Skip to main content

Advertisement

SpringerLink
Log in

miR-150 regulates B lymphocyte in autoimmune hemolytic anemia/Evans syndrome by c-Myb

  • Original Article
  • Published:
International Journal of Hematology Aims and scope Submit manuscript

Abstract

The objective of the study was to study the regulation of B lymphocytes in patients with autoimmune hemolytic anemia (AIHA)/Evans syndrome. From October 2015 to May 2016, 35 patients with AIHA/Evans in the Department of Hematology, Tianjin Medical University General Hospital were enrolled into this study. c-Myb mRNA and miR-150 expression in B lymphocytes were measured using real-time PCR (RT-PCR). Correlation between c-Myb and miR-150 and clinical parameters in patients with AIHA/Evans were analyzed. c-Myb mRNA expression in hemolysis patients was significantly higher than in remission patients and CLL patients, negatively correlated with hemoglobin (Hb) level and complement 3(C3) levels, and positively correlated with total bilirubin (TBIL) concentration and indirect bilirubin (IBIL) concentration. miR-150 expression in hemolysis patients was significantly lower than in patients in remission and CLL patients. miR-150 was negatively correlated with TBIL and IBIL, and positively correlated with Hb, C3. c-Myb mRNA expression levels in hemolytic episode patients were negatively correlated with the expression levels of miR-150. The expression of c-Myb, a regulatory factor of B lymphocytes, is increased in B lymphocytes of AIHA/Evans patients, while miR-150 expression is decreased. c-Myb was negatively correlated with miR-150.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. He Y, Jiang X, Chen J. The role of miR-150 in normal and malignant hematopoiesis. Oncogene. 2014;33(30):3887–93.

    Article  PubMed  CAS  Google Scholar 

  2. Garo LP, Murugaiyan G. Contribution of MicroRNAs to autoimmune diseases. Cell Mol Life Sci. 2016;73(10):2041–51.

    Article  PubMed  CAS  Google Scholar 

  3. Lin YC, Kuo MW, Yu J, et al. c-Myb is an evolutionary conserved miR-150 target and miR-150/c-Myb interaction is important for embryonic development. Mol Biol Evol. 2008;25(10):2189–98.

    Article  PubMed  CAS  Google Scholar 

  4. Xiao C, Calado DP, Galler G, et al. MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell. 2007;131(1):146–59.

    Article  PubMed  CAS  Google Scholar 

  5. Zhinan Z. Criteria of diagnosis and curative effect of hematopathy. 3rd ed. China: Science Press; 2007. p. 68–71.

    Google Scholar 

  6. Neff AT. Autoimmune hemolytic anemias. In: Greer JP, Foerster J, Lukens JN, Rodgers GM, Paraskevas F, Glader B, editors. Wintrobe’s clinical hematology. Philadelphia: Lippincott Williams & Wilkins; 2004. p. 1157–82.

    Google Scholar 

  7. Li K, Tie H, Hu N, et al. Association of two polymorphisms rs2910164 in miRNA-146a and rs3746444 in miRNA-499 with rheumatoid arthritis: a meta-analysis. Hum Immunol. 2014;75(7):602–8.

    Article  PubMed  CAS  Google Scholar 

  8. Lai NS, Hui-Chun Y, Tung CH, Huang KY, Huang HB, Ming-Chi L. The role of aberrant expression of T cell miRNAs affected by TNF-α in the immunopathogenesis of rheumatoid arthritis. Arthr Res Ther. 2017;19(1):261.

    Article  Google Scholar 

  9. Fu L, Jin YL, et al. Comprehensive review of genetic association studies and meta-analysis on miRNA polymorphisms and rheumatoid arthritis and systemic lupus erythematosus susceptibility. Hum Immunol. 2016;77(1):1–6.

    Article  PubMed  CAS  Google Scholar 

  10. Nogales-Gadea G, Ramos-Fransi A, Suárez-Calvet X, et al. Analysis of serum miRNA profiles of myasthenia gravis patients. PLoS One. 2014;9(3):e91927.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  11. Burenbatu, Borjigin M, Eerdunduleng, et al. Profiling of miRNA expression in immune thrombocytopenia patients before and after Qishunbaolier (QSBLE) treatment. Biomed Pharmacother. 2015;75:196–204.

    Article  PubMed  CAS  Google Scholar 

  12. Ferrer G, Navarro A, Hodgson K, et al. MicroRNA expression in chronic lymphocytic leukemia developing autoimmune hemolytic anemia. Leuk Lymphoma. 2013;54(9):2016–22.

    Article  PubMed  CAS  Google Scholar 

  13. Stenman G, Andersson MK, Andren Y. New tricks from an old oncogene. Cell Cycle. 2010;9(15):2986–95.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  14. Stamatopoulos B, Van Damme M, Crompot E, et al. Opposite prognostic significance of cellular and serum circulating MicroRNA-150 in patients with chronic lymphocytic leukemia. Mol Med. 2015;21:123–33.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  15. Thomas MD, Kremer CS, Ravichandran KS. c-Myb is critical for B cell development and maintenance of follicular B cells. Immunity. 2005;23:275–86.

    Article  PubMed  CAS  Google Scholar 

  16. Greig KT, de Graaf CA, Murphy JM, et al. Critical roles for c-Myb in lymphoid priming and early B-cell development. Blood. 2010;115:2796–805.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  17. Wei H, Ding XC, Ren JG, et al. A murine model for human immune thrombocytopenic purpura and comparative analysis of multiple gene expression in bone marrow and spleen. J Genet Genomics. 2008;35(11):665–71.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by grants from Application Foundation and Advanced Technology Research Project of Tianjin (15JCYBJC27300), National Natural Science Foundation of China (81370607), Science and Technology Foundation of Tianjin Health Bureau (09KZ108).

Author information

Author notes

  1. Limin Xing and Wenyan Xu are co-first authors.

Authors and Affiliations

  1. Hematology Department of General Hospital, Tianjin Medical University, Tianjin, 300052, China

    Limin Xing, Wenyan Xu, Yingying Qu, Manjun Zhao, Hong Liu, Huaquan Wang & Zonghong Shao

  2. Hematology department of the 1st People’s Hospital of Jining, Shandong, 272011, China

    Hongli Zhu

  3. Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200092, China

    Xin Su

Authors
  1. Limin Xing
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wenyan Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yingying Qu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Manjun Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hongli Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hong Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Huaquan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xin Su
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Zonghong Shao
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

XLM and SX participated in manuscript writing. SZH and XLM designed this study. LH and WHQ collected the cases. XWY, ZHL, QYY and ZMJ took part in the study.

Corresponding authors

Correspondence to Limin Xing or Zonghong Shao.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xing, L., Xu, W., Qu, Y. et al. miR-150 regulates B lymphocyte in autoimmune hemolytic anemia/Evans syndrome by c-Myb. Int J Hematol 107, 666–672 (2018). https://doi.org/10.1007/s12185-018-2429-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12185-018-2429-z

Keywords

Search

Navigation