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Simultaneous LC–MS–MS Determination of Zolmitriptan and Its Active Metabolite N-Desmethylzolmitriptan in Human Plasma

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Abstract

A specific and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry method was developed for the determination of zolmitriptan and N-desmethylzolmitriptan in human plasma. The analytes and the internal standard (IS) paroxetine were extracted by liquid–liquid extraction with a mixture of saturated ethyl acetate:dichloromethane (4:1) and were separated using an isocratic mobile phase on a XTerra RP18 column. The mobile phase used was acetonitrile: 5 mM ammonium acetate: formic acid (50:50:0.053, v/v/v). Zolmitriptan and N-desmethylzolmitriptan in a range of 0.25–20 ng mL−1 were easily quantified. The validated method can be applied to pharmacokinetic and bioequivalence studies.

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Authors and Affiliations

  1. Novagenix Bioanalytical R&D Centre, Ankara, Turkey

    B. Kılıç, S. Toptan & S. Özilhan

  2. Faculty of Pharmacy, Gazi University, Ankara, Turkey

    T. Özden

Authors
  1. B. Kılıç
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  2. T. Özden
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  3. S. Toptan
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  4. S. Özilhan
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Corresponding author

Correspondence to B. Kılıç.

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Application of Separation Techniques in Turkey

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Kılıç, B., Özden, T., Toptan, S. et al. Simultaneous LC–MS–MS Determination of Zolmitriptan and Its Active Metabolite N-Desmethylzolmitriptan in Human Plasma. Chroma 66 (Suppl 1), 129–133 (2007). https://doi.org/10.1365/s10337-007-0293-8

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  • DOI: https://doi.org/10.1365/s10337-007-0293-8

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