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Effect of replacing organic grass-clover silage from primary growth with regrowth on feed intake and milk yield of dairy cows

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Abstract

Under Norwegian conditions, diets based on primary growth (PG) silage typically increase milk yield compared to silage prepared from the regrowth (RG). Organic PG, dominated by immature grasses, is often high in energy and low in crude protein (CP), whereas the opposite is the case for organic RG harvests, dominated by clover. Here, we tested the hypotheses that increasing proportions of RG will reduce the total supply of metabolizable energy, but increase the CP intake, and that there is a dietary optimal mix of PG and RG to meet requirements for optimal milk production. Sixteen Norwegian Red cows were used in an experiment designed with four balanced 4 × 4 Latin squares with 21-day periods to evaluate the effect of incremental replacement of PG with RG on feed intake, nutrient digestion, and milk production. Silages were prepared from PG and RG of an organically managed grassland. Treatments comprised silages fed ad libitum with RG replacing PG in ratios of 0, 0.33, 0.67, and 1 on dry matter (DM) basis. Additionally, concentrate was offered with 8 kg for pluriparous and 7 kg for primiparous cows. The PG had higher content metabolizable energy (ME), potentially degradable neutral detergent fiber (NDF), and water-soluble carbohydrates, while RG contained more CP and indigestible NDF. The already mentioned characteristics led to higher intakes of DM, organic matter, NDF, and ME and lower intakes of CP and indigestible NDF with increasing proportions of PG in the diet. Milk yield tended to be higher when PG and RG were offered as a mixture than when fed alone. The milk fat concentration decreased linearly with increasing proportions of RG proportion, while protein concentration was unaffected by diet. This led to a similar production of energy-corrected milk among cows fed diets containing PG while cows fed pure RG diet produced 0.9 kg less daily. Silage energy concentration and energy intake influenced milk production more than CP supply.

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References

  • AOAC (1984) AOAC official method 7.074 fiber (acid detergent) and lignin in animal feed. In: Williams S (ed) Official methods of analysis of AOAC, 14th edn. Assoc. of Off. Anal. Chemists, Arlington, pp 162–163

    Google Scholar 

  • Beauchemin K (1991) Effects of dietary neutral detergent fiber concentration and alfalfa hay quality on chewing, rumen function, and milk production of dairy cows. J Dairy Sci 74:3140–3151

    Article  CAS  PubMed  Google Scholar 

  • Bertilsson J, Murphy M (2003) Effects of feeding clover silages on feed intake, milk production and digestion in dairy cows. Grass Forage Sci 58:309–322. doi:10.1046/j.1365-2494.2003.00383.x

    Article  Google Scholar 

  • Byström S, Jonsson S, Martinsson K (2002) Organic versus conventional dairy farming—studies from the Öjebyn project. The UK Organic Research Conference, Aberystwyth, pp 179–184

    Google Scholar 

  • Counc. of the Eur. Union (2007) Council Regulation (EC) No. 834/2007 of 28 June 2007 on organic production and labelling of organic products and repealing Regulation (EEC) No 2092/91. Off J Eur Communities 189:1–23

    Google Scholar 

  • Dewhurst R, Fisher W, Tweed J, Wilkins R (2003) Comparison of grass and legume silages for milk production. 1. Production responses with different levels of concentrate. J Dairy Sci 86:2598–2611

    Article  CAS  PubMed  Google Scholar 

  • Eriksson T, Norell L, Nilsdotter-Linde N (2012) Nitrogen metabolism and milk production in dairy cows fed semi-restricted amounts of ryegrass–legume silage with birdsfoot trefoil (Lotus corniculatus L.) or white clover (Trifolium repens L.). Grass Forage Sci 67:546–558. doi:10.1111/j.1365-2494.2012.00882.x

    Article  CAS  Google Scholar 

  • Govasmark E, Steen A, Bakken A, Steinshamn H, Strøm T, Hansen S (2005) Factors affecting the concentration of Zn, Fe and Mn in herbage from organic farms and in relation to dietary requirements of ruminants. Acta Agri Scand, B—Soil Plant Sci 55:131–142. doi:10.1080/09064710510008586

    CAS  Google Scholar 

  • Halmemies-Beauchet-Filleau A, Vanhatalo A, Toivonen V, Heikkilä T, Lee MRF, Shingfield KJ (2014) Effect of replacing grass silage with red clover silage on nutrient digestion, nitrogen metabolism, and milk fat composition in lactating cows fed diets containing a 60:40 forage-to-concentrate ratio. J Dairy Sci 97:3761–3776. doi:10.3168/jds.2013-7358

