Abstract
The aim of this work was to develop a new fat by enzymatic interesterification of mutton tallow with rapeseed oil. It was assumed that by inducing hydrolysis of fats by addition of water to the enzymatic preparation (8, 10, 15 wt%) natural emulsifiers would be produced in the reaction environment. Fat blends obtained from the enzymatic reactions were evaluated as a fat base for emulsion systems. It was found that the fat resulting from interesterification in the presence of Lipozyme RM IM (immobilized lipase from Rhizomucor miehei, Novozymes Bagsvaerd, Denmark) with 15 wt% of water possessed the highest content of polar fraction (MAG and DAG), and served as the most suitable blend for emulsification, producing emulsions that exhibited the highest stability.
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References
Onacik-Gür S, Żbikowska A, Kowalska M (2014) Sources of trans fatty acids on the Polish market. Probl Hig Epidemiol 95:16–23
Nuernberg K, Fischer A, Nuernberg G, Ender K, Dannenberger D (2008) Meat quality and fatty acid composition of lipids in muscle and fatty tissue of Skudde lambs fed grass versus concentrate. Small Ruminant Res 74:279–283
Pieniak-Lendzion K, Nowogródzka T, Horoszewicz E, Niedziółka R (2013) The domestic market lamb against a background of the standards of the European Union. Zeszyty Nauk Uniw Przyrodniczo-Humanistycznego w Siedlcach, Administracja i Zarządzanie 23:19–28
Pivk Kupirovič UN, Godinot Juillerat M, Raspor P (2012) Thickness of lipid deposition on oral surfaces depending on oil content and its influence on mouthfeel perception. Food Technol Biotechnol 50:461–466
Williams C (2000) Dietary fatty acids and human health. Annal Zootech 49:165–180
Lin J, Zhang SM, Cook NR, Lee IM, Buring JE (2004) Dietary fat and fatty acids and risk of colorectal cancer in women. Am J Epidemiol 160:1011–1022
Fritsche J, Steinhart H (1998) Analysis, occurrence and physiological properties of trans fatty acids (TFA) with particular emphasis on conjugated linoleic acid isomers (CLA)—a review. Fett/Lipid 100:190–210
Kritchevsky D, Tepper SA, Wright S, Czarnecki SK, Wilson TA, Nicolosi RJ (2004) Conjugated linoleic acid isomer effects in atherosclerosis: growth and regression of lesions. Lipids 39:611–616
Schmid A, Collomb M, Sieber R, Bee G (2006) Conjugated linoleic acid in meat and meat products: a review. Meat Sci 73:29–41
Kowalska M, Bekas W, Gruczyńska E, Kowalski B (2005) Modification of beef tallow fractions by chemical and enzymatic interesterification with sunflower oil. J Food Technol 3:404–409
Kowalska M, Żbikowska A, Szerling K (2011) Application of modified fats by enzymatic interesterification in emulsions. Italian J Food Sci 23:136–144
Adhikari P, Hu P, Yafei Z (2012) Oxidative stabilities of enzymatically interesterified fats containing conjugated linoleic acid. J Am Oil Chem Soc 89:1961–1970
Costales-Rodríguez R, Gibon V, Verhé R, Greyt WD (2009) Chemical and enzymatic interesterification of a blend of palm stearin: soybean oil for low-trans margarine formulations. J Am Oil Chem Soc 86:681–697
ISO (1996) International Standards Official Methods 660: 1996, animal and vegetable fats and oils—determination of acid value and acidity. ISO, Geneva
ISO (2004) International Standards Official Methods 8420: 2004, animal and vegetable fats and oils—determination of polar compounds content. ISO, Geneva
ISO (1996) International Standards Official Methods 6886: 1997, animal and vegetable fats and oils—determination of oxidation stability (Accelerated Oxidation Test). ISO, Geneva
ISO (1996) International Standards Official Methods Method 3960:1996, animal and vegetable fats and oils—determination of peroxide value. ISO, Geneva
ISO (2000) International Standards Official Methods Method 6321: 2000, Animal and vegetable fats and oils, determination of the temperature of softening point and of full clarification. ISO, Geneva
PN—93/C-04842, Emulsions. Research methods. Determination of emulsion pH type oil/water (o/w). PKN, Warsaw, Poland (in Polish)
Bobálowa J (2006) Preparation of 1-monoacylglycerols by the reaction of fatty acids with glycidol, PhD thesis, University Tomas Bata in Zlin, Zlin
Akoh CC, Moussata CO (2001) Characterization and oxidative stability of enzymatically produced fish and canola oil-based structured lipids. J Am Oil Chem Soc 78:197–204
Zeb A, Murkovic M (2013) Pro-oxidant effects of β-carotene during thermal oxidation of edible oils. J Am Oil Chem Soc 90:881–889
Bouyer E, Mekhloufi G, Rosilio V, Grossiord JL, Agnely F (2012) Proteins, polysaccharides, and their complexes used as stabilizers for emulsions: alternatives to synthetic surfactants in the pharmaceutical field. Int J Pharmaceutics 436:359–378
Huck-Iriart C, Soledad Álvarez-Cerimedo M, Candal JR, Herrera ML (2011) Structures and stability of lipid emulsions formulated with sodium caseinate. Curr Opin in Colloid & Interface Sci 6:412–420
Coupland JN, McClements DJ (2001) Droplet size determination in food emulsions: comparison of ultrasonic and light scattering methods. J Food Eng 50:117–120
Dickinson E (2001) Milk protein interfacial layers and relationship to the emulsion stability and rheology. Colloids Surf B Biointerfaces 20:197–210
Schubert H, Ax K, Behrend O (2003) Product engineering of dispersed systems. Trends Food Sci Technol 14:9–16
Huang X, Kakuda Y, Cui W (2001) Hydrocolloids in emulsions: particle size distribution and interfacial activity. Food Hydrocolloids 15:533–542
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The authors wish to acknowledge the Warsaw Agricultural University and the University of Technology and Humanities in Radom for financial support.
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Kowalska, M., Żbikowska, A. & Kowalski, B. Enzymatically Modified Fats Based on Mutton Tallow and Rapeseed Oil Suitable for Fatty Emulsions. J Am Oil Chem Soc 91, 1703–1710 (2014). https://doi.org/10.1007/s11746-014-2512-4
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DOI: https://doi.org/10.1007/s11746-014-2512-4