Skip to main content
SpringerLink
Log in

Nanostructured manganese oxide–chitosan-based cholesterol sensor

  • Research Article
  • Published:
Journal of Applied Electrochemistry Aims and scope Submit manuscript

Abstract

Manganese oxide nano-rod (nano-MnO2) was prepared by simple oxidation method. To fabricate nanocomposite film (nano-MnO2/CT) onto glassy carbon electrode (GCE), nano-MnO2 was homogeneously dispersed in chitosan (CT). Cholesterol oxidase (ChOx) was immobilized onto nano-MnO2/CT by physisorption. Modified electrode was characterized by Fourier transform infrared, X-ray diffraction, cyclic voltammetry, scanning electron microscopy, transmission electron microscopy, and electrochemical impedance spectroscopy techniques. Prepared ChOx/nano-MnO2/CT/GCE bioelectrode exhibited 0.03–11.66 mM linearity and 2.07 × 10−3 mM limit of detection for cholesterol. Biosensing characteristics of modified bioelectrode were superior than other electrodes modified with metal oxide nanoparticles, reported in the literature.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Scheme 1
Fig. 10
Fig. 11
Fig. 12
Fig. 13

Similar content being viewed by others

References

  1. Baynes JW, Dominiczak M (2005) Medical biochemistry, 2nd edn. Elsevier, Amsterdam

    Google Scholar 

  2. White A, Handler P, Smith EL, Hill RL, Lehman IR (1987) Principles of biochemistry, 6th edn. McGraw-Hill Book, New York, pp 903–907

    Google Scholar 

  3. Kumar A, Pandey RR, Brantley B (2006) Talanta 69:700–705

    Article  CAS  Google Scholar 

  4. Khan R, Kaushik A, Solanki PR, Ansari AA, Pandey MK, Malhotra BD (2008) Anal Chim Acta 616:207–213

    Article  CAS  Google Scholar 

  5. Ansari AA, Kaushik A, Solanki PR, Malhotra BD (2008) Electrochem Commun 10:1246–1249

    Article  CAS  Google Scholar 

  6. Singh SP, Arya SK, Pandey P, Malhotra BD, Saha S, Sreenivas K, Gupta V (2007) Appl Phys Lett 91:063901–063903

    Article  Google Scholar 

  7. Malhotra BD, Kaushik A (2009) Thin Solid Films 518:614–620

    Article  CAS  Google Scholar 

  8. Jiang HL, Kwon JT, Kim YK, Kim EM, Arote R, Jeong HJ, Nah JW, Choi YJ, Akaike T, Cho MH, Cho CS (2007) Gene Ther 14:1389–1398