    Article  CAS  PubMed  Google Scholar 

  • Huhtanen P, Hristov AN (2009) A meta-analysis of the effects of dietary protein concentration and degradability on milk protein yield and milk N efficiency in dairy cows. J Dairy Sci 92:3222–3232. doi:10.3168/jds.2008-1352

    Article  CAS  PubMed  Google Scholar 

  • Huhtanen P, Nousiainen JI, Khalili H, Jaakkola S, Heikkilä T (2003) Relationships between silage fermentation characteristics and milk production parameters: analyses of literature data. Livest Prod Sci 81:57–73

    Article  Google Scholar 

  • Huhtanen P, Nousiainen J, Rinne M et al (2006) Recent developments in forage evaluation with special reference to practical applications. Agri Food Sci 15:31

    Google Scholar 

  • Huhtanen P, Rinne M, Nousiainen J (2007) Evaluation of the factors affecting silage intake of dairy cows: a revision of the relative silage dry-matter intake index. Animal 1:13

    Article  Google Scholar 

  • SAS Institute Inc (2011) SAS/STAT Software, Version 9.3. doi:citeulike-article-id:11105873

  • Khalili H, Huhtanen P (1991) Sucrose supplements in cattle given grass silage-based diet. 2. Digestion of cell wall carbohydrates. Anim Feed Sci Tech 33:263–273. doi:10.1016/0377-8401(91)90065-Z

    Article  Google Scholar 

  • Khalili H, Sairanen A, Nousiainen J, Huhtanen P (2005) Effects of silage made from primary or regrowth grass and protein supplementation on dairy cow performance. Livest Prod Sci 96:269–278

    Article  Google Scholar 

  • Kuoppala K, Rinne M, Nousiainen J, Huhtanen P (2008) The effect of cutting time of grass silage in primary growth and regrowth and the interactions between silage quality and concentrate level on milk production of dairy cows. Livest Sci 116:171–182. doi:10.1016/j.livsci.2007.10.002

    Article  Google Scholar 

  • Kuoppala K, Ahvenjarvi S, Rinne M, Vanhatalo A (2009) Effects of feeding grass or red clover silage cut at two maturity stages in dairy cows. 2. Dry matter intake and cell wall digestion kinetics. J Dairy Sci 92:5634–5644. doi:10.3168/jds.2009-2250

    Article  CAS  PubMed  Google Scholar 

  • Kuoppala K, Rinne M, Ahvenjarvi S, Nousiainen J, Huhtanen P (2010) The effect of harvesting strategy of grass silage on digestion and nutrient supply in dairy cows. J Dairy Sci 93:3253–3263. doi:10.3168/jds.2009-3013

    Article  CAS  PubMed  Google Scholar 

  • Leiber F (2014) Resigning protein concentrates in dairy cattle nutrition: a problem or a chance? Org Agri 4:269–273

    Article  Google Scholar 

  • Licitra G, Hernandez TM, VanSoest PJ (1996) Standardization of procedures for nitrogen fractionation of ruminant feeds. Anim Feed Sci Tech 57:347–358. doi:10.1016/0377-8401(95)00837-3

    Article  Google Scholar 

  • Madsen J, Hvelplund T, Weisbjerg MR et al. (1995) The AAT/PBV protein evaluation system for ruminants—a revision. Nor J Agri Sci Suppl 35

  • National Research Council (2001) Nutrient requirements of dairy cattle: seventh revised edition, 2001. The Natl. Acad Press, Washington, DC

    Google Scholar 

  • Newton G, Fontenot J, Tucker R, Polan C (1972) Effects of high dietary potassium intake on the metabolism of magnesium by sheep. J Anim Sci 35:440–445

    Article  CAS  PubMed  Google Scholar 

  • NorFor (2007a) Norfor in sacco standard, Nordic feed evaluation system. Accessed 21.11 2014

  • Norfor (2007b) Norfor method for determination of dry matter vol 2015, 21 September 2007 edn

  • Nousiainen J (2004) Development of tools for the nutritional management of dairy cows on silage-based diets. PhD Thesis. Univ. of Helsinki

  • Peoples A, Gordon F (1989) The influence of wilting and season of silage harvest and the fat and protein concentration of the supplement on milk production and food utilization by lactating cattle. Anim Prod 48:305–317

    Article  Google Scholar 

  • TINE Rådgiving (2012) TINE SA (The Nor. Dairy Ass.), Ås, Norway

  • Randby ÅT (1992) Grass-clover silage for dairy cows. In: 14th Gen. Meet. of Eur. Grassl. Fed., Lahti, Finland, 8–11 June 1992 1992. pp 272–275

