Molecular Biotechnology

, Volume 52, Issue 2, pp 101–110

ENAC1, a NAC Transcription Factor, is an Early and Transient Response Regulator Induced by Abiotic Stress in Rice (Oryza sativa L.)

Research

DOI: 10.1007/s12033-011-9477-4

Cite this article as:
Sun, H., Huang, X., Xu, X. et al. Mol Biotechnol (2012) 52: 101. doi:10.1007/s12033-011-9477-4
  • 800 Downloads

Abstract

The plant-specific NAC (NAM, ATAF, and CUC)-domain proteins play important roles in plant development and stress responses. In this research, a full-length cDNA named ENAC1 (early NAC-domain protein induced by abiotic stress 1) was isolated from rice. ENAC1 possess one NAC domain in the N-terminus. Comparative time-course expression analysis indicated that ENAC1 expression, similar with OsDREB1A, was induced very quickly by various abiotic stresses including salt, drought, cold, and exogenous abscisic acid. However, the induction of ENAC1 by abiotic stress was transient and lasted up to 3 h, whereas that of OsDREB1A maintained longer. The promoter sequence of ENAC1 harbors several cis-elements including ABA response elements, but the well-known dehydration responsive element/C-repeat element is absent. The ENAC1-GFP (green fluorescent protein) fusion protein was localized in the nucleus of rice protoplast cell. Yeast hybrid assays revealed that ENAC1 was a transcription activator and bound to NAC recognition sequence (NACRS). Co-expression analysis suggested that ENAC1 co-expressed with a number of stress-related genes. Taken together, ENAC1 may be an early transcription activator of stress responses and function in the regulation of NACRS-mediated gene expression under abiotic stress.

Keywords

RiceTranscription factorNACAbiotic stressABA

Abbreviations

ABA

Abscisic acid

ABRE

ABA response elements

CRT

C-repeat

DAPI

4,6-Diamidino-2-phenylindole

DRE

Dehydration responsive element

DREB

Dehydration responsive element binding protein

GFP

Green fluorescent protein

NAC

NAM ATAF1/2 CUC2

NACRS

NAC recognition sequence

ORF

Open reading frame

RT

Reverse transcription

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingChina