Protein Chromatography

Methods and Protocols

Editors:

ISBN: 978-1-60761-912-3 (Print) 978-1-60761-913-0 (Online)

Table of contents (30 protocols)

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  1. Front Matter

    Pages i-x

  2. Overviews

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-23

      A Digest of Protein Purification

    3. No Access

      Book Protocol

      Pages 25-33

      Gel-Filtration Chromatography

    4. No Access

      Book Protocol

      Pages 35-59

      Immunoaffinity Chromatography

    5. No Access

      Book Protocol

      Pages 61-71

      Avoiding Proteolysis During Protein Chromatography

    6. No Access

      Book Protocol

      Pages 73-85

      Scale-Up of Protein Purification: Downstream Processing Issues

    7. No Access

      Book Protocol

      Pages 87-101

      Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources

    8. No Access

      Book Protocol

      Pages 103-136

      Engineering Protein Stability

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      Book Protocol

      Pages 137-150

      Microfluidics in Protein Chromatography

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      Book Protocol

      Pages 151-175

      Tagging Recombinant Proteins to Enhance Solubility and Aid Purification

  3. Protocols

    1. Front Matter

      Pages 177-177

    2. No Access

      Book Protocol

      Pages 179-202

      Storage and Lyophilisation of Pure Proteins

    3. No Access

      Book Protocol

      Pages 203-213

      Differential Precipitation and Solubilization of Proteins

    4. No Access

      Book Protocol

      Pages 215-228

      Ion-Exchange Chromatography: Basic Principles and Application to the Partial Purification of Soluble Mammalian Prolyl Oligopeptidase

    5. No Access

      Book Protocol

      Pages 229-258

      Protein Quantitation and Analysis of Purity

    6. No Access

      Book Protocol

      Pages 259-280

      Purification of Proteins Fused to Glutathione S-Transferase

    7. No Access

      Book Protocol

      Pages 281-293

      Purification of Proteins Fused to Maltose-Binding Protein

    8. No Access

      Book Protocol

      Pages 295-309

      Purification of Proteins from Baculovirus-Infected Insect Cells

    9. No Access

      Book Protocol

      Pages 311-335

      Purification of Poly-Histidine-Tagged Proteins

    10. No Access

      Book Protocol

      Pages 337-348

      Immobilized Metal Affinity Chromatography/Reversed-Phase Enrichment of Phosphopeptides and Analysis by CID/ETD Tandem Mass Spectrometry

    11. No Access

      Book Protocol

      Pages 349-367

      Tag Removal by Site-Specific Cleavage of Recombinant Fusion Proteins

    12. No Access

      Book Protocol

      Pages 369-382

      Purification of Antibodies Using Affinity Chromatography

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