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Ectopic expression of Arabidopsis H+-pyrophosphatase AVP1 enhances drought resistance in bottle gourd (Lagenaria siceraria Standl.)

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Abstract

Bottle gourd (Lagenaria sicerar ia Standl.) has been used as a source of rootstock for grafting watermelon to improve its fruit quality. We report here the development of a bottle gourd with resistance to drought by ectopic expression of the Arabidopsis AVP1 gene that encodes a vacuolar H+-pyrophosphatase. The drought resistance of AVP1-expressing and wild-type plants was assessed by growing plants under drought conditions. After 12 days of water deprivation, both AVP1-expressing and wild-type plants demonstrated reduced growth. After 10 days of re-watering, wild-type plants showed minimal growth while the AVP1-expressing plants resumed rapid growth. Further, AVP1-expressing plants displayed longer primary roots and more robust root systems than wild-type plants.

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Acknowledgments

This research was supported by the NIHHS RDA-KSU Cooperative Research Project and a grant from the RDA (PJJ00863802013).

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Correspondence to Sunghun Park.

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Park, M., Han, J., Ahn, Y. et al. Ectopic expression of Arabidopsis H+-pyrophosphatase AVP1 enhances drought resistance in bottle gourd (Lagenaria siceraria Standl.). Plant Cell Tiss Organ Cult 118, 383–389 (2014). https://doi.org/10.1007/s11240-014-0490-8

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  • DOI: https://doi.org/10.1007/s11240-014-0490-8

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