Abstract
Mealybug wilt of pineapple (MWP) is a disease complex whose etiology seems to involve the mealybug Dysmicoccus brevipes and pineapple mealybug wilt-associated virus-1, −2 and/or −3. MWP, the reniform and the root-knot nematodes (RN, RKN) are major problems of pineapple crop worldwide. Although these nematodes are often found in MWP-affected plantations, so far, no study has investigated possible interactions among these pests causing symptoms and affecting the development of pineapple plants. Pineapple plantlets free of diseases and pests (Vitória cultivar) were inoculated with i) RN; ii) RKN; iii) viruliferous mealybugs; iv) RN and viruliferous mealybugs; and v) RKN and viruliferous mealybugs. Uninoculated plants served as controls. After inoculation, the plants were transplanted into microplots, where shoot symptoms and growth were assessed at 9 and 16 months after inoculation. RN, RKN and MWP induced distinct shoot symptoms, which appeared much worse in co-inoculated plants, including the collapse and death of nearly half of the experimental plants at 16 months. RKN, RN and MWP, alone or in combination, reduced the fresh weight of the root system up to 88 % and shoot weight up to 68 %. The interaction among these pests was additive. The results of this experiment emphasize the need for further studies conducted in commercial plantations to understand the interactions among RKN, RN and MWP, and their relative importance, since these are often misdiagnosed and consequently poorly managed.
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Abawi, G. S., & Chen, J. (1998). Concomitant Pathogen and Pest Interactions. American Society of Agronomy Crop Science Society of America. New York State Agricultural Experimental Station, Cornell Universiry, Geneva, USA.
Azevedo, P. V., Souza, C. B., Silva, B. B., & Silva, V. P. R. (2007). Water requirements of pineapple crop grown in a tropical environment. Agricultural Water Management, 88, 201–208.
Back, M. A., Haydock, P. P. J., & Jenkinson, P. (2002). Disease complexes involving plant parasitic nematodes and soil-borne pathogens. Plant Pathology, 5, 683–697.
Cavalcante, U. M. T., Warumby, J. F., Bezerra, J. E. F., & Moura, R. M. (1984). Nematóides associados aos abacaxizeiros no Estado de Pernambuco. Nematologia Brasileira, 8, 39–45.
Costa, D. C., & Matos, A. P. (2000). Nematoses. Embrapa: Mandioca e Fruticultura Tropical, Cruz das Almas – Ba, Circular 2333.
Costa, D. C., Sanches, N. F., & Santos, J. M. (1998). Levantamento de fitonematóides associados ao abacaxizeiro. Revista Brasileira de Fruticultura, 20, 392–396.
Esbenshade, P. R., & Triantaphyllou, A. C. (1985). Use of enzyme phenotypes for identification of Meloidogyne species. Journal of Nematology, 17, 6–20.
Ferreira, T. F., Souza, R. M., Idalino, W. S. S., Ferreira, K. D. S. & Brioso, P. S. T. (2014). Interaction of Pratylenchus brachyurus and Helicotylenchus sp. with mealybug wilt of pineapple in microplots. Nematropica, in press.
Fundação Arthur Bernardes (2007). SAEG – Sistema para Análise Estatísticas. Versão 9.1. Viçosa, Universidade Federal de Viçosa.
Gambley, C. F., Steele, V., Geering, A. D. W., & Thomas, J. E. (2008). The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease. Australasian Plant Pathology, 37, 95–105.
Hernandez-Rodriguez, L., Ramos-Gonzalez, P. L., Garcia-Garcia, G., Zamora, V., Peralta-Martin, A. M., Peña, I., Perez, J. M., & Ferriol, X. (2014). Geographic distribution of mealybug wilt disease of pineapple and genetic diversity of viruses infecting pineapple in Cuba. Crop Protection, 65, 43–50.
IBGE (2010). Instituto Brasileiro de Geografia e Estatística. SIDRA - Banco de Dados Agregados. Produção Agrícola Municipal. http://www.sidra.ibge.gov.br/. Accessed 18 April de abril 2012.
Jenkins, W. R. (1964). A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Disease Report, 48, 692.
Lacerda, J. T., Carvalho, R. A., & Oliveira, E. F. (2009). Cochonilha Dysmicoccus brevipes: a praga cosmopolita da abacaxicultura. Tecnologia e Ciências Agropecuárias, 3, 15–21.
