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Assessment of antioxidant defense system responses in the hepatopancreas of the freshwater crab Sinopotamon henanense exposed to lead

  • CHALLENGES IN AQUATIC SCIENCES
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Abstract

Lead (Pb) is a common pollutant in aquatic ecosystems, which produces a wide range of toxic biochemical effects in different organisms. The aim of the present study was to explore the mechanisms of acute toxicity and antioxidant defenses in freshwater crab Sinopotamon henanense induced by Pb. Hepatopancreas was collected from S. henanense exposed for 96 h to Pb (0, 9.188, 18.375, 36.75, 73.5, and 147 mg/l). Oxidative stress was examined using a suite of assays in crabs, including contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA), activities of antioxidative enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), and glutathione system-related parameters such as glutathione (GSH), glutathione S-transferase (GST), and glutathione reductase (GR). A dose-dependent increase of H2O2 and MDA was observed in the crabs after Pb exposure, while antioxidative enzymes activities were suppressed significantly (P < 0.05) at higher concentrations of Pb. The ratios of CAT/SOD, GPx/SOD, and GR/GPx were also suppressed. Our results suggested that acute exposure of Pb causes lipid peroxidation and harmful lessened antioxidant defenses of crabs. The above parameters were evaluated as potential biomarkers for Pb pollution monitoring and health assessment of crabs which is also important for the aquaculture of crabs.

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Acknowledgments

We thank all members of our lab for their help in this project, and we are thankful to Dr. Hans U. Dahms for providing suggestions and language help. The work was supported by Higher Specialized Research Fund for the Doctoral Program Jointly Funded Project (Doctoral category) (No. 20111401110010, to Lan Wang) and Basic Research Program Youth Project of Shanxi Province (No. 2013021022-4, to Na Liu).

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  1. School of Life Science, Shanxi University, Wucheng Road 92, Taiyuan, 030006, China

    Na Liu, Lan Wang, Bo Yan, Yongquan Li, Fei Ye, Jing Li & Qian Wang

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  1. Na Liu
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  2. Lan Wang
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  3. Bo Yan
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  4. Yongquan Li
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Correspondence to Lan Wang.

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Guest editors: Jiang-Shiou Hwang & Koen Martens /Challenges in Aquatic Sciences

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Liu, N., Wang, L., Yan, B. et al. Assessment of antioxidant defense system responses in the hepatopancreas of the freshwater crab Sinopotamon henanense exposed to lead. Hydrobiologia 741, 3–12 (2014). https://doi.org/10.1007/s10750-014-1806-8

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  • DOI: https://doi.org/10.1007/s10750-014-1806-8

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