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Seed-specific increased expression of 2S albumin promoter of sesame qualifies it as a useful genetic tool for fatty acid metabolic engineering and related transgenic intervention in sesame and other oil seed crops

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Abstract

The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin promoter. Prediction based deletion analysis of both the promoter elements confirmed the necessary cis-acting regulatory elements as well as the minimal promoter element for optimal expression in each case. The results also revealed that cis-regulatory elements might have been responsible for high level expression as well as spatio-temporal regulation of the sesame 2S albumin promoter. Transgenic over-expression of a fatty acid desaturase (fad3C) gene of soybean driven by 2S albumin promoter resulted in seed-specific enhanced level of α-linolenic acid in sesame. The present study, for the first time helped to identify that the sesame 2S albumin promoter is a promising endogenous genetic element in genetic engineering approaches requiring spatio-temporal regulation of gene(s) of interest in sesame and can also be useful as a heterologous genetic element in other important oil seed crop plants in general for which seed oil is the harvested product. The study also established the feasibility of fatty acid metabolic engineering strategy undertaken to improve quality of edible seed oil in sesame using the 2S albumin promoter as regulatory element.

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Acknowledgments

The authors offer their appreciation for Gayatri Aditya and Sona Dogra for their technical assistance during the course of this study, Natasha Das for her help in making sections of plant tissues, and Meghnath Prasad for secretarial assistance in preparing this report. Financial assistance from Indian Council of Agricultural Research (NAIP/ICAR) in terms of grant support (Project component code 4C1090) to laboratory and fellowship to RKB is thankfully acknowledged. Finally, the authors extend their grateful appreciation for two anonymous reviewers whose Comments and suggestions have helped immensely to improve the quality of the manuscript.

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Authors and Affiliations

  1. Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, Kharagpur, 721302, India

    Rupam Kumar Bhunia, Anirban Chakraborty, Ranjeet Kaur, T. Gayatri, Jagannath Bhattacharyya, Asitava Basu, Mrinal K. Maiti & Soumitra Kumar Sen

  2. Department of Biotechnology, Indian Institute of Technology, Kharagpur, 721302, India

    Mrinal K. Maiti

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  1. Rupam Kumar Bhunia
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  2. Anirban Chakraborty
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  4. T. Gayatri
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Correspondence to Soumitra Kumar Sen.

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Ranjeet Kaur and T. Gayatri have contributed equally to this work.

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Bhunia, R.K., Chakraborty, A., Kaur, R. et al. Seed-specific increased expression of 2S albumin promoter of sesame qualifies it as a useful genetic tool for fatty acid metabolic engineering and related transgenic intervention in sesame and other oil seed crops. Plant Mol Biol 86, 351–365 (2014). https://doi.org/10.1007/s11103-014-0233-6

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