  • Randby Å, Nørgaard P, Weisbjerg M (2010) Effect of increasing plant maturity in timothy‐dominated grass silage on the performance of growing/finishing. Norwegian Red Bulls Grass Forage Sci 65:273–286

    CAS  Google Scholar 

  • Randby Å, Weisbjerg M, Nørgaard P, Heringstad B (2012) Early lactation feed intake and milk yield responses of dairy cows offered grass silages harvested at early maturity stages. J Dairy Sci 95:14

    Article  Google Scholar 

  • Schwab C, Huhtanen P, Hunt C, Hvelplund T (2005) Nitrogen requirements of cattle. In: Pfeffer E, Hristov AN (eds) Nitrogen and phosphorus nutrition of cattle: reducing the environmental impact of cattle operations, vol 1. CABI Publ, Cambridge, pp 13–70

    Chapter  Google Scholar 

  • Spörndly R (2003) Fodertabeller för idisslare. Rep. 257. Dep. of Anim. Nutr. Manag. Swedish Univ. Agric. Sci, Uppsala

    Google Scholar 

  • Steinshamn H (2001) Effects of cattle slurry on the growth potential and clover proportion of organically managed grass-clover leys. Acta Agri Scand, B - Soil Plant Sci 51:12. doi:10.1080/09064710127615

    Google Scholar 

  • Steinshamn H (2010) Effect of forage legumes on feed intake, milk production and milk quality—a review Anim Sci Pap and Rep 28:12

  • Steinshamn H, Thuen E (2008) White or red clover-grass silage in organic dairy milk production: grassland productivity and milk production responses with different levels of concentrate. Livest Sci 119:14. doi:10.1016/j.livsci.2008.04.004

    Article  Google Scholar 

  • Stensig T, Weisbjerg MR, Hvelplund T (1998) Digestion and passage kinetics of fibre in dairy cows as affected by the proportion of wheat starch or sucrose in the diet. Acta Agri Scand, A - Anim Sci 48:12. doi:10.1080/09064709809362413

    Google Scholar 

  • Van Es AJH (1978) Feed evaluation for ruminants. I. The systems in use from May 1977-onwards in the Netherlands. Livest Prod Sci 5:15. doi:10.1016/0301-6226(78)90029-5

    Google Scholar 

  • Van Soest PJ (1994) Nutritional ecology of the ruminant, 2nd edn. Comstock Publ. Assoc, Ithaca

    Google Scholar 

  • Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74:15. doi:10.3168/jds.S0022-0302(91)78551-2

    Google Scholar 

  • Vanhatalo A (2008) Effects of harvest time of red clover silage on milk production and composition. In: biodivers. and anim. feed: future chall. for grassl. prod. Proceedings of the 22nd Gen. Meet. of the Eur. Grassl. Fed. Swedish Univ. of Agri. Sci, Uppsala, pp 391–393

    Google Scholar 

  • Vanhatalo A, Kuoppala K, Ahvenjärvi S, Rinne M (2009) Effects of feeding grass or red clover silage cut at two maturity stages in dairy cows. 1. Nitrogen metabolism and supply of amino acids. J Dairy Sci 92:14. doi:10.3168/jds.2009-2249

    Article  Google Scholar 

  • Volden H (2011) NorFor Nordic feed evaluation system Norfor—the Nordic feed evaluation system, vol 130. Wageningen Acad. Publ, Wageningen. doi:10.3920/978-90-8686-718-9

    Book  Google Scholar 

Download references

Acknowledgments

The project was funded by the Norwegian Agricultural Agreement Research Fund (Project number 207755 in The Research Council of Norway), the County Governors of Sør- and Nord-Trøndelag, the Sør- and Nord-Trøndelag County Authorities, TINE SA, and the Norwegian Agricultural Extension Service. The authors have no financial or other conflict of interest in the manuscript. Further, the authors acknowledge Torstein Garmo for his help with botanical composition, Egil Prestløkken for conducting a digestibility experiment with our feeds, and, finally, the always present and helpful staff at the experimental unit led by Dag Kristoffer Forberg.

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Correspondence to Sondre Stokke Naadland.

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Naadland, S.S., Steinshamn, H. & Randby, Å.T. Effect of replacing organic grass-clover silage from primary growth with regrowth on feed intake and milk yield of dairy cows. Org. Agr. 7, 41–51 (2017). https://doi.org/10.1007/s13165-015-0144-0

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