Abstract
Blast disease caused by the fungus, Magnaporthe grisea, is among the most damaging diseases of rice and ragi leading to heavy crop losses worldwide. To avoid losses, farmers resort to indiscriminate applications of plant protection chemicals that also harm the ecosystem and lead to resistance buildup in the phytopathogens. Therefore, alternative eco-friendly strategies need to be evolved and put in place. The present study was thus undertaken to identify competent rhizobacteria having promising biocontrol activity against M. grisea infecting ragi. A total of 70 rhizospheric pseudomonads were isolated from different annual plants, of which 10 isolates showed maximum inhibition of two test isolates of M. grisea. Pseudomonas fluorescens isolate Pf-30 exhibited maximum inhibition (81.25 and 88.43 %) against the test pathogen, followed by Pf-53. Of all, 19 isolates were prominent in chitinase production with Pf-30 showing maximum efficiency (218.18 %) for enzyme production. In general, 25 °C temperature and pH 5.0 were optimum for enzymatic activity, although maximum activity (15.42 IU ml−1) by Pf-30 was recorded at pH 6.5 and 35 °C, followed by Pf-53 (4.48 IU ml−1). In-planta evaluation in polyhouse revealed that different pseudomonads could suppress the disease significantly when given as seed treatment and foliar spray. Maximum disease suppression was exhibited by Pf-47 and Pf-53 (82.77 and 82.06 %, respectively). Effect of three-factor (pathogen, variety, and pseudomonads) interaction revealed that all interactions, except pathogen x variety, contributed significantly.
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References
Ayyadurai N, Ravindra Naik P, Sakthivel N (2007) Functional characterization of antagonistic fluorescent pseudomonads associated with rhizospheric soil of rice (Oryza sativa L.). J Microbiol Biotechnol 17:919–927
Bhanti M, Taneja A (2007) Contamination of vegetables of different seasons with organophosphorous pesticides and related health risk assessment in northern India. Chemosphere 69:63–68
Chang WT, Shen CS, Wang SL (2003) An antifungal chitinase produced by Bacillus cereus with shrimp and crab shell powder as a carbon source. Curr Microbiol 47:102–108
Chernin L, Chet I (2002) Microbial enzymes in biocontrol of plant pathogens and pests. In: Burns R, Dick R (eds) Enzymes in the environment: activity, ecology, and applications. Marcel Dekker, Inc., New York, pp 171–225
Frandberg E, Schnurer J (1998) Antifungal activity of chitinolytic bacteria isolated from airtight stored cereal grain. Can J Microbiol 44:121–127
Gohel V, Singh A, Vimal M, Ashwini P, Chhatpar HS (2006) Bioprospecting and antifungal potential of chitinolytic microorganisms. Afr J Biotechnol 5:54–72
Green AT, Heal MG, Healy A (2005) Production of chitinolytic enzymes by Serratia marcescens QM B 1496 using various chitinous substrates. J Chem Technol 80:28–34
Gurusubramanian G, Rahman A, Sarmah M, Ray S, Bora S (2008) Pesticide usage pattern in tea ecosystem, their retrospects and alternative measures. J Environ Biol 29:813–826
Hu X, Roberts DP, Xie L, Maul JE, Yu C, Li Y, Jiang M, Liao X, Che Z, Liao X (2014) Formulations of Bacillus subtilis BY-2 suppress Sclerotinia sclerotiorum on oilseed rape in the field. Biol Contr 70:54–64
Karthikeyan V, Gnanamanickam SS (2008) Biological control of Setaria blast (Magnaporthe grisea) with bacterial strains. Crop Prot 27:263–267
Kohli U, Dogra RC, Kapoor KK, Sharma PK (2006) Chitinolytic Pseudomonas maltophilia antagonistic to root rot pathogens of sunflower (Helianthus annuus). Ind J Microbiol 46:45–49
Krishnamurthy J, Samiyappan R, Vidhyasekaran P, Nakkeeran S, Rajeswari E, Raja JAJ, Balasubramanian P (1999) Efficacy of Trichoderma chitinases against Rhizoctonia solani, the rice sheath blight pathogen. J Bioscciences 24:207–213
Kumar B, Kumar J (2011) Management of blast disease of finger millet (Elusine coracana) through fungicides, bioagents and varietal mixture. Ind Phytopathol 64:272–274
