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Next Generation Ligand Binding Assays—Review of Emerging Real-Time Measurement Technologies

  • Review Article
  • Theme: Emerging Technologies in the BioAnalytical Space Applied to Large Molecule Determinations
  • Published:
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ABSTRACT

Over the last few years, numerous ligand binding assay technologies that utilize real-time measurement have been introduced; however, an assemblage and evaluation of these technologies has not previously been published. Herein, we describe six emerging real-time measurement technologies: Maverick™, MX96 SPR™, NanoDLSay™, AMMP®/ViBE®, SoPrano™, and two Lab-on-a-Chip (LoC) microfluidic devices. The development stage gate of these technologies ranges from pre-commercial to commercially available. Due to the novelty, the application and utility of some of the technologies regarding bioanalysis are likely to evolve but it is our hope that this review will provide insight into the direction the development of real-time measurement technologies is moving and the vision of those that are taking us there. Following the technology discussions, a comprehensive summary table is presented.

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Author information

Authors and Affiliations

  1. Pfizer Global Research and Development, Groton, Connecticut, USA

    Stephanie Fraser

  2. MPI Research, Mattawan, Michigan, USA

    Mark Cameron

  3. Frontage Laboratories, Inc., Exton, Pennsylvania, USA

    Edward O’Connor

  4. MedImmune, Clinical Pharmacology and DMPK, Mountain View, California, USA

    Martin Schwickart

  5. Molecular Biomarkers & Diagnostics, Merck & Co., Inc., Whitehouse Station, New Jersey, USA

    Michael Tanen

  6. Janssen Research & Development, LLC., Spring House, Pennsylvania, USA

    Mark Ware

Authors
  1. Stephanie Fraser
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  2. Mark Cameron
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  3. Edward O’Connor
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  4. Martin Schwickart
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  5. Michael Tanen
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  6. Mark Ware
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Corresponding author

Correspondence to Stephanie Fraser.

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Guest Editors: Johanna Mora and Binodh Desilva

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Fraser, S., Cameron, M., O’Connor, E. et al. Next Generation Ligand Binding Assays—Review of Emerging Real-Time Measurement Technologies. AAPS J 16, 914–924 (2014). https://doi.org/10.1208/s12248-014-9643-2

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  • DOI: https://doi.org/10.1208/s12248-014-9643-2

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