Abstract
The purple-leaf phenotype in Chinese cabbage is due to the accumulation of anthocyanin. To investigate the pattern of inheritance of this trait in Brassica rapa, F1, F2 and backcross (BC) populations were constructed by crossing 09N-742, a pak-choi inbred line that has purple leaves, with a green-leaved Chinese cabbage inbred line, 09-680. Using a segregating F2 population, we identified a single dominant gene, BrPur, for purple leaf, and mapped the gene to a locus on linkage group A03 of B. rapa. Furthermore, sequences from BAC clones and other sources were used to develop molecular marker loci that are tightly linked to BrPur by using a BC1 population of 1,152 individuals. BrPur was assigned to a locus between Indel markers BVRCPI613 and BVRCPI431, which defined a genetic interval of 0.6 cM and a genomic region of 54.87 kb. Sequence analysis of this chromosomal region revealed seven open reading frames. These results provide a foundation for map-based cloning, identification, and functional analysis of the BrPur gene in B. rapa.
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Acknowledgments
This work was supported in part by Grants from the National High Technology Research and Development Program of China (973,863 Program) (2012CB113906, 2012AA100105), Beijing Natural Science Foundation (6132010), the National Science and Technology Foundation (2012BAD50G01), the earmarked fund for Modern Agro-industry Technology Research System (CARS-25-A-11), and the Advanced Program of Science and Technology Activities of Overseas Personnel in Beijing City of 2012.
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Deshuang Zhang and Weihong Wang contributed equally to this work.
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Wang, W., Zhang, D., Yu, S. et al. Mapping the BrPur gene for purple leaf color on linkage group A03 of Brassica rapa . Euphytica 199, 293–302 (2014). https://doi.org/10.1007/s10681-014-1128-y
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DOI: https://doi.org/10.1007/s10681-014-1128-y