5. Conclusions
Taurine (2-aminoethanesulfonic acid) is one of the major intracellular β-amino acids in mammals and is required for a number of biological processes including membrane stabilization, osmoregulation, modulation of calcium flux, antioxidation, neuromodulation, cell proliferation, and immune systems. As taurine is essential to the fetus and newborn for their development, the transfer of taurine into fetus is important during embryonic development. However, the protein expression pattern of taurine biosynthesis and transportation in the early embryonic development stage is still unknown. Thus, we have investigated the gene expression of TauT and CSD, which is one of the rate-limiting enzymes of taurine biosynthesis, using reverse transcriptase-polymerase chain reaction (RT-PCR) in mouse and chicken embryos to identify the expression phase during embryonic development. Murine embryos aged 4.5 days expressed both mRNAs of TauT and CSD. To overcome difficulties in the analyses of very immature embryos, the chicken embryo was employed instead of murine one. The chicken embryo aged 3 days produced TauT mRNA in the heart, brain and eye. In the analyses on chicken whole embryos, mRNAs of CSD and TauT began to appear at 12 and 48 h, respectively. These data show that TauT and CSD mRNAs are expressed in early stage of embryonic development and taurine synthetic enzyme is expressed earlier than that of the TauT, indicating important roles of taurine in the developing fetus.
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Kim, H.W., Yoon, S.H., Park, T., Kim, B.K., Park, K.K., Lee, D.H. (2006). Gene Expressions of Taurine Transporter and Taurine Biosynthetic Enzyme during Mouse and Chicken Embryonic Development. In: Oja, S.S., Saransaari, P. (eds) Taurine 6. Advances in Experimental Medicine and Biology, vol 583. Springer, Boston, MA . https://doi.org/10.1007/978-0-387-33504-9_7
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