Abstract
Bottle gourd (Lagenaria sicerar ia Standl.) has been used as a source of rootstock for grafting watermelon to improve its fruit quality. We report here the development of a bottle gourd with resistance to drought by ectopic expression of the Arabidopsis AVP1 gene that encodes a vacuolar H+-pyrophosphatase. The drought resistance of AVP1-expressing and wild-type plants was assessed by growing plants under drought conditions. After 12 days of water deprivation, both AVP1-expressing and wild-type plants demonstrated reduced growth. After 10 days of re-watering, wild-type plants showed minimal growth while the AVP1-expressing plants resumed rapid growth. Further, AVP1-expressing plants displayed longer primary roots and more robust root systems than wild-type plants.
Similar content being viewed by others
References
Doyle JJ, Dickson EE (1987) Preservation of plant samples for DNA restriction endonuclease analysis. Taxon 36:715–772
Gaxiola RA, Fink GR, Hirschi KD (2002) Genetic manipulation of vascular proton pumps and transporters. Plant Physiol 129:967–973
Gaxiola RA, Sanchez CA, Paez-Valencia J, Ayre BG, Elser JJ (2012) Genetic manipulation of a “Vacuolar” H+-PPase: from salt tolerance to yield enhancement under phosphorus-deficient soils. Plant Physiol 159:1–9
Gonzalez N, Beemster GTS, Inze D (2009) David and Goliath: what can the tiny weed Arabidopsis teach us to improve biomass production in crops? Curr Opin Plant Biol 12:157–164
Gonzalez N, De Bodt S, Sulpice R, Jikumaru Y, Chae E, Dhondt S et al (2010) Increased leaf size: different means to an end. Plant Physiol 153:1261–1279
Han JS, Oh DG, Mok IG, Park HG, Kim CK (2004) Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.). Plant Cell Rep 23:291–296
Han JS, Kim CK, Park SH, Hirschi KD, Mok IG (2005) Agrobacterium-mediated transformation of bottle gourd (Lagenaria siceraria Standl.). Plant Cell Rep 23:692–698
Han JS, Park S, Shigaki T, Hirschi KD, Kim CK (2009) Improved watermelon quality using bottle gourd rootstock expressing a Ca2+/H+ antiporter. Mol Breed 24:201–211
Kubota C, McClure MA, Kokalis-Burelle N, Bausher MG, Rosskopf EN (2008) Vegetable grafting: history, use, and current technology status in North America. HortScience 43:1664–1669
Lee J, Oda M (2003) Grafting of herbaceous vegetable and ornamental crops. Hortic Rev 28:61–124
Li J, Yang H, Peer WA, Richer G, Blackesless J, Bandyopadhyay A, Titapiwantakun B, Undurraga S, Khodakovskaya M, Richards EL, Krizek B, Murphy AS, Gilroy S, Gaxiola R (2005) Arabidopsis H+-PPase AVP1 regulates auxin-mediated organ development. Science 310:121–125
Li B, Wei A, Song C, Li N, Zhang J (2008) Heterologous expression of the TsVP gene improves the drought resistance of maize. Plant Biotech J 6:146–159
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta C(T)) method. Methods 25:402–408
Lv SL, Zhang K, Gao Q, Lian L, Song Y, Zhang JR (2008) Overexpression of an H+-PPase gene from Thellungiella halophila in cotton enhances salt tolerance and improves growth and photosynthetic performance. Plant Cell Physiol 49:1150–1164
Lv S, Lian L, Tao P, Li Z, Zhang K, Zhang J (2009) Overexpression of Thellungiella halophila H+-PPase (TsVP) in cotton enhances drought stress resistance of plants. Planta 229:899–910
Murashige T, Skoog FA (1962) Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Paez-Valencia J, Sanchez-Lares J, Marsh E, Dorneles LT, Santos M, Sanchez D, Winter A, Murphy S, Cox J, Trzaska M, Metler T, Kozic A, Facanha A, Schachtma D, Sanchez C, Gaxiola RA (2013) Enhanced proton translocating pyrophosphatase activity improves nitrogen use efficiency in romaine lettuce. Plant Physiol 161:1557–1569
Park S, Li J, Pittman JK, Berkowitz GA, Yang H, Undurraga S, Morris J, Hirschi KD, Gaxiola RA (2005a) Up-regulation of a H-pyrophosphatase (H-PPase) as a strategy to engineer drought-resistant crop plants. Proc Natl Acad Sci 102:18830–18835
Park SM, Lee JS, Jegal S, Jeon BY, Jung M, Park YS et al (2005b) Transgenic watermelon rootstock resistant to CGMMV(cucumber green mottle mosaic virus) infection. Plant Cell Rep 24:350–356
Park M, Won H, Kim CK, Han JS (2012) Increased biomass and enhanced tolerance to salt stress in Chinese cabbage overexpressing Arabidopsis H+-PPase (AVP1). J Plant Biotechnol 39:253–260
Pasapula V, Shen G, Kuppu S, Paez-Valencia J, Mendoza M, Hou P et al (2011) Expression of an Arabidopsis vacuolar H+-pyrophosphatase gene (AVP1) in cotton improves drought and salt tolerance and increases fibre yield in the field conditions. Plant Biotech J 9:88–99
Qin H, Gu Q, Kuppu S, Sun L, Zhu X, Mishra N, Hu R, Shen G, Zhang J, Zhang Y, Zhu L, Zhang X, Burow M, Payton P, Zhang H (2012) Expression of the Arabidopsis vacuolar H+-pyrophosphatase gene AVP1 in peanut to improve drought and salt tolerance. Plant Biotechnol Rep. doi:10.1007/s11816-012-0269-5
Samac DA, Tesfaye M, Dornbusch M, Saruul P, Temple SJ (2004) A comparison of constitutive promoters for expression of transgenes in alfalfa (Medicago sativa). Transgenic Res 13:349–361
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
Sunilkumar G, Mohr L, Lopata-Finch E, Emani C, Rathore KS (2002) Developmental and tissue-specific expression of CaMV 35S promoter in cotton as revealed by GFP. Plant Mol Biol 50:463–474
Yang H, Knapp J, Koirala P, Rajagopal D, Peer WA, Silbart LK et al (2007) Enhanced phosphorus nutrition in monocots and dicots over-expressing a phosphorus-responsive type I H+-pyrophosphatase. Plant Biotech J 5:735–745
Zhao F, Zhang X, Li P, Zhao Y, Zhang H (2006) Co-expression of Suaeda salsa SsNHX1 and Arabidopsis AVP1 confer greater salt tolerance to transgenic rice than the single SsNHX1. Mol Breed 17:341–353
Zhen RG, Kim EJ, Rea PA (1997) Acidic residues necessary for pyrophosphate-energized pumping and inhibition of the vacuolar H+-pyrophosphatase by N,N’-dicyclohexylcarbodiimide. J Biol Chem 272:22340–22348
Acknowledgments
This research was supported by the NIHHS RDA-KSU Cooperative Research Project and a grant from the RDA (PJJ00863802013).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Park, M., Han, J., Ahn, Y. et al. Ectopic expression of Arabidopsis H+-pyrophosphatase AVP1 enhances drought resistance in bottle gourd (Lagenaria siceraria Standl.). Plant Cell Tiss Organ Cult 118, 383–389 (2014). https://doi.org/10.1007/s11240-014-0490-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11240-014-0490-8