Kumar J, Nelson RJ, Zeigler RS (1999) Population structure and dynamics of Magnaporthe grisea in the Indian Himalayas. Genetics 152:971–984
Leung H, Borromeo ES, Bernardo MA, Notteghem JL (1988) Genetic analysis of virulence in the rice blast fungus Magnaporthe grisea. Phytopathol 78:1227–1233
Lin Y, Du D, Si C, Zhao Q, Li Z, Li P (2014) Potential biocontrol Bacillus sp. strains isolated by an improved method from vinegar waste compost exhibit antibiosis against fungal pathogens and promote growth of cucumbers. Biol Contr 71:7–15
Mejia-Saules JM, Waliszewski KN, Garcia MA, Cruz-Camarilla R (2006) The use of crude shrimp shell powder for chitinase production by Serratia marcescens WF. Food Technol Biotecnol 44:646–651
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
Monreal J, Reese ET (1969) The chitinase of Serratia marcescens. Can Microbiol 15:689–696
Negi YK, Garg SK, Kumar J (2005) Cold tolerant fluorescent Pseudomonas isolated from Garhwal Himalayas as potential plant growth promoting and biocontrol agents in pea. Curr Sci 89:2151–2156
Negi YK, Garg SK, Kumar J (2008) Plant growth promoting and biocontrol activities of cold-tolerant Pseudomonas fluorescens isolates against root rot in pea. Ind Phytopathol 61:461–470
Negi YK, Prabha D, Garg SK, Kumar J (2011) Genetic diversity among cold-tolerant fluorescent Pseudomonas isolates from Indian Himalayas and their characterization for biocontrol and plant growth promoting activities. J Pl Growth Regul 30:128–143
Pankaj K, Maheshwari DK, Dubey RC (2012) Bacillus strains isolated from rhizosphere showed plant growth promoting and antagonistic activity against phytopathogens. Microbiol Res 167:493–499
Patro TSSK, Rani C, Kumar GV (2008) Pseudomonas fluorescens, a potential bioagent for the management of blast disease in Elusine coracana. J Mycol Pl Pathol 38:298–300
Pore MS, Magar NG (1979) Nutrient composition of hybrid varieties of finger millet. Ind J Agric Sci 49:526–531
Radjacommare R, Ramanathan A, Kandan A, Sible GV, Harish S, Samiyappan R (2004) Purification and antifungal activity of chitinase against Pyricularia grisea in finger millet. World J Microbiol Biotechnol 20:251–256
Ridout CJ, Coley-Smith JR, Linch JM (1986) Enzyme activity and electrophoretic profile of extracellular proteins induced in Trichoderma spp. by cell walls of Rhizoctonia solani. J Gen Microbiol 132:2345–2352
Robert WK, Selitrennikoff CP (1988) Plant and bacterial chitinases differ in antifungal activity. J Gen Microbiol 134:169–176
Singh PB, Singh V, Nay PK (2008) Pesticide residues and reproductive dysfunction in different vertebrates from north India. Food Chem Toxicol 46:2533–2539
Skidmore AM, Dickinson CH (1976) Colony interaction and hyphal interference between Septoria nodorum and phylloplane fungi. Trans Brit Mycol Soc 66:57–74
Talbot NJ, Ebbole DJ, Hamer JE (1993) Identification and characterization of Mpg1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Plant Cell 5:1575–1590
Vishwanathan R, Samiyappan R (1999) Induction of systemic resistance by plant growth promoting rhizobacteria against red rot diseases caused by Colletotrichum falcatum went in sugarcane. In: Proc Sugar Technology Association India 61:24–39
Xiao-Jing X, Li-Qun Z, You-Yong Z, Wen-Hua T (2005) Improving biocontrol effect of Pseudomonas fluorescens P5 on plant diseases by genetic modification with chitinase gene. Chin J Agric Biotechol 2:23–27
Zeigler RS, Leong SA, Teng PS (1994) Rice blast disease. CAB International, Wallingford, p 626
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Negi, Y.K., Prabha, D., Garg, S.K. et al. Biological control of ragi blast disease by chitinase producing fluorescent Pseudomonas isolates. Org. Agr. 7, 63–71 (2017). https://doi.org/10.1007/s13165-015-0142-2
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DOI: https://doi.org/10.1007/s13165-015-0142-2