Abstract
Membranes less attractive to embryos were tested as a replacement for nylon screens to prevent adherence of somatic embryos, cell clusters and cells to different sites of the bioreactor, a feature considered undesirable in plant cell suspension cultures. The results showed that the loss of embryogenic cell-mass could be halved by using silicone or track membranes. For aeration purposes, these membranes are as satisfactory as nylon screens conventionally used in cell lift impellers.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Apel P, Didyk A, Zhitariuk NI, Larionova IE, Mamonova TI, Orelovich OL, Samoilova LI & Yanini IV (1995) Properties of track membranes of different structure characteristics. Scientific Instrument Engineering 5: 50–56
Hegarty PK, Smart NJ, Scragg AH, & Fowler MW (1986) The aeration of Catharantus roseus L.G. Don suspension cultures in airlift bioreactors: The inhibitory effect at high aeration rates on culture growth. J. Exp. Bot. 37: 1911–1920
Kamada H & Harada H (1979) Studies on the organogenesis in carrot tissue cultures. I. Effects of growth regulators on somatic embryogenesis and root formation. Z. Pflanzenphysiol. 91: 255–266
Luttman R, Florek P & Preil W (1994) Silicone-tubing aerated bioreactors for somatic embryo production. Plant Cell, Tissue and Organ Culture 39: 157–170
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497
Nomura K & Komamine A (1985) Identification and isolation of single cells, that produce somatic embryos at a high frequency in carrot suspension culture. Plant Physiol. 79: 988–991
Reinert J (1958) Morphogenese und ihre Kontrolle an Gewebekulturen aus Carotten. Naturwissenschaften 45: 244–245
Reinert J (1959) Ueber die Kontrolle der Morphogenese und die Induktion von Adventivembryonen an Gewebekulturen aus Karotten. Planta 53: 318–333
Scragg AH (1993) Commercial and technical perspectives. In: Hunter C (ed) In Vitro Cultivation of Plant Cells (pp 151–178). Butterworth-Heinemann, Oxford
Sorvari S, Toldi O, Ahanen K, Viinamäki T, Hakonen T & Tahvonen R (1997) Using polysaccharides and galactomannans as gelling agents in capsule formation of artificial seeds. J. Am. Soc. Hortic. Sci. 122: 878–883
Steward FC, Mapes MO & Smith J (1958) Growth and organized development of cultured cells. I. Growth and division of freely suspended cells. American Journal of Botany 45: 693–703
Sung Z (1976) Turbidimetric determination of plant cell culture growth and its application. Plant physiol. 57: 460–462
Sung Z & Okimoto R (1981) Embryonic proteins in somatic embryos of carrot. Proc. Natl. Acad. Sci. U.S.A. 78: 3683–3687
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer
About this chapter
Cite this chapter
Sorvari, S., Mäkeläinen, R., Ahanen, K., Toldi, O. (2005). Membranes to reduce adherence of somatic embryos to the cell lift impeller of a bioreactor. In: Hvoslef-Eide, A.K., Preil, W. (eds) Liquid Culture Systems for in vitro Plant Propagation. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3200-5_7
Download citation
DOI: https://doi.org/10.1007/1-4020-3200-5_7
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3199-1
Online ISBN: 978-1-4020-3200-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)