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Different expression profiles of jasmonic acid and salicylic acid inducible genes in the tomato plant against herbivores with various feeding modes

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An Erratum to this article was published on 06 January 2016

Abstract

We compared the expression profiles of jasmonic acid (JA)-inducible genes (Pin2 and LapA1) and salicylic acid (SA)-inducible genes (PRb-1b and GluB) in the tomato (Solanum lycopersicum cv. Micro-Tom) against herbivores using differing feeding modes: the leaf-chewing larvae of the insects Spodoptera litura and S. exigua; the western flower thrips (Frankliniella occidentalis) and the two-spotted spider mite (Tetranychus urticae) as cell-content feeders; and the leaf miner fly (Liriomyza sativae). Feeding by larvae of both S. litura and S. exigua chiefly activated JA-inducible genes, similar to the response to wound stimuli. Feeding by the thrips F. occidentalis also activated JA-inducible genes, as previously reported in Arabidopsis. Feeding by the spider mite T. urticae activated a JA-inducible LapA1 gene but did not activate a JA-inducible Pin2 gene and additionally activated SA-inducible genes, which were accompanied by the accumulation of SA. This may be a strain that represses induction of the JA signaling pathway. One day after oviposition by the leaf miner fly, L. sativae, JA-inducible genes were activated. However, after the L. sativae larvae hatched and began eating within the leaf tissues, JA-inducible gene expression decreased and SA-inducible gene expression increased. Activation of SA-inducible genes (PRb-1b and GluB) by L. sativae larval feeding seems to suppress JA-mediated plant defense but appears to be unrelated to SA accumulation.

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Acknowledgments

We thank Ms. Yoko Gotoh and Ms. Masumi Teruse for their technical assistance and Ms. Kayoko Furukawa and Ms. Chiaki Kimoto for their assistance in insect rearing and plant growing. This study was supported in part by the Program for Promotion of Basic Research Activities for Innovative Biosciences, Bio-oriented Technology Research Advancement Institution.

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  1. National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan

    Kei Kawazu, Atsushi Mochizuki, Yukie Sato, Wataru Sugeno & Mika Murata

  2. National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Shigemi Seo & Ichiro Mitsuhara

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  1. Kei Kawazu
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  2. Atsushi Mochizuki
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  3. Yukie Sato
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  4. Wataru Sugeno
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  5. Mika Murata
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  6. Shigemi Seo
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  7. Ichiro Mitsuhara
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Correspondence to Atsushi Mochizuki.

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Handling Editor: Chen-Zhu Wang.

An erratum to this article is available at http://dx.doi.org/10.1007/s11829-015-9412-x.

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11829_2011_9174_MOESM1_ESM.jpg

Online Resources 1 Feeding scars on tomato leaves at different time points after inoculation of chewing caterpillars, Spodoptera litura and S. exigua, and of cell-content feeders, Frankliniella occidentalis and Tetranychus urticae, and after oviposition of leafminer fly, Liriomyza sativae. (JPEG 86 kb)

11829_2011_9174_MOESM2_ESM.jpg

Online Resources 2 Expression profiles of JA- and SA-inducible genes in tomato leaves treated with methyl jasmonate (MeJA), salicylic acid (SA), and distilled water (DW). The data were normalized by the expression level of an Actin gene, and fold-change in the expression levels in the tomato plant at the 4-leaf stage was calculated as a ratio with respect to those of healthy leaves at 0 days. (JPEG 141 kb)

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Kawazu, K., Mochizuki, A., Sato, Y. et al. Different expression profiles of jasmonic acid and salicylic acid inducible genes in the tomato plant against herbivores with various feeding modes. Arthropod-Plant Interactions 6, 221–230 (2012). https://doi.org/10.1007/s11829-011-9174-z

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