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Mass propagation of tropical crops in temporary immersion systems

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Liquid Culture Systems for in vitro Plant Propagation

Abstract

Temporary immersion systems (TIS) have been described for in vitro multiplication of a wide range of tropical crops. Laboratory protocols are available for shoot multiplication, somatic embryo and microtuber production. Seven species are now commercially propagated by this culture technique (Ananas comosus, Coffea arabica, Cymbopogon citratus, Musa sp., Phalaenopsis, Saccharum sp., Solanum tuberosum) with different regeneration pathways and a variety of TIS designs. Beside the development of methods for producing somatic embryos in TIS, shoot multiplication protocols are the most applied from the commercial point of view. RITA® proved to be a suitable tool for research and laboratory scale, but for commercial application larger vessels are frequently used. Most important tropical species are commercially propagated in TIS using twin flasks ranging from 5–10 litres.

Production strategies for plant propagation in TIS, either by organogenesis or somatic embryogenesis are discussed and examples are given to illustrate the different possibilities for TIS integration in propagation of Musa sp. and Solanum tuberosum for shoot multiplication and microtuber production. For sugarcane (Saccharum sp.) somatic embryo production in bioreactors, embryo germination in TIS and field performance of regenerated plants are described.

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Authors and Affiliations

  1. Institute of Plant Biotechnology, Central University of Las Villas, Carretera a Camajuaní Km 5 1/2, Santa Clara, Villa Clara, Cuba

    Elio Jiménez González

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  1. Elio Jiménez González
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Editors and Affiliations

  1. Department of Plant and Environmental Science, Agricultural University of Norway, Ås, Norway

    Anne Kathrine Hvoslef-Eide

  2. Formerly Federal Centre for Breeding Research on Cultivated Plants, Institute for Ornamental Plant Breeding, Ahrensburg, Germany

    Walter Preil

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© 2005 Springer

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González, E.J. (2005). Mass propagation of tropical crops in temporary immersion systems. In: Hvoslef-Eide, A.K., Preil, W. (eds) Liquid Culture Systems for in vitro Plant Propagation. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3200-5_12

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