Therapeutic Proteins

Methods and Protocols

Editors:

ISBN: 978-1-61779-920-4 (Print) 978-1-61779-921-1 (Online)

Table of contents (29 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-26

    Therapeutic Proteins

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    Pages 27-41

    Synthetic Antibody Libraries

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    Pages 43-60

    The Construction of “Phylomer” Peptide Libraries as a Rich Source of Potent Inhibitors of Protein/Protein Interactions

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    Pages 61-72

    Ribosome Display and Screening for Protein Therapeutics

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    Pages 73-84

    Yeast Display of Engineered Antibody Domains

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    Pages 85-102

    Expression, Purification, and Characterization of Engineered Antibody CH2 and VH Domains

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    Pages 103-126

    Engineering of Affibody Molecules for Therapy and Diagnostics

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    Pages 127-144

    Protein Design for Diversity of Sequences and Conformations Using Dead-End Elimination

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    Pages 145-156

    Design and Generation of DVD-Ig™ Molecules for Dual-Specific Targeting

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    Pages 157-175

    Engineering and Expression of Bibody and Tribody Constructs in Mammalian Cells and in the Yeast Pichia pastoris

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    Pages 177-185

    Use of E. coli for the Production of a Single Protein

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    Pages 187-202

    Folding Engineering Strategies for Efficient Membrane Protein Production in E. coli

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    Pages 203-219

    Transient Expression Technologies: Past, Present, and Future

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    Pages 221-225

    Stable Transfection Pools for Large Quantity of Protein Production

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    Pages 227-238

    Mammalian Stable Expression of Biotherapeutics

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    Pages 239-264

    Transgenic Expression of Therapeutic Proteins in Arabidopsis thaliana Seed

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    Pages 265-275

    Methods for Chromatographic Removal of Endotoxin

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    Pages 277-292

    Effectiveness of Various Processing Steps for Viral Clearance of Therapeutic Proteins: Database Analyses of Commonly Used Steps

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    Pages 293-313

    High-Throughput Quantitative N-Glycan Analysis of Glycoproteins

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    Pages 315-323

    High-Throughput Multimodal Strong Anion Exchange Purification and N-Glycan Characterization of Endogenous Glycoprotein Expressed in Glycoengineered Pichia pastoris

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