. Crop Sci 45: 2346–2354
Murad L, Yoong Lim K, Christopodulou V, Matyasek R, Lichtenstein CP, Kovarik A, Leitch AR (2002) The origin of tobacco's T genome is traced to a particular lineage within Nicotiana tomentosiformis (Solanaceae). Am J Bot 89: 921–928
Nakata K, Tanaka M (1968) Differentiation of embryoids from developing germ cells in anther culture of tobacco. Jpn J Genet 43: 65–71
Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19: 389–404
Nitsch JP (1972) Haploid plants from pollen. Z Pflanzenzüchtg 67: 3–18
Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163: 85–87
Nitsch C, Norreel B (1973) Effet dun choc thermique sur le pouvoir embryogene du pollen de Datura innoxia cultive dans lanthere ou isole de lanthere CR Acad Sci Ser D 276: 303–306
Pechan PM, Keller WA (1988) Identification of potentially embryogenic microspores in Brassica napus. Physiol Plant 74: 377–384
Raina SK, Irfan ST (1998) High frequency embryogenesis and plantlet regeneration from isolated microspores of indica rice. Plant Cell Rep 17: 957–962
Rashid A, Siddiqui AW, Reinert J (1981) Ultrastructure of embryogenic pollen of Nicotiana taba-cum var Badischer Burley. Protoplasma 107: 375–385
Ribarits A, Mamun AN, Li S, Resch T, Fiers M, Heberle-Bors E, Liu CM, Touraev A (2007) Combination of reversible male sterility and doubled haploid production by targeted inactivation of cytoplasmic glutamine synthetase in developing anthers and pollen. Plant Biotechnol J 5: 483–494
Sunderland N (1971) Anther culture: a progress report. Sci Prog Oxf 59: 527–549
Sunderland N (1978) Strategies on the improvement of yields in anther culture. In: Proceedings of Symposium on Plant Tissue Culture. Science Press, Peking, pp 65–86
Sunderland N, Dunwell JM (1977) Anther and pollen culture. In: Street HE (ed) Plant Tissue and Cell Culture. Oxford, Blackwell, pp 223–265
Sunderland N, Wicks FM (1969) Cultivation of haploid plants from tobacco pollen. Nature 224: 1227
Sunderland N, Wicks FM (1971) Embryoid formation in pollen grains of Nicotiana tabacum. J Exp Bot 22: 213–226
Tai GCC (2005) Haploids in the improvement of Solanaceous species. In: Palmer CE, Keller WA, Kasha KJ (eds) Haploids in Crop Improvement II. Springer, Berlin/Heidelberg, pp 173–190
Touraev A, Heberle-Bors E (2003) Anther and microspore culture in tobacco. In: Maluszynski M et al. (eds) Doubled Haploid Production in Crop Plants. Dordrecht, Netherlands: Kluwer Academic Publishers pp 223–228
Touraev A, Ilham A, Vicente O, Heberle-Bors E (1996a) Stress-induced microspore embryogen-esis in tobacco: an optimized system for molecular studies. Plant Cell Rep 15: 561–565
Touraev A, Pfosser M, Vicente O, Heberle-Bors E (1996b) Stress as the major signal controlling the developmental fate of tobacco microspores: towards a unified model of induction of micro-spore/pollen embryogenesis. Planta 200: 144–152
Touraev A, Vicente O, Heberle-Bors E (1997) Initiation of microspore embryogenesis by stress. Trends Plant Sci 2: 285–303
Touraev A, Pfosser M, Heberle-Bors E (2001) The microspore: a haploid multipurpose cell. Adv Bot Res 35: 53–109
Tulecke WR (1953) A tissue derived from the pollen of Ginkgo biloba. Science 117: 599–600
Tulecke WR (1959) The pollen cultures of C.D. LaRue: a tissue from the pollen of Taxus. Bull Torrey Bot Club 86: 283–289
Tulecke WR, Sehgal N (1963) Cell proliferation from the pollen of Torreya nucifera. Contrib Boyce Thompson Inst 22: 153–163
Wenzel G, Thomas E (1974) Observations on the growth in culture of anthers of Secale cereale. Z Pflanzenzüchtg 72: 89–94
Wernsman EA (1993) Varied roles for the haploid sporophyte in plant improvement. In: Stalker HT, Murphy JP (eds) Plant Breeding in the 1990s. CAB International, Wallingford, pp 461–484
Zaki MAM, Dickinson HG (1990) Structural changes during the first divisions of embryos resulting from anther and microspore culture in Brassica napus. Protoplasma 156: 149–162
Zarsky V, Garrido D, Rihova L, Tupy J, Vicente O, Heberle-Bors E (1992) Derepression of the cell cycle by starvation is involved in the induction of tobacco pollen embryogenesis. Sex Plant Reprod 5: 189–194
Zarsky V, Garrido D, Eller N, Tupy J, Vicente O, Schöffl F, Heberle-Bors E (1995) The expression of a small heat shock gene is activated during induction of tobacco pollen embryogenesis by starvation. Plant Cell Environ 18: 139–147
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Belogradova, K., Lewicka, I., Heberle-Bors, E., Touraev, A. (2009). An Overview on Tobacco Doubled Haploids. In: Touraev, A., Forster, B.P., Jain, S.M. (eds) Advances in Haploid Production in Higher Plants. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8854-4_5
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