Abstract
The paper focuses on research aimed at creating and testing a new approach to evaluate the processes of aggregation and sedimentation of red blood cells for purpose of its use in clinical laboratory diagnostics. The proposed method is based on photometric analysis of blood sample formed as a sessile drop. The results of clinical approbation of this method are given in the paper. Analysis of the processes occurring in the sample in the form of sessile drop during the process of blood cells sedimentation is described. The results of experimental studies to evaluate the effect of the droplet sample focusing properties on light radiation transmittance are presented. It is shown that this method significantly reduces the sample volume and provides sufficiently high sensitivity to the studied processes.
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References
Aristov AA (2005) A Device for evaluation of biological fluid properties. Patent RF 47526
Aristov AA (2010) A method of determining the dynamics of blood cells sedimentation. Patent RF 2379687
Aristov AA, Zhoglo (Nosova) EV (2014) Estimation of blood clotting in the drip samples using optical methods. Proc Int Conf Mech Eng Autom Control Syst. doi:10.1109/MEACS.2014.6986910
Aristov AA, Evtushenko GS, Ermolovich DG (2008) Micromethod of an estimate of erythrocyte sedimentation rate. Proc SPIE Int Soc Opt Eng. doi:10.1117/12.802305
Borovoi AG, Naats EI, Oppel UG (1998) Scattering of light by a red blood cell. J Biomed Opt 3:364–372. doi:10.1117/1.429883
Clampitt R (2002) Method of measuring erythrocyte sedimentation rate (ESR) or plasma fibrinogen of a blood sample. Patent US 6403328
Fine I, Fikhte B, Shvartsman LD (2000) RBC aggregation assisted light transmission through blood and occlusion oximetry. Proc SPIE 4162:120–129
Jou JM et al (2011) ICSH review of the measurement of the erythrocyte sedimentation rate. Int J Lab Hematol 33:125–132. doi:10.1111/j.1751-553X.2011.01302.x
Kishimori S, Hasebe Y (2002) Method and apparatus for measuring sedimentation rate of sediments in liquid sample. Patent US 6336358
Loyko VA, Berdnik VV (2003) Light scattering in dispersed layers with high concentration of soft particles. Opt Spectrosc 95:800–807. doi:10.1134/1.1628731
McMillan ND et al (2008) Quantitative drop spectroscopy using the drop analyser: theoretical and experimental approach for microvolume applications of non-turbid solutions. Meas Sci Technol 19:19–36. doi:10.1088/0957-0233/19/5/055601
McMillan ND et al (2009) Reliable TLDA-microvolume UV spectroscopy with applications in chemistry and biosciences for microlitre analysis and rapid pipette calibration. J Opt A Pure Appl Opt 11:11–22
Miller KE et al (2000) Review of analytical measurements facilitated by drop formation technology. Talanta 51:921–933
Nosova EV, Aristov AA, Nefedova NS (2015) Photometric method of estimation of coagulation process parameters. In: Proceedings of international conference on micro/nanotechnologies and electron devices (EDM): 16th international conference of young specialists, 2015. pp 586–590. doi:10.1109/EDM.2015.7184611
Ponyavina AN (1998) Selection of optical radiation on scattering in partly dispersed clustered media. J Appl Spectrosc 65:721–733
Rafalsky AS, Aristov AA, Evtushenko GS, Zhoglo EV (2012) A device for studying the scattering properties of fluid droplet samples. Instrum Exp Tech 55:283–287. doi:10.1134/S0020441212010216
Saadeh C (1998) The erythrocyte sedimentation rate: old and new clinical applications. South Med J 91:220–225
Shvartsman LD, Fine I (2003) Optical transmission of blood: effect erythrocyte aggregation. IEEE Trans Biomed Eng 50:1026–1033. doi:10.1109/TBME.2003.814532
Yamaikina IV, Ivashkevich EV (1999) A mathematical model of erythrocyte sedimentation in capillaries. J Eng Phys Thermophys 72:55–61. doi:10.1007/BF02699065
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Aristov, A., Nosova, E. Method of evaluation of process of red blood cell sedimentation based on photometry of droplet samples. Med Biol Eng Comput 55, 621–630 (2017). https://doi.org/10.1007/s11517-016-1542-5
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DOI: https://doi.org/10.1007/s11517-016-1542-5