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A wheat superoxide dismutase gene TaSOD2 enhances salt resistance through modulating redox homeostasis by promoting NADPH oxidase activity

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Abstract

Superoxide dismutase (SOD) is believed to enhance abiotic stress resistance by converting superoxide radical (O2 −) to H2O2 to lower ROS level and maintain redox homeostasis. ROS level is controlled via biphasic machinery of ROS production and scavenging. However, whether the role of SOD in abiotic stress resistance is achieved through influencing the biophasic machinery is not well documented. Here, we identified a wheat copper-zinc (Cu/Zn) SOD gene, TaSOD2, who was responsive to NaCl and H2O2. TaSOD2 overexpression in wheat and Arabidopsis elevated SOD activities, and enhanced the resistance to salt and oxidative stress. TaSOD2 overexpression reduced H2O2 level but accelerated O2 − accumulation. Further, it improved the activities of H2O2 metabolic enzymes, elevated the activity of O2 − producer NADPH oxidase (NOX), and promoted the transcription of NOX encoding genes. The inhibition of NOX activity and the mutation of NOX encoding genes both abolished the salt resistance of TaSOD2 overexpression lines. These data indicate that Cu/Zn SOD enhances salt resistance, which is accomplished through modulating redox homeostasis via promoting NOX activity.

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Acknowledgments

This work was supported by the National Transgenic Project (2014ZX08002002-003), and the Natural Science Foundation of China (31171175).

Authors contributions

Mengcheng Wang and Guangmin Xia designed the work. Mengcheng Wang, Xin Zhao, Zhen Xiao, Xunhao Yin and Tian Xing conducted the experiments. Mengcheng Wang and Guangmin Xia wrote the paper.

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Authors and Affiliations

  1. The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shandanan Road, Jinan, 250100, Shandong, China

    Mengcheng Wang, Xin Zhao, Zhen Xiao, Xunhao Yin, Tian Xing & Guangmin Xia

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  1. Mengcheng Wang
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  2. Xin Zhao
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  3. Zhen Xiao
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  4. Xunhao Yin
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  5. Tian Xing
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  6. Guangmin Xia
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Corresponding author

Correspondence to Guangmin Xia.

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TaSOD2 asccession: KP322572.

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Wang, M., Zhao, X., Xiao, Z. et al. A wheat superoxide dismutase gene TaSOD2 enhances salt resistance through modulating redox homeostasis by promoting NADPH oxidase activity. Plant Mol Biol 91, 115–130 (2016). https://doi.org/10.1007/s11103-016-0446-y

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