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Current Trends in Monoclonal Antibody Development and Manufacturing pp 323–344Cite as

Antibody Radiolabeling

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Part of the book series: Biotechnology: Pharmaceutical Aspects ((PHARMASP,volume XI))

Abstract

The concept of using radiolabeled antibodies in vivo dates back to 1949 when Pressman (1949) demonstrated that radiolabeled antibodies could be used to localize specific tissue. Using 131I radiolabeled anti-carcinoembryonic antigen antibodies, Goldenberg et al. (1978) imaged human colonic carcinoma xenografts and in doing so, set in motion the field of radiolabeled antibodies for use in imaging and therapy.

Antibody based imaging entails either an antibody, bivalent fragment F(ab′)2, monovalent fragment Fab′ or antibody construct (minibody, diabody, tribody, etc.), with either a gamma or positron emitting radioisotope, having an appropriate energy, and an external camera system. A list of radioisotopes suitable for imaging is shown in Table 19-1.

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Authors and Affiliations

  1. Canadian Molecular Imaging Center of Excellence (CMICE), MDS Nordion, University of Ottawa Heart Institute, Ottawa, ON, Canada

    Corinne Bensimon

  2. Science and Technology Partnerships, MDS Nordion, Ottawa, ON, Canada

    Russell Redshaw

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  1. Corinne Bensimon
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  2. Russell Redshaw
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Editors and Affiliations

  1. Genentech Inc., DNA Way 1, San Francisco, 94080, U.S.A.

    Steven J. Shire

  2. Omeros Corporation, Fifth Ave Ste 2600 1420, Seattle, 98101, U.S.A.

    Wayne Gombotz

  3. Boehringer Ingelheim Pharma KG, Birkendorfer Str. 65, Biberach, 88397, Germany

    Karoline Bechtold-Peters

  4. Genentech, Inc., DNA Way 1, South San Francisco, 94080, U.S.A.

    James Andya

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Bensimon, C., Redshaw, R. (2010). Antibody Radiolabeling. In: Shire, S., Gombotz, W., Bechtold-Peters, K., Andya, J. (eds) Current Trends in Monoclonal Antibody Development and Manufacturing. Biotechnology: Pharmaceutical Aspects, vol XI. Springer, New York, NY. https://doi.org/10.1007/978-0-387-76643-0_19

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