Manso, E. C., Tenente, R. C. V., Ferraz, L. C. B., Oliveira, R. S. O., & Mesquita, R. (1994). Catálogo de Nematoides Fitoparasitos Encontrados associados a Diferentes Tipos de Plantas no Brasil. Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia. EMBRAPA-SPI, 1, 354–355.
Peron, F. N. (2011). Diagnóstico molecular e imunoenzimático de pineapple mealybug wilt-associated vírus (PMWaV) em diferentes genótipos de abacaxizeiro. Master dissertation, Universidade Federal do Espírito Santo, Brazil, 82 pp.
Ramos, M. J. M. R., Monnerat, P. H., Carvalho, A. J. C., Pinto, L. G. R., & Silva, J. A. (2009). Sintomas visuais de deficiência de macronutrientes e de boro em abacaxizeiro ‘Imperial’. Revista Brasileira de Fruticultura, 31, 252–256.
Reinhardt, D. H., & Souza, J. S. (2000). Pineapple industry and research in Brazil. Acta Horticulturae, 529, 57–71.
Rohrbach, K. G., & Apto, W. J. (1986). Nematodes and disease problems of pineapple. Plant Disease Report, 70, 81–87.
Sanches, N. F. (2005). Manejo integrado da cochonilha do abacaxi. Embrapa: Mandioca e Fruticultura Tropical, Cruz das Almas.
Santos, K. C., Andrade, E. C., Souza, F. V. D. & Carvalho, H. L. (2011). Incidência e prevalência do pineapple mealybug wilt associated vírus em germoplasma de abacaxi. Tropical Plant Pathology (supplement), 36, 782. Proceedings of the XLIV Congresso Brasileiro de Fitopatologia, Bento Gonçalves (RS), Brazil.
Sether, D. M., & Hu, J. S. (2002). Yield impact and spread of pineapple mealybug wilt associated virus-2 and mealybug wilt of pineapple in Hawaii. Plant Disease Report, 86, 867–874.
Sether, D. M., Melzer, M. J., Busto, J., Zee, F., & Hu, J. S. (2005). Diversity and mealybug transmissibility of ampeloviruses in pineapple. Plant Disease Report, 89, 450–456.
Sether, D. M., Borth, W. B., Melzer, M. J., & Hu, J. (2010). Spatial and temporal incidences of pineapple mealybug wilt-associated viruses in pineapple planting blocks. Plant Disease, 94, 196–200.
Siddiqi, M. R. (2000). Tylenchida parasites of plants and insects (2nd ed.). Wallingford: CABI Publishing.
Sipes, B. S., & Schmitt, D. P. (1994). Evaluation of pineapple, Ananas comosus, for host-plant resistance and tolerance to Rotylenchulus reniformis and Meloidogyne javanica. Nematropica, 24, 113–121.
Sipes, B. S., Sether, D. M., & Hu, J. S. (2002). Interactions between Rotylenchus reniformis and Pineapple mealybug wilt associated virus-1 in pineapple. Plant Disease Report, 86, 933–938.
Sipes, B. S., Caswell-Chen, E. P., Sarah, J. L., & Apt, W. J. (2005). Nematode parasites of pineapple. In M. Luc, R. A. Sikora, & J. Bridge (Eds.), Plant parasitic nematodes in subtropical and tropical agriculture (pp. 709–731). Wallingford: CABI Publishing.
Stirling, G. R., & Kopittke, R. (2000). Sampling procedures and damage threshold for root-knot nematode (Meloidogyne javanica) on pineapple. Australian Journal of Experimental Agriculture, 40, 1003–1010.
Veloso, C. A. C., Oeiras, A. H. L., Carvalho, E. J. M., & Souza, F. R. S. (2001). Resposta do abacaxizeiro à adição de nitrogênio, potássio e calcário em latossolo amarelo do Nordeste Paraense. Revista Brasileira Fruticultura, 23, 396–402.
Zem, A. C., & Reinhardt, D. H. R. C. (1978). Nematoides associados à cultura do abacaxi no Estado da Bahia. Sociedade Brasileira de Nematologia, 3, 17–20.
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We thank Prof. Paulo Sérgio T. Brioso (UFRuRJ, Brazil) for conducting the RT-PCR test for PMWaV identification.
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Ferreira, T.d.F., Souza, R.M., Ferreira, K.D.d.S. et al. Interaction of Rotylenchulus reniformis and Meloidogyne javanica with mealybug wilt of pineapple, in microplots. Eur J Plant Pathol 141, 761–768 (2015). https://doi.org/10.1007/s10658-014-0576-5
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DOI: https://doi.org/10.1007/s10658-014-0576-5