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Multiple PLA2 Isoforms Regulate Taurine Release in NIH3T3 Mouse Fibroblasts

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Taurine 6

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 583))

2. Conclusion

Swelling-induced taurine efflux from NIH3T3 cells reflects a dependence on volume-sensitive arachidonic acid release. Both iPLA2 and sPLA2 appear to be involved in swelling-induced arachidonic acid release, likely reflecting their specific subcellular localization and dependence on lipid packing rather than volume-induced modulation of the actin-based cytoskeleton. Melittin-induced taurine efflux and cell shrinkage reflect PLA2 activity, mainly sPLA2. Both swelling- and melittin-induced taurine release is strictly dependent on 5-LO metabolites and takes place via a DIDS-sensitive leak pathway. These data are consistent with the notion that changes in PLA2 activity are an early event in cell volume sensing in mammalian cells.

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Authors and Affiliations

  1. Department of Biochemistry, Institute for Molecular Biology and Physiology, Copenhagen, Denmark

    Ian Henry Lambert

  2. Dept. of Biochemistry, Institute for Molecular Biology and Physiology, The August Krogh Building, 13, Universitetsparken, DK-2100, Copenhagen, Denmark

    Stine Falsig Pedersen

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  1. Ian Henry Lambert
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  2. Stine Falsig Pedersen
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Editors and Affiliations

  1. The Center for Laboratory Medicine, Tampere University Hospital, PO Box 2000, FI-33521, Tampere, Finland

    Simo S. Oja

  2. Brain Research Center Medical School, University of Tampere, FI-33014, Finland

    Pirjo Saransaari

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Lambert, I.H., Pedersen, S.F. (2006). Multiple PLA2 Isoforms Regulate Taurine Release in NIH3T3 Mouse Fibroblasts. In: Oja, S.S., Saransaari, P. (eds) Taurine 6. Advances in Experimental Medicine and Biology, vol 583. Springer, Boston, MA . https://doi.org/10.1007/978-0-387-33504-9_10

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