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Fluorescent Dye Labeled Iron Oxide/Silica Core/Shell Nanoparticle as a Multimodal Imaging Probe

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An Erratum to this article was published on 02 December 2014

Abstract

Purpose

To develop an MRI/optical multimodal imaging probe based on dye-conjugated iron oxide/silica core/shell nanoparticle, and investigate the distance-dependent fluorescence quenching through careful control of the distance between the iron oxide core and fluorescent dyes.

Methods

Different size of core/shell nanoparticles were prepared by varying the silica shell width. PEGylation on the surface of silica shell was followed to improve the stability of particles in the physiological condition. In vitro cytotoxicity was evaluated by the MTT assay on a HeLa cell line and in vivo imaging of subcutaneous SCC7 xenografted mice was performed using MRI/optical imaging modalities.

Results

Diameter and ζ-potential of the nanoparticles were measured, and TEM images demonstrated the mono-disperse nature of the particles. Quenching efficiency of the dyes on the surface was nearly 100% in the smallest nanoparticle, while almost no quenching effect was observed for the largest nanoparticle. In vitro cytotoxicity showed nearly 90% cell viability at 0.15 Fe mg/mL, a comparable concentration for clinical use. The tumor area was significantly darkened after the nanoparticle injection due to the high transverse relaxivity value of the nanoparticles. Fluorescence signal was affected by the particle size due to the distance-dependent quenching/dequenching behaviour.

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Abbreviations

APTES:

3-aminopropyl triethoxysilane

Cy:

Cyanine

CY-CS:

Cy-conjugated CS

CS:

Core-shell

DMEM:

Dulbecco’s modified eagle medium

EDC:

Ethyl (dimethylaminopropyl) carbodiimide

FBS:

Fetal bovine serum

FRET:

Fluorescence resonance energy transfer

HF:

Hydrofluoric acid

ICP-MS:

Inductively coupled plasma mass spectrometry

mPEG-COOH:

Methoxy poly (ethylene glycol) mono acid

MRI:

Magnetic resonance imaging

MTT:

Methylthiazol tetrazolium

NIRF:

Near-infrared fluorescence

NHS:

N-hydroxysuccinimide

PL:

Photoluminescence

TEM:

Transmission electron microscopy

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Acknowledgments and Disclosures

This study was supported by a grant from Basic Science Research Program (grant no. 2010–0027955) of MEST and National Research Foundation of Korea (grant no. 2010–0023581). C.-H. Ahn appreciate LG Yonam Foundation for the support.

Author information

Authors and Affiliations

  1. Advanced Materials Educational Institute, Kumoh National Institute of Technology, Sanho-ro 77, Yangho, Gumi, 730-701, South Korea

    Eue Soon Jang

  2. Research Institute of Advanced Materials (RIAM), Department of Materials Science and Engineering, Seoul National University, San 56-1, Sillim, Gwanak, Seoul, 151-744, South Korea

    Seung Yong Lee, Eui-Joon Cha & Cheol-Hee Ahn

  3. Biomedical Research Center, Korea Institute of Science and Technology (KIST), 39-1, Hawolgok, Seongbuk, Seoul, 136-791, South Korea

    In-Cheol Sun & Kwangmeyung Kim

  4. Department of Chemical Engineering, Theranostic Macromolecule Research Center, Sungkyunkwan University, Suwon, Kyunggi, 440-746, South Korea

    Ick Chan Kwon

  5. Department of Radiology, Seoul National University Hospital, 28, Yeongeon, Jongno, Seoul, 110-744, South Korea

    Dukjoon Kim & Young Il Kim

  6. Materials Science and Engineering, Seoul National University, Seoul, 151-744, South Korea

    Cheol-Hee Ahn

  7. Biomedical Research Center, Korea Institute of Science and Technology, Seoul, 136-791, South Korea

    Kwangmeyung Kim

  8. Radiology, Seoul National University Hospital, Seoul, 110-744, South Korea

    Young Il Kim

Authors
  1. Eue Soon Jang
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  2. Seung Yong Lee
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  7. Young Il Kim
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  9. Cheol-Hee Ahn
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Corresponding authors

Correspondence to Young Il Kim, Kwangmeyung Kim or Cheol-Hee Ahn.

Additional information

These authors Eue Soon Jang and Seung Yong Lee are contributed equally to this paper

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Jang, E.S., Lee, S.Y., Cha, EJ. et al. Fluorescent Dye Labeled Iron Oxide/Silica Core/Shell Nanoparticle as a Multimodal Imaging Probe. Pharm Res 31, 3371–3378 (2014). https://doi.org/10.1007/s11095-014-1426-z

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  • DOI: https://doi.org/10.1007/s11095-014-1426-z

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