Volume 1030 2013

Antiviral Methods and Protocols

Editors:

ISBN: 978-1-62703-483-8 (Print) 978-1-62703-484-5 (Online)

Table of contents (33 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Human Immunodeficiency Virus Type 1 (HIV-1)

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-9

      A Fluorescence-Based High-Throughput Screening Assay to Identify HIV-1 Inhibitors

    3. No Access

      Book Protocol

      Pages 11-17

      A Homogeneous Time-Resolved Fluorescence Assay to Identify Inhibitors of HIV-1 Fusion

    4. No Access

      Book Protocol

      Pages 19-24

      Identification of HIV-1 Reverse Transcriptase Inhibitors Using a Scintillation Proximity Assay

    5. No Access

      Book Protocol

      Pages 25-36

      Biochemical Screening Assays to Identify HIV-1 Integrase Inhibitors

    6. No Access

      Book Protocol

      Pages 37-55

      HIV-1 Genotyping of the Protease-Reverse Transcriptase and Integrase Genes to Detect Mutations That Confer Antiretroviral Resistance

  3. Hepatitis Viruses

    1. Front Matter

      Pages 57-57

    2. No Access

      Book Protocol

      Pages 59-79

      In Vitro Kinetic Profiling of Hepatitis C Virus NS3 Protease Inhibitors by Progress Curve Analysis

    3. No Access

      Book Protocol

      Pages 81-92

      A Novel Hepatitis C Virus NS5B Polymerase Assay of De Novo Initiated RNA Synthesis Directed from a Heteropolymeric RNA Template

    4. No Access

      Book Protocol

      Pages 93-103

      Selecting and Characterizing Drug-Resistant Hepatitis C Virus Replicon

    5. No Access

      Book Protocol

      Pages 105-117

      A Cellular Replicon-Based Phenotyping Assay to Determine Susceptibility of Hepatitis C Virus Clinical Isolates to NS3/4A Protease Inhibitors

    6. No Access

      Book Protocol

      Pages 119-127

      Expression and Purification of Hepatitis C Virus Protease from Clinical Samples

    7. No Access

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      Pages 129-136

      Surface Plasmon Resonance as a Tool to Select Potent Drug Candidates for Hepatitis C Virus NS5B Polymerase

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      Pages 137-149

      Amplification and Sequencing of the Hepatitis C Virus NS3/4A Protease and the NS5B Polymerase Regions for Genotypic Resistance Detection of Clinical Isolates of Subtypes 1a and 1b

    9. No Access

      Book Protocol

      Pages 151-161

      A Southern Blot Assay for Detection of Hepatitis B Virus Covalently Closed Circular DNA from Cell Cultures

    10. No Access

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      Pages 163-181

      In Vitro Phenotyping of Recombinant Hepatitis B Virus Containing the Polymerase/Reverse Transcriptase Gene from Clinical Isolates

  4. Flaviviruses

    1. Front Matter

      Pages 183-183

    2. No Access

      Book Protocol

      Pages 185-194

      Cell-Based Antiviral Assays for Screening and Profiling Inhibitors Against Dengue Virus

    3. No Access

      Book Protocol

      Pages 195-203

      A Duplex Real-Time RT-PCR Assay for Profiling Inhibitors of Four Dengue Serotypes

    4. No Access

      Book Protocol

      Pages 205-219

      High-Throughput Screening Using Dengue Virus Reporter Genomes

    5. No Access

      Book Protocol

      Pages 221-236

      Fluorimetric and HPLC-Based Dengue Virus Protease Assays Using a FRET Substrate

    6. No Access

      Book Protocol

      Pages 237-247

      Expression and Purification of Dengue Virus NS5 Polymerase and Development of a High-Throughput Enzymatic Assay for Screening Inhibitors of Dengue Polymerase

    7. No Access

      Book Protocol

      Pages 249-268

      Detection and Quantification of Flavivirus NS5 Methyl-Transferase Activities

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