Abstract
Jute is the most important bast fibre crop of the world. Stem rot disease caused by Macrophomina phaseolina is a major constraint of jute cultivation. Recent advances in genomics and bioinformatics have enabled the development of high-throughput genotyping, allowing the construction of saturated linkage maps for identification of resistant lines. In the present study, single nucleotide polymorphism (SNP) markers associated with expressed sequence tags (ESTs) were developed and were used to generate comprehensive linkage maps for Corchorus capsularis. From a set of 43,335 variant nucleotide positions detected through in silico analysis, 768 SNPs were selected for genotyping of a recombinant inbred line population. A total of 705 SNPs (91.7 %) successfully detected segregating polymorphisms. In addition to EST–SNPs, genomic simple sequence repeats were assigned to the genetic map in order to obtain an evenly distributed genome-wide coverage. Nine linkage groups were constructed from genetic maps with estimated cumulative total map length of 2016 cM and average inter-locus interval of 4.2 cM. SNP assay and linkage mapping are important set of tools for marker-assisted selection, and it could be used for identifying resistance to M. phaseolina in jute cultivars. This is the first report of developing SNP markers and SNP-based linkage map in jute.
Similar content being viewed by others
References
Barker GL, Edwards KJ (2009) A genome-wide analysis of single nucleotide polymorphism diversity in the world’s major cereal crops. Plant Biotechnol J 7:318–325
Biswas C, Singh D, Singh R, Kumar V (2009) Incidence of bacterial blight and Alternaria leaf spot in Bt cotton. Indian Phytopathol 62(2):256–257
Biswas C, Sarkar SK, De RK (2011) Diseases of jute and mesta: present status and management options. In: Biswas SK, Singh SR (eds) Sustainable disease management of agricultural crops. Daya Publishing House, New Delhi, pp 62–79
Biswas C, Dey P, Satpathy S, Sarkar SK, Bera A, Mahapatra BS (2013) A simple method of DNA isolation from jute (Corchorus olitorius) seed suitable for PCR based detection of the pathogen Macrophomina phaseolina (Tassi) Goid. Lett Appl Microbiol 56:105–110
Biswas C, Dey P, Karmakar PG, Satpathy S (2014) Next-generation sequencing and micro RNAs analysis reveal SA/MeJA1/ABA pathway genes mediated Systemic Acquired Resistance (SAR) and its master regulation via production of phased, trans-acting siRNAs against stem rot pathogen Macrophomina phaseolina in a RIL population of jute (Corchorus capsularis). Physiol Mol Plant Pathol 87:1–10
Brown DG, Vision TJ (2000) MapPop version 1.0: Software for selective mapping and bin mapping. 2000. http://www.bio.unc.edu/faculty/vision/lab/mappop/
Choi HK, Kim DJ, Uhm T, Limpens E, Lim H, Mun JH, Kalo P, Penmetsa RV, Seres A, Kulikova O, Roe BA, Bisseling T, Kiss GB, Cook DR (2004) A sequence- based genetic map of Medicago truncatula and comparison of marker colinearity with M. sativa. Genetics 166:1463–1502
Das M, Banerjee S, Dhariwal R, Vyas S, Mir RR, Topdar N, Kundu A, Khurana JP, Tyagi AK, Sarkar D, Sinha MK, Balyan HS, Gupta PK (2012) Development of SSR markers and construction of a linkage map in jute. J Genet 91(1):21–31
Deleu W, Esteras C, Roig C, Gonzalez-To M, Fernandez-Silva I, Gonzalez-Ibeas D, Blanca J, Aranda MA, Arus P, Nuez F, Monforte AJ, Pico MB, Garica-Mas J (2009) A set of EST-SNPs for map saturation and cultivar identification in melon. BMC Plant Biol 9:90
Ghosh T (1983) Handbook on Jute. FAO, Rome, p 219
Gordon D, Abajian C, Green P (1998) Consed: a graphical tool for sequence finishing. Genome Res 8(3):195–202
Gupta PK, Rustgi S, Mir RR (2008) Array-based high throughput DNA markers for crop improvement. Heredity 101:5–18
Holliday R, Grigg GW (1993) DNA methylation and mutation. Mutation 285:61–67
Islam AS, Taliaferro M, Lee CT, Ingram CI, Montalvo RJ, Van der Ende G, Alam S, Siddiqui J, Sathasivan K (2005) Preliminary progress in jute (Corchorus species) genome analysis. Plant Tissue Cult Biotechnol 15:145–156
