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Callus induction and regeneration in Wolffia arrhiza (L.) Horkel ex Wimm

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An Erratum to this article was published on 17 September 2014

Abstract

The development of tissue-culture systems in duckweed has been limited to species of the genus Lemna. Here we report on a tissue-culture system (callus induction, callus growth and plant regeneration) for the rootless duckweed Wolffia arrhiza. We developed a two-step procedure for callus induction in Wolffia using Schenk & Hildebrandt (SH) medium containing glucose, mannitol and sorbitol. In the first stage, explants were precultivated in the presence of 5.0 mg l−1 2,4-dichlorophenoxyacetic acid and 0.5 mg l−1 N6-benzyladenine (BA) for 16 weeks. In the second stage, BA was replaced with 12.5 mg l−1 Picloram (PCL) for 4 weeks. The resulting callus could be maintained in vitro for a long time (about 1 year) with a relatively low concentration of PCL (4.0 mg l−1), or used to regenerate whole plants by transferring it to growth regulator-free SH medium. The protocols created for callus induction and regeneration were highly efficient at each stage (with the efficiency of 97 % for callus formation and 78 % for regeneration).

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

N6-benzyladenine

Dicamba:

3,6-dichloro-2-methoxybenzoic acid

PCL (Picloram):

4-amino-3,5,6-trichloropicolinic acid

TDZ (thidiazuron):

1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea

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Authors and Affiliations

  1. All-Russia Research Institute of Agricultural Biotechnology, Moscow, Russia

    Pavel Khvatkov, Mariya Chernobrovkina, Anna Okuneva, Anastasiya Shvedova, Inna Chaban & Sergey Dolgov

  2. Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Puschino, Russia

    Pavel Khvatkov, Mariya Chernobrovkina, Anastasiya Shvedova & Sergey Dolgov

Authors
  1. Pavel Khvatkov
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  2. Mariya Chernobrovkina
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  3. Anna Okuneva
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  4. Anastasiya Shvedova
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  5. Inna Chaban
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  6. Sergey Dolgov
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Corresponding author

Correspondence to Pavel Khvatkov.

Additional information

Callus induction and regeneration cell-culture cycle for Wolffia arrhiza has been created for the first time in the world, which is characterized by high efficiency at each stage.

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Khvatkov, P., Chernobrovkina, M., Okuneva, A. et al. Callus induction and regeneration in Wolffia arrhiza (L.) Horkel ex Wimm . Plant Cell Tiss Organ Cult 120, 263–273 (2015). https://doi.org/10.1007/s11240-014-0603-4

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