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Characterization and mapping of a spotted-leaf genotype, spl Y181 that confers blast susceptibility in rice

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Abstract

A spotted-leaf mutant Y181, produced by 60Coγ-radiation treatment of Zixuan1, was identified in rice. The result of TEM demonstrated the number of starch grains decreased significantly, the distribution and volumes of osmiophilic granules and lipid balls were larger in the leaf cells around the lesion mimics, the chloroplasts in mesophyll cells disappeared and just leaving a few rupture thylakoid membranes in the cytoplasm and some transmutative starch granules in Y181. Compared with the wild type, the content of chlorophyll was decreased significantly, while the H2O2 content, and SOD, POD and CAT activity were obviously increased in mutant. DAB staining and H2O2 treatment further revealed oxidative burst in spotted leaves. Expression of lesion-related gene showed RuBisCo-L, RuBisCo-S, PDI and TPX was down-regulated, while PBZ1 was up-regulated in the leaf cell at the lesion mimics. Disease reaction to Magnaporthe grisea indicated that the resistance of Y181 was reduced. Genetic analysis showed that the mutant was controlled by a recessive nuclear gene, which was delimited to a 390 kb region between CH12-936 and RM7195.

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Acknowledgments

This study was supported by a transgenosis research with grant No. 2008 ZX08001-002 and 2011 ZX08001-002 from the Ministry of Agriculture, China. This work was also supported by the center of instrumental analysis in Zhejiang University, we are grateful to Ms. Y. Xu for her generous help.

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Authors and Affiliations

  1. College of life science, Jinggangshan University, Ji’an, 343009, China

    Huimin Sun

  2. College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, China

    Huimin Sun & Xiaohua Pan

  3. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China

    Jiejing Mao, Chunjiao Zhang, Chenchen Zhao & Gang Pan

  4. Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, China

    Bo Lan

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  1. Huimin Sun
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  2. Jiejing Mao
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Correspondence to Gang Pan or Xiaohua Pan.

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Sun, H., Mao, J., Lan, B. et al. Characterization and mapping of a spotted-leaf genotype, spl Y181 that confers blast susceptibility in rice. Eur J Plant Pathol 140, 407–417 (2014). https://doi.org/10.1007/s10658-014-0476-8

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  • DOI: https://doi.org/10.1007/s10658-014-0476-8

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