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Honeybee tracking with microchips: a new methodology to measure the effects of pesticides

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Abstract

Losses of foraging bees are sometimes attributed to altered flight pattern between a meliferous plant treated with an insecticide and the hive. Only a limited number of studies has investigated the impact of pesticides on homing flight due to the difficulty of measuring the flight time between the food source and the hive. Monitoring the flights of the foraging bees needs their individual identification. The number of bees monitored simultaneously and the time span during which observations can be made limit most of the monitoring techniques. However, techniques of automatic tracking and identification of individuals have the potential to revolutionize the study of the ecotoxicological effects of xenobiotics on the bee behaviors. Radio Frequency Identification (RFID) offer numerous advantages such as an unlimited number of codes, a large number of simultaneous recording, and a quick reading, especially through materials (e.g., wood). The aim of this study was to show how the RFID device can be used to study the effects of pesticides on both the behavioral traits and the lifespan of bees. In this context, we have developed a method under tunnel to automatically record the displacements of foragers individualized with RFID tags and to detect the alteration of the flight pattern between an artificial feeder and the hive. Fipronil was selected as test substance due to the lack of information on the effects of this insecticide on the foraging behavior of free-flying bees. We showed that oral treatment of 0.3 ng of fipronil per bee (LD50/20) reduced the number of foraging trips. The strengths of our approach were briefly discussed.

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Acknowledgments

This work was supported by grants from the European Community program (1221/97) for French beekeeping coordinated by French Ministry of Agriculture.

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Authors and Affiliations

  1. ACTA, UMT PrADE, UMR 406, Site Agroparc, 84914, Avignon Cedex 9, France

    Axel Decourtye

  2. ACTA, Institut Claude Bourgelat, 1 Avenue C. Bourgelat, 69280, Marcy l’Etoile, France

    Camille Bagnis & Julie Fourrier

  3. CTIS, 3 Chemin de la gravière, 69140, Rillieux La Pape, France

    James Devillers

  4. INRA, Unité expérimentale d’entomologie Le Magneraud, 17700, Surgères, France

    Pierrick Aupinel

  5. ACTA, UMR 1248 AGIR, B.P. 52627, 31326, Castanet Tolosan Cedex, France

    François Brun

  6. CNRS, Centre de Recherches sur la Cognition Animale, Université Paul Sabatier, 118 Route de Narbonne, 31062, Toulouse Cedex, France

    Monique Gauthier

Authors
  1. Axel Decourtye
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  2. James Devillers
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  3. Pierrick Aupinel
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  4. François Brun
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  5. Camille Bagnis
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  6. Julie Fourrier
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  7. Monique Gauthier
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Correspondence to Axel Decourtye.

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Decourtye, A., Devillers, J., Aupinel, P. et al. Honeybee tracking with microchips: a new methodology to measure the effects of pesticides. Ecotoxicology 20, 429–437 (2011). https://doi.org/10.1007/s10646-011-0594-4

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