Technical Note

Conservation Genetics Resources

, Volume 4, Issue 2, pp 299-301

Molecular sexing of tigers, Panthera tigris

  • R. McEwingAffiliated withRoyal Zoological Society of Scotland Email author 
  • , K. OuitavonAffiliated withDNP Wildlife Forensic Science Unit, Wildlife Conservation Office, Department of National Parks, Wildlife and Plant Conservation
  • , J. J. Rovie-RyanAffiliated withWildlife Genetic Resource Bank (WGRB) Laboratory, Department of Wildlife and National Parks
  • , WulansariAffiliated withEijkman Institute for Molecular Biology
  • , F. T. SitamAffiliated withWildlife Genetic Resource Bank (WGRB) Laboratory, Department of Wildlife and National Parks
  • , R. OgdenAffiliated withRoyal Zoological Society of Scotland

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. The ZFX and ZFY amplicons differ in size by 12 bp and can thus be differentiated by capillary electrophoresis.

Keywords

Molecular sex identification Tiger Zinc finger protein Forensics Felids