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Mapping QTLs related to rice seed storability under natural and artificial aging storage conditions

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Abstract

Seed aging or deterioration in rice (Oryza sativa L.) is a major problem for agronomic production and germplasm preservation. Deciphering the genetic mechanism involved in seed aging and improving seed storability is therefore a vital goal for rice breeding. A set of 85 backcross inbred lines derived from the backcross ‘Sasanishiki’ (japonica cv.)/‘Habataki’ (an indica cv. with strong seed storability)//‘Sasanishiki’ was used here to detect quantitative trait locus (QTL) controlling seed storability. Seeds were treated under natural (conventional storage for 48 months) and artificial aging storage conditions (i.e. increased temperature and relative humidity) and germination rate was used to evaluate seed storability. A total of thirteen QTLs for seed storability were identified on chromosomes 1, 2, 3, 4, 5, 7, 11 and 12, respectively. Among them, two QTLs, viz. qSSh-2-1 and qSSh-2-2, were repeatedly detected in both treatment conditions. In contrast, four (qSSh-4, qSSs-5-1, qSSs-5-2 and qSSh-12) and seven QTLs (qSSh-1, qSSh-3-1, qSSh-3-2, qSSh-3-3, qSSh-7-1, qSSh-7-2 and qSSh-11) were detected only once in natural and artificial aging treatments, respectively. The ‘Habataki’-derived alleles were observed to increase seed storability at all the loci except qSSs-5-1 and qSSs-5-2. The existence of QTLs qSSh-1, qSSh-3-1, qSSh-3-2, qSSh-3-3, qSSh-4, qSSh-7-1, qSSh-7-2 and qSSh-11 was confirmed using Habataki chromosome segment substitution lines in a Sasanishiki genetic background. These results provide an opportunity for map-based cloning of major QTLs for seed storability, thereby gaining an understanding of seed storability in rice and possibilities for its improvement.

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Abbreviations

QTL:

Quantitative trait locus

BIL:

Backcross inbred line

CSSL:

Chromosome segment substitution line

qSSh :

Quantitative trait loci of Habataki allele enhancing seed storability

qSSs :

Quantitative trait loci of Sasanishiki allele enhancing seed storability

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Acknowledgments

The research described in this paper was financially supported by a project (No. 2014ZX08001-006) from Ministry of Agriculture of China for transgenic research, Key Laboratory of Biology, Genetics and Breeding of Japonica Rice in Mid-lower Yangtse River, Ministry of Agriculture, P.R.China and Jiangsu Qinglan Project.

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China

    Ngo Thi Hang, Qiuyun Lin, Linglong Liu, Xi Liu, Shijia Liu, Wenyan Wang, Linfang Li, Niqing He, Zhou Liu, Ling Jiang & Jianmin Wan

  2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Jianmin Wan

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  1. Ngo Thi Hang
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  2. Qiuyun Lin
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  3. Linglong Liu
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  4. Xi Liu
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  5. Shijia Liu
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  6. Wenyan Wang
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  7. Linfang Li
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  8. Niqing He
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  9. Zhou Liu
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  10. Ling Jiang
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  11. Jianmin Wan
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Correspondence to Ling Jiang.

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Ngo Thi Hang and Lin Qiuyun have contributed equally to the article.

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Hang, N.T., Lin, Q., Liu, L. et al. Mapping QTLs related to rice seed storability under natural and artificial aging storage conditions. Euphytica 203, 673–681 (2015). https://doi.org/10.1007/s10681-014-1304-0

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  • DOI: https://doi.org/10.1007/s10681-014-1304-0

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