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Improved methods for the purification and enrichment of banana streak virus for antibody production and protein detection

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Abstract

The purification of viruses from bananas is a complex and laborious process due to the large quantities of polysaccharides and secondary metabolites in the plant tissue. The banana streak viruses (BSVs) are generally difficult to purify to a level that allows generation of high quality antisera. This study presents an improved method of purification of Banana streak MY virus (BSMYV), which allowed us to generate highly specific chicken and rabbit antisera and to detect the capsid proteins for the first time. Finally, we demonstrate further enrichment of BSMYV by isoelectric focusing.

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Acknowledgments

JNV and ADWG would like to acknowledge the support of the Cooperative Research Centre for National Plant Biosecurity, established and supported under the Australian Government’s Cooperative Research Centres’ Program. The authors acknowledge Ras Azira Ramli for assistance with the optimisation of ELISA assay using JVQ1 antibody.

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Authors and Affiliations

  1. Cooperative Research Centre for Plant Biosecurity, LPO Box 5012, Bruce, 2617, Australia

    Jenny N. Vo & Andrew D. W. Geering

  2. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, GPO Box 267, Brisbane, QLD, 4001, Australia

    Jenny N. Vo & Andrew D. W. Geering

  3. School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, 4001, Australia

    Nur Nabihah Mahfuz

  4. Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA

    Benham E. L. Lockhart

  5. Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, 2109, Australia

    Jenny N. Vo

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  1. Jenny N. Vo
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  2. Nur Nabihah Mahfuz
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  3. Benham E. L. Lockhart
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  4. Andrew D. W. Geering
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Correspondence to Jenny N. Vo.

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Vo, J.N., Mahfuz, N.N., Lockhart, B.E.L. et al. Improved methods for the purification and enrichment of banana streak virus for antibody production and protein detection. Eur J Plant Pathol 143, 619–626 (2015). https://doi.org/10.1007/s10658-015-0708-6

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  • DOI: https://doi.org/10.1007/s10658-015-0708-6

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