    Article  CAS  Google Scholar 

  9. Liao JD, Lin SP, Wu YT (2005) Biomacromolecules 6:392–399

    Article  CAS  Google Scholar 

  10. Suib SL (2008) Acc Chem Res 41:479–487

    Article  CAS  Google Scholar 

  11. Kuo C-C, Lan W-J, Chen C-H (2014) Nanoscale 6:334–341

    Article  CAS  Google Scholar 

  12. Liu A (2008) Biosens Bioelectron 24:167–177

    Article  CAS  Google Scholar 

  13. Zhai T, Fang X, Liao M, Xu X, Zeng H, Yoshio B, Golberg D (2009) Sensors 9:6504–6529

    Article  CAS  Google Scholar 

  14. Huang J, Wan Q (2009) Sensors 9:9903–9924

    Article  Google Scholar 

  15. Ahammad AJS, Lee JJ, Rahman MA (2009) Sensors 9:2289–2319

    Article  CAS  Google Scholar 

  16. Kaushik A, Khan R, Solanki PR, Pandey P, Ahmad S, Malhotra BD (2008) Biosens Bioelectron 24:676–683

    Article  CAS  Google Scholar 

  17. Wei H, Wang E (2008) Anal Chem 80:2250–2254

    Article  CAS  Google Scholar 

  18. Jia NQ, Zhou Q, Liu L, Yan MM, Jiang ZY (2005) J Electroanal Chem 580:213–221

    Article  CAS  Google Scholar 

  19. Yu J, Ju H (2002) Anal Chem 74:3579–3583

    Article  CAS  Google Scholar 

  20. Kim HJ, Yoon SH, Choi HN, Lyu YK, Lee WY (2006) Bull Korean Chem Soc 27:65–70

    Article  CAS  Google Scholar 

  21. Ansari AA, Solanki PR, Malhotra BD (2008) Appl Phys Lett 92:263901–263903

    Article  Google Scholar 

  22. Mehta A, Patil S, Bang H, Cho HJ, Seal S (2007) Sens Actuators. A 134:146–151

    CAS  Google Scholar 

  23. Singh J, Kalita P, Singh MK, Malhotra BD (2011) Appl Phys Lett 98:123702–123703

    Article  Google Scholar 

  24. Tsai YC, Chen SY, Lee CA (2008) Sens Actuators. B Chem 135:96–101

    CAS  Google Scholar 

  25. Tiwari A, Gong S (2008) Electroanalysis 20:2119–2126

    Article  CAS  Google Scholar 

  26. Safavi A, Farjami F (2011) Biosens Bioelectro 26:2547–2552

    Article  CAS  Google Scholar 

  27. Ansari AA, Kaushik A, Solanki PR, Malhotra BD (2009) Electroanalysis 21:965–972

    Article  CAS  Google Scholar 

  28. Kaushik A, Solanki PR, Kaneto K, Kim CG, Ahmad S, Malhotra BD (2010) Electroanalysis 22:1045–1055

    Article  CAS  Google Scholar 

  29. Norouzi P, Faridbod F, Nasli-Esfahani E, Larijani B, Ganjali MR (2010) Int J Electrochem Sci 5:1008–1017

    CAS  Google Scholar 

  30. Report of the National Cholesterol Education Program Expert Panel on detection, evaluation, and treatment of high blood cholesterol in adults (1988). Arch Intern Med 148:36–69

  31. Choi SH, Lee SD, Shin JH, Ha J, Nam H, Cha GS (2002) Anal Chim Acta 461:251–260

    Article  CAS  Google Scholar 

  32. Zhu S, Zhou H, Hibino M, Honma I, Ichihara M (2005) Adv Funct Mater 15:381–386

    Article  CAS  Google Scholar 

  33. Khan R, Dhayal M (2008) Electrochem Commun 10:263–267

    Article  CAS  Google Scholar 

  34. Wang X, Li Y (2003) Chem Eur J 9:300–306

    Article  Google Scholar 

  35. Zhang HL, Zou XZ, Lai GS, Han DY, Wang F (2007) Electroanalysis 19:1869–1874

    Article  CAS  Google Scholar 

  36. Bard AJ, Faulkner LR (2000) Electrochemical methods: fundamentals and applications, 2nd edn. Wiley, New York

    Google Scholar 

  37. Tan X, Li M, Cai P, Luo L, Zou X (2005) Anal Biochem 337:111–120

    Article  CAS  Google Scholar 

  38. Basu A, Chattopadhyay P, Roychoudhuri U, Chakraborty R (2007) Bioelectrochemistry 70:375–379

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Authors are thankful to Department of Science and Technology (DST) for financial support by sponsoring project no. DST/TSG/ME/2010/57. We also acknowledge the services of analytical science division, CSMCRI, Bhavnagar for instrumental support.

Author information

Authors and Affiliations

  1. Academy of Scientific and Innovative Research, New Delhi, India

    Chumki Charan & Vinod K. Shahi

  2. Electro-Membrane Processes Division, CSIR-Central Salt and Marine Chemicals Research Institute, G.B. Marg, Bhavnagar, 364002, Gujarat, India

    Chumki Charan & Vinod K. Shahi

Authors
  1. Chumki Charan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vinod K. Shahi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vinod K. Shahi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Charan, C., Shahi, V.K. Nanostructured manganese oxide–chitosan-based cholesterol sensor. J Appl Electrochem 44, 953–962 (2014). https://doi.org/10.1007/s10800-014-0704-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10800-014-0704-0

Keywords

Search

Navigation