Molecular sexing of tigers, Panthera tigris
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- McEwing, R., Ouitavon, K., Rovie-Ryan, J.J. et al. Conservation Genet Resour (2012) 4: 299. doi:10.1007/s12686-011-9529-x
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We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. The ZFX and ZFY amplicons differ in size by 12 bp and can thus be differentiated by capillary electrophoresis.