Kosambi DD (1944) The estimation of map distance from recombination values. Ann of Eugen 12:172–175
Kundu BC (1956) Jute—world’s foremost bast fibre, II. Technology, marketing, production and utilization. Econ Bot 10(3):203–240
Liu G, Bernhardt JL, Jia MH, Wamishe YA, Jia Y (2008) Molecular characterization of the recombinant inbred line population derived from a japonica indica rice cross. Euphytica 159:73–82
Mammadov J, Aggarwal R, Buyyarapu R, Kumpatla S (2012a) SNP markers and their impact on plant breeding. Int J Plant Genomics 728398:1–11
Mammadov J, Chen W, Mingus J, Thompson S, Kumpatla S (2012b) Development of versatile gene-based SNP assays in maize (Zea mays L.). Mol Breed 29:779–790
Manly KF, Cudmore RH Jr, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932
Marth GT, Korf I, Yandell MD, Yeh RT, Gu Z, Zakeri H, Stitziel NO, Hillier L, Kwok PY, Gish WR (1999) A general approach to single-nucleotide polymorphism discovery. Nat Genet 23(4):452–456
Mir RR, Rustgi S, Sharma S, Singh R, Goyal A, Kumar J, Gaur A, Tyagi AK, Khan H, Sinha MK, Balyan HS, Gupta PK (2008) A preliminary genetic analysis for fibre traits and the use of new genomic SSRs for genetic diversity in jute. Euphytica 16:413–427
Mir RR, Banerjee S, Das M, Gupta V, Tyagi AK, Sinha MK, Balyan HS, Gupta PK (2009) Development and characterization of large scale simple sequence repeats in jute. Crop Sci 49:1687–1694
Powell W, Machray GC, Proven J (1996) Polymorphism revealed by simple sequence repeats. Trends Plant Sci 1(3):215–222
Roy A, De RK, Ghosh SK (2008) Diseases of bast fibre crops and their management. In: Karmakar PG, Hazra SK, Ramasubramanian T, Mandal RK, Sinha MK, Sen HS (eds) Jute and allied fibre updates. Central Research Institute for Jute and Allied Fibres, Kolkata, pp 217–241
Said JI, Lin Z, Zhang X, Song M, Zha J (2013) A comprehensive meta QTL analysis for fiber quality, yield, yield related and morphological traits, drought tolerance, and disease resistance in tetraploid cotton. BMC Genom 14:776. doi:10.1186/1471-2164-14-776
Sarkar D, Kundu A, Saha A, Mondal NA, Sinha MK, Mahapatra BS (2011) First nuclear DNA amounts in diploid (2n = 2x = 14) Corchorus spp. by flow cytometry: genome sizes in the cultivated jute species (C. capsularis L. and C. olitorius L.) are ~ 300% smaller than the reported estimate of 1100-1350 Mb. Caryologia 64(2):147–153
Shen X, He Y, Lubbers EL, Davis RF, Nichols RL, Chee PW (2010) Fine mapping QMi-C11 a major QTL controlling root-knot nematodes resistance in Upland cotton. Theor Appl Genet 121(8):1623–1631
Sim SC, Durstewitz G, Plieske J, Wieseke R, Ganal MW, Van Deynze A, Hamilton JP, Buell CR, Causse M, Wijeratne S, Francis DM (2012) Development of a large SNP genotyping array and generation of high-density genetic maps in tomato. PLoS ONE 7:e40563
Topdar N, Kundu A, Sinha MK, Sarkar D, Das M, Banerjee S, Kar CS, Satya P, Balyan HS, Mahapatra BS, Gupta PK (2013) A complete genetic linkage map and QTL analysis for bast fibre quality traits, yield and yield components in jute (Corchorus olitorius L.). Cytol Genet 47:129–137
van Ooijen J, Voorrips R (2001) JoinMap 3.0 software for the calculation of genetic linkage maps. Plant Research International, Wageningen
Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78
Acknowledgments
We acknowledge the Xcelris Labs Ltd., Ahmedabad, for assistance in SNP detection and analysis work. The research was funded by Indian Council of Agricultural Research (ICAR), New Delhi. Comments and suggestions on the manuscript from three anonymous reviewers are gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Biswas, C., Dey, P., Karmakar, P.G. et al. Discovery of large-scale SNP markers and construction of linkage map in a RIL population of jute (Corchorus capsularis). Mol Breeding 35, 119 (2015). https://doi.org/10.1007/s11032-015-0311-8
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s11032-015-0311-8