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Liquid Culture Systems for in vitro Plant Propagation pp 21–40Cite as

Application of bioreactor design principles to plant micropropagation

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Abstract

Principles of oxygen consumption, oxygen transport, suspension, and mixing are discussed in the context of propagating aggregates of plant tissue in liquid suspension bioreactors. Although micropropagated plants have a relatively low biological oxygen demand (BOD), the relatively large tissue size and localization of BOD in meristematic regions will typically result in oxygen mass transfer limitations in liquid culture. In contrast to the typical focus of bioreactor design on gas-liquid mass transfer, it is shown that media-solid mass transfer limitations limit oxygen available for aerobic plant tissue respiration. Approaches to improve oxygen availability through gas supplementation and bioreactor pressurization are discussed. The influence of media components on oxygen availability are also quantified for plant culture media. Experimental studies of polystyrene beads in suspension in a 30-litre air-lift and stirred bioreactors are used to illustrate design principles for circulation and mixing. Potential limitations to the use of liquid suspension culture due to plant physiological requirements are acknowledged.

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering, The Pennsylvania State University, USA

    Wayne R. Curtis

Authors
  1. Wayne R. Curtis
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Correspondence to Wayne R. Curtis .

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Editors and Affiliations

  1. Department of Plant and Environmental Science, Agricultural University of Norway, Ås, Norway

    Anne Kathrine Hvoslef-Eide

  2. Formerly Federal Centre for Breeding Research on Cultivated Plants, Institute for Ornamental Plant Breeding, Ahrensburg, Germany

    Walter Preil

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© 2005 Springer

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Curtis, W.R. (2005). Application of bioreactor design principles to plant micropropagation. In: Hvoslef-Eide, A.K., Preil, W. (eds) Liquid Culture Systems for in vitro Plant Propagation. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3200-